Regulation of activity and transcript levels of NR in rice (Oryza sativa): Roles of protein kinase and G-proteins

Ali, Ahmad; Sivakami, S.; Raghuram, Nandula

doi:10.1016/j.plantsci.2006.10.003

Cited by 22 publications

(17 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…and cytochrome P450 s. These are significant in the light of our earlier findings on the upregulation of nitrate reductase gene expression by cholera toxin in maize (Raghuram et al 1999 ) and rice (Ali et al 2007 ) and its down-regulation in the GPA1 mutant (unpublished data). In addition, our finding on the regulation of LBD29 suggests a possible role of GPA1 in lateral root development.…”

Section: Nitrate Responsesupporting

confidence: 61%

“…The α subunit of plant heterotrimeric G-proteins has been implicated in various roles such as hypocotyl elongation, hook angle, rosette diameter, leaf shape (Ullah et al 2001 ), ABA inhibition of stomatal opening (Wang et al 2001 ), positive regulation of stomatal density (Zhang et al 2008 ), pollen tube development (Wu et al 2007 ), auxin mediated cell division, lateral root proliferation (Ullah et al 2003 ), plant height (Fujisawa et al 1999 ;Ueguchi-Tanaka et al 2000 ), grain size (Oki et al 2005 ), sphingolipid signaling (Coursol et al 2003 ), resistance to pathogens (Komatsu et al 2004 ;Suharsono 2002 ), sugar perception (Huang et al 2006 ), light regulation of nitrate reductase gene expression (Ali et al 2007 ;Raghuram et al 1999 ) and blue light-induced production of phenylalanine (Warpeha et al 2006(Warpeha et al , 2007. The other two G-protein subunits, β and γ, have also been implicated in several responses like cell division (Ullah et al 2001 ), organ shape and size Lease et al 2001 ;Li et al 2012 ), gibberellin ( GA) biosynthesis (Chen et al 2004 ;Ullah et al 2003 ), control of sugar, GA gibberellin or brassinosteroid mediated inhibition of germination (Chen et al 2004 ), control of guard cell ion channels and response to ABA (Chakravorty et al 2011 ), resistance to necrotrophic pathogens, jasmonate signaling (Ishikawa 2009 ;Llorente et al 2005 ;Trusov et al 2006 ) and oxidative stress responses (Joo et al 2005 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana

et al. 2015

Self Cite

View full text Add to dashboard Cite

Heterotrimeric G-proteins are implicated in several plant processes, but the mechanisms of signal-response coupling and the roles of G-protein coupled receptors in general and GCR1 in particular, remain poorly understood. We isolated a knock-out mutant of the Arabidopsis G-protein α subunit (gpa1-5) and analysed its transcriptome to understand the genomewide role of GPA1 and compared it with that of our similar analysis of a GCR1 mutant (Chakraborty et al. 2015, PLoS ONE 10(2):e0117819). We found 394 GPA1-regulated genes spanning 79 biological processes, including biotic and abiotic stresses, development, flavonoid biosynthesis, transcription factors, transporters and nitrate/phosphate responses. Many of them are either unknown or unclaimed explicitly in other published gpa1 mutant transcriptome analyses. A comparison of all known GPA1-regulated genes (including the above 394) with 350 GCR1-regulated genes revealed 114 common genes. This can be best explained by GCR1-GPA1 coupling, or by convergence of their independent signaling pathways. Though the common genes in our GPA1 and GCR1 mutant datasets constitute only 26% of the GPA1-regulated and 30% of the GCR1-responsive genes, they belong to nearly half of all the processes affected in both the mutants. Thus, GCR1 and GPA1 regulate not only some common genes, but also different genes belonging to the same processes to achieve similar outcomes. Overall, we validate some known and report many hitherto unknown roles of GPA1 in plants, including agronomically important ones such as biotic stress and nutrient response, and also provide compelling genetic evidence to revisit the role of GCR1 in G-protein signalling.

show abstract

Section: Nitrate Responsesupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reduction of nitrate to ammonium is catalyzed by nitrate reductase. The activity and transcript level of nitrate reductase in rice plant was suggested to be influenced by G-protein and protein kinase (Ali et al 2007). The average of nitrate reductase activity in black rice plants treated with paclobutrazol and blue light is presented in Figure 8.…”

