2004
DOI: 10.1016/j.femsle.2004.09.033
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Regulation of acetate and acetyl-CoA converting enzymes during growth on acetate and/or glucose in the halophilic archaeonHaloarcula marismortui

Abstract: Haloarcula marismortui formed acetate during aerobic growth on glucose and utilized acetate as growth substrate. On glucose/acetate mixtures diauxic growth was observed with glucose as the preferred substrate. Regulation of enzyme activities, related to glucose and acetate metabolism was analyzed. It was found that both glucose dehydrogenase (GDH) and ADP-forming acetyl-CoA synthetase (ACD) were upregulated during periods of glucose consumption and acetate formation, whereas both AMP-forming acetyl-CoA synthet… Show more

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Cited by 27 publications
(14 citation statements)
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References 25 publications
(37 reference statements)
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“…Both genes, encoding isocitrate lyase (SSO1333) and malate synthase (SSO1334), are consecutively colocalized in the genome but are separated by Ͼ450 bp and each exhibits its own promotor region including an upstream TATA box not forming an operon (291). The presence of the entire glyoxylate shunt in glucose-grown cells might be due to the reutilization of acetate excreted during exponential growth on glucose in the stationary phase, as also described for haloarchaea (357)(358)(359).…”
Section: Conversion Of Glycolaldehyde To Malatementioning
confidence: 80%
“…Both genes, encoding isocitrate lyase (SSO1333) and malate synthase (SSO1334), are consecutively colocalized in the genome but are separated by Ͼ450 bp and each exhibits its own promotor region including an upstream TATA box not forming an operon (291). The presence of the entire glyoxylate shunt in glucose-grown cells might be due to the reutilization of acetate excreted during exponential growth on glucose in the stationary phase, as also described for haloarchaea (357)(358)(359).…”
Section: Conversion Of Glycolaldehyde To Malatementioning
confidence: 80%
“…The ecSCS has a high specificity for succinylCoA (22) but converts small aliphatic CoA esters (22) like ckcACD1 and other ACDs (2, 3, 5-7, 20, 21, 24, 37-39) as well. Interestingly, members of the ACD family are also able to transform aromatic CoA thioesters (3,24,39), although catalytic activity toward succinyl-CoA has only been described a few times (3,25,40). The high specificity of SCS enzymes for succinyl-CoA does make a lot of sense because this enzyme family is a central and, as such, an optimized element within the TCA cycle.…”
Section: Discussionmentioning
confidence: 99%
“…Brasen and Schonheit Schonheit 2001, Brasen andSchonheit 2004) During growth on glucose, these halophiles excreted acetate into the media and exhibited ADP-forming acetyl-CoA synthetase activity (ADP-ACS; acetyl-CoA + ADP + P i ↔ acetate + ATP + CoA) but not ACS activity. Upon entry into stationary phase, ACS activity was induced and the excreted acetate was consumed.…”
Section: Discussionmentioning
confidence: 99%