Regulation of active Na+-K+ transport in skeletal muscle

Clausen, Torben

doi:10.1152/physrev.1986.66.3.542

Cited by 301 publications

(187 citation statements)

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“…In addition, ADR resulted in an increase of the intracellular K § activity (aKi) as measured in rat soleus muscle. These results confirm earlier measurements of cellular Na + content, serum K + concentrations, and ofNa + and K + fluxes in rat soleus muscle (Dockry et al 1966;Clausen andFlatman 1977, 1980;Pfliegler et al 1983). Furthermore, the magnitude of the average decreases of aNai (1.73 mM in rat soleus and 2.3 mM in human intercostal muscle) very closely resembled catecholamine-induced decreases of aNal as measured with Na +-sensitive micro-electrodes in different heart tissues as dog heart Purkinje and ventricular trabecular muscle fibers (Wasserstrom et al 1982), canine Purkinje fibers (Lee and Vasalle 1983) and isolated myocytes of the rabbit (D6silets and Baumgarten 1986).…”

Section: Discussionsupporting

confidence: 90%

“…The close relationship between the time courses of the observed Na + and K + activity and membrane potential changes favours a stimulation of the electrogenic Na+/K + pump as the ionic mechanism for the effects of ADR as proposed for the majority of previous investigations (Clausen 1986). A shift of the K + equilibrium potential towards a more negative value also contributes to the membrane hyperpolarization during ADR (Clausen and Flatman 1977).…”

Section: Discussionsupporting

confidence: 64%

“…Catecholamines have a facilitating action on the active Na +/ K § transport in striated muscle (Clausen 1986). This stimulating effect is relevant for the K § homeostasis in skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

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Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

Ballanyi

Grafe

1988

Pflugers Arch.

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Abstract. To study the stimulating effect of adrenaline (ADR) on active Na+/K + transport we used double-barrelled ion-sensitive micro-electrodes to measure the activities of extracellular K + (aKe) and intracellular Na + (aNai) in isolated preparations of rat soleus muscle, normal human intercostal muscle and one case of hyperkalemic periodic paralysis (h.p.p.). In these preparations, bath-application of ADR (10 .6 M) resulted in a membrane hyperpolarization and transient decreases aKe and aNal which could be blocked by ouabain (3 x 10 .4 M). In the h.p.p, muslce a continuous rise of aNal induced by elevation of aKe to 5.2 mM could be stopped by ADR. In addition, the intracellular K § activity (aKi), the free intracellular Ca 2 + concentration (pCai) and intracellular pH (pHi) were monitored in rat soleus muscle. During ADR aKi increased, pHi remained constant and intracellular Ca z § apparently decreased. In conclusion, our data show that ADR primarily stimulates the Na+/K § pump in mammalian skeletal muscle. This stimulating action is not impaired in the h.p.p, muscle.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 64%

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Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

Ballanyi

Grafe

1988

Pflugers Arch.

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“…Among the best-documented responses in skeletal muscle to insulin are the activation of glucose transport and the Na,KATPase [1][2][3][4]. Skeletal muscle expresses both the GLUT1 and GLUT4 glucose transporters but it is the mobilization of GLUT4, from an intracellular store to the plasma membrane (PM), that explains the observed increase in glucose transport in response to insulin binding [5][6][7][8].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Sedimentation and immunological analyses of GLUT4 and α2‐Na,K‐ATPase subunit‐containing vesicles from rat skeletal muscle: evidence for segregation

Aledo

Hundal

1995

FEBS Letters

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In skeletal muscle insulin induces the translocation of both the GLUT4 glucose transporter and the 62 subunit of the Na,K-ATPase from an intraceHnlar membrane (154) compartment to the plasma membrane (PM). Fractionation studies of rat skeletal muscle using a discontinuous sucrose gradient have indicated that the insulin-induced loss of both proteins occurs from a fraction containing intracellular membranes (IM) of common density. This raises the possibility that both proteins may be colocalized in a single intracellular compartment or are present in separate membrane vesicles that are of similar buoyant density. In this study we report that membrane vesicles from the insulinresponsive IM fraction can in fact be separated on the basis of differences in their sedimentation velocities; immunoblot analyses of fractions collected from a sucrose velocity gradient revealed the presence of two separate peaks for GLUT4 and the a2 subunit of the Na,K-ATPase. One of these peaks representing a fast sedimenting population of vesicles (with a sedimentation coefficient of 2697 + 57 S) reacted against antibodies to the 62 subunit of the Na,K-ATPase, whereas, the second peak contained a population of much slower sedimenting vesicles (with a sedimentation coefficient of 209 -+ 4 S) were practically devoid of the a2-subunit. By contrast, the slow sedimenting vesicles were enriched by ~32-fold in GLUT4 relative to the starting IM fraction when the fractional protein content was taken into account. Immunoprecipitation of GLUT4-containing vesicles from the insulin-sensitive IM fraction revealed that no immunoreactivity towards either the a2 or the ~1 subunits of the Na,K-ATPase could be observed, signifying that the insulin-responsive subunits of the Na,K-ATPase and GLUT4 were present in different membrane vesicles and that it was unlikely, therefore, that the insulin-induced redistribution of these proteins to the PM occurs from a common intracellular pool.

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“…The uptake of glucose decreases and glycolysis increases rapidly (7). These morphologic and metabolic changes may relate to the water metabolism in the skeletal muscle, in which functional disability of sodium/potassium adenosine triphosphatase (Na/K-ATPase, also known as the sodium-potassium pump) (8), muscle cell membrane permeability change (9), loss of neurotrophic factor (10), increase of blood volume induced by interruption of sympathetic vasoconstriction (11), and disuse atrophy induced by immobilization all contribute to the increase of extracellular water volume.…”

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confidence: 99%

Line scan diffusion spectrum of the denervated rat skeletal muscle

Yamabe

Nakamura

Oshio

et al. 2007

Magnetic Resonance Imaging

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Purpose: To detect the earlier changes of the skeletal muscle of rats after peripheral nerve injury by measuring the apparent diffusion coefficient (ADC) on diffusion MR spectroscopy. Materials and Methods:The posterior tibial nerve was transected in six rats (nerve transection group) and was only dissected in six rats (control group). At one, three, five, seven, 14, and 28 days after the surgery, both the T2 value and the ADC of gastrocnemius muscle were measured using a line-scan diffusion spectrum on a 1.5T clinical MR imager on both groups. Results:In the nerve transection group, the T2 ratio compared to the contralateral side increased gradually over four weeks after the transection, while the ADC ratio increased right after the surgery and began to decrease at five days. Four weeks after the transection, the ADC ratio returned to normal while the T2 ratio stayed at a high value. The control group indicated an almost constant T2 and ADC ratio during the experimental periods. Conclusion:The ADC of the skeletal muscle increased quickly after the transection of the dominant peripheral nerve and was detectable one day after the surgery. Diffusion MRI can be a useful tool for early detection of peripheral nerve injury instead of T2-weighted MRI or electromyography (EMG).

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Regulation of active Na+-K+ transport in skeletal muscle

Cited by 301 publications

References 0 publications

Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

Sedimentation and immunological analyses of GLUT4 and α2‐Na,K‐ATPase subunit‐containing vesicles from rat skeletal muscle: evidence for segregation

Line scan diffusion spectrum of the denervated rat skeletal muscle

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