Section: Activity Of Nitrate Reductasementioning

confidence: 99%

EFFECTS OF BLUE LIGHT AND PACLOBUTRAZOL ON SEED GERMINATION, VEGETATIVE GROWTH AND YIELD OF BLACK RICE (Oryza sativa L. 'Cempo Ireng')

Dewi

Agustina²,

Nurmalika³

2017

BIOTROPIA

View full text Add to dashboard Cite

Black rice (Oryza sativa L. "Cempo Ireng") is one of local rice varieties in Sleman Regency, Yogyakarta. The black color is caused by high anthocyanin content which is important source of antioxidant. The cultivation of black rice is still limited due to its tall phenotype, long vegetative stage and low productivity compared to white rice. Paclobutrazol is a growth retardant causing dwarfing in several crop plants and reducing lodging. Blue light can improve plant quality. This research was aimed to evaluate the effect of blue light and paclobutrazol on seed germination, vegetative growth and yield of black rice. The results showed that the average of seed germination as well as the activity of α-amylase of seeds subjected to blue light were lower compared to those subjected to sunlight; however, paclobutrazol concentrations did not affect seed germination percentage. The height of rice plants treated with paclobutrazol decreased in accordance with the increase of paclobutrazol concentration. Chlorophyll content and tiller numbers increased by paclobutrazol treatment of 12.5 ppm. Nitrate reductase activity was higher in rice seedlings subjected to blue light compared to those subjected to sunlight. Iron (Fe) content of rice plants treated with 25 or 50 ppm paclobutrazol increased compared to control. It was concluded that paclobutrazol application of 12.5 ppm already reduced plant height. The higher concentration of paclobutrazol applied the greater reduction on plant height was observed. Blue light treatment applied during black rice seed germination slightly reduced germination percentage and α-amylase activity in the germinated seeds. However, blue light treatment combined with paclobutrazol application during black rice seed germination increased chlorophyll content, tiller numbers and Fe content in black rice grain.

show abstract

“…Studies some years ago had shown that the protein phosphatase inhibitors okadaic acid and microcystin prevented greening in etiolated maize, and expression of several photosynthesis-related genes were inhibited by okadaic acid in rice [23,24]. In leaves of spinach, maize and rice, okadaic acid and microcystin were clearly shown to influence NR mRNA and NR protein levels [25][26][27]. In maize, okadaic acid completely inhibited the lightinduced increase in NR mRNA level [28].…”

Section: Introductionmentioning

confidence: 99%

Signalling cascades integrating light-enhanced nitrate metabolism

Lillo

2008

Biochemical Journal

167

128

View full text Add to dashboard Cite

In higher plants, light is crucial for regulation of nitrate uptake, translocation and assimilation into organic compounds. Part of this metabolism is tightly coupled to photosynthesis because the enzymes involved, nitrite reductase and glutamate synthase, are localized to the chloroplasts and receive reducing power from photosynthetic electron transport. However, important enzymes in nitrate acquisition and reduction are localized to cellular compartments other than chloroplasts and are also up-regulated by light, i.e. transporters in cell and organellar membranes and nitrate reductase in the cytosol. This review describes the different light-dependent signalling cascades regulating nitrate metabolism at the transcriptional as well as post-transcriptional level, and how reactions in different compartments of the cell are co-ordinated. Essential players in this network are phytochrome and HY5 (long hypocotyls 5)/HYH (HY5 homologue)-dependent signalling pathways, the energy-related AMPK (AMP-activated protein kinase) protein kinase homologue SNRK1 (sucrose non-fermenting kinase 1-related kinase), chloroplastic thioredoxins and the prokaryotically originated PII protein. A complex light-dependent network of regulation emerges, which appears to be necessary for optimal nitrogen assimilation and for avoiding the accumulation of toxic intermediates and side products, such as nitrite and reactive oxygen compounds.

show abstract

Regulation of activity and transcript levels of NR in rice (Oryza sativa): Roles of protein kinase and G-proteins

Cited by 22 publications

References 15 publications

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana

EFFECTS OF BLUE LIGHT AND PACLOBUTRAZOL ON SEED GERMINATION, VEGETATIVE GROWTH AND YIELD OF BLACK RICE (Oryza sativa L. 'Cempo Ireng')

Signalling cascades integrating light-enhanced nitrate metabolism

Contact Info

Product

Resources

About