Regulation of 1D-myo-Inositol-3-Phosphate Synthase in Yeast

Nunez, Lilia R.; Henry, Susan A.

doi:10.1007/0-387-27600-9_6

Cited by 7 publications

(3 citation statements)

References 134 publications

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“…Here, we found that the transcription factors FHY3 and FAR1 positively regulate lightinduced expression of MIPS1 through FBS cis-elements in Arabidopsis (Figure 6A), which is completely distinct from the transcriptional regulation of MIPS in other model systems. In yeast, two basic helix-loop-helix (bHLH) transcription factors, Ino2p and Ino4p, bind as a heterodimer to two cis-regulatory (UAS INO ) elements in the INO1 promoter and activate the transcription of INO1 in the absence of inositol in the medium (Loewy and Henry, 1984;Ambroziak and Henry, 1994;Nunez and Henry, 2006;Chen et al, 2007). In response to exogenous inositol, the transcriptional repressor Opi1 rapidly translocates into the nucleus where it inhibits the transcriptional activator complex Ino2p-Ino4p and represses INO1 transcription (Loewen et al, 2004(Loewen et al, , 2012.…”

Section: Transcriptional Regulation Of Mips1 In Arabidopsismentioning

confidence: 99%

Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo -Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1

Tian

Lin

et al. 2016

Molecular Plant

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myo-Inositol-1-phosphate synthase (MIPS) catalyzes the limiting step of inositol biosynthesis and has crucial roles in plant growth and development. In response to stress, the transcription of MIPS1 is induced and the biosynthesis of inositol or inositol derivatives is promoted by unknown mechanisms. Here, we found that the light signaling protein FAR-RED ELONGATED HYPOCOTYL3 (FHY3) and its homolog FAR-RED IMPAIRED RESPONSE1 (FAR1) regulate light-induced inositol biosynthesis and oxidative stress responses by activating the transcription of MIPS1. Disruption of FHY3 and FAR1 caused light-induced cell death after dark-light transition, precocious leaf senescence, and increased sensitivity to oxidative stress. Reduction of salicylic acid (SA) accumulation by overexpression of SALICYLIC ACID 3-HYDROXYLASE largely suppressed the cell death phenotype of fhy3 far1 mutant plants, suggesting that FHY3- and FAR1-mediated cell death is dependent on SA. Furthermore, comparative analysis of chromatin immunoprecipitation sequencing and microarray results revealed that FHY3 and FAR1 directly target both MIPS1 and MIPS2. The fhy3 far1 mutant plants showed severely decreased MIPS1/2 transcript levels and reduced inositol levels. Conversely, constitutive expression of MIPS1 partially rescued the inositol contents, caused reduced transcript levels of SA-biosynthesis genes, and prevented oxidative stress in fhy3 far1. Taken together, our results indicate that the light signaling proteins FHY3 and FAR1 directly bind the promoter of MIPS1 to activate its expression and thereby promote inositol biosynthesis to prevent light-induced oxidative stress and SA-dependent cell death.

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Section: Transcriptional Regulation Of Mips1 In Arabidopsismentioning

confidence: 99%

Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo -Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1

Tian

Lin

et al. 2016

Molecular Plant

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“…To date, most studies have dealt with inositol depletion and its effects in the context of the phosphoinositide cycle. However, inositol is a key component of important intracellular signalling molecules that affect phospholipid synthesis, the UPR (unfolded protein response) and protein secretion [17,[31][32][33]. Indeed, Jesch et al [34] reported altered expression of more than 712 genes in response to inositol.…”

Section: Consequences Of Inositol Depletionmentioning

confidence: 95%

Cellular consequences of inositol depletion

Deranieh

Greenberg

2009

Biochemical Society Transactions

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The inositol-depletion hypothesis was suggested to explain the therapeutic mechanism of mood-stabilizing drugs. Focus was previously on the phosphatidylinositol signalling pathway and on the regulatory roles of Ins(3,4,5)P(3) and DAG (diacylglycerol). Recent findings indicate that inositol and inositol-containing molecules, including phosphoinositides and inositol phosphates, have signalling and regulatory roles in many cellular processes. This suggests that depleting inositol may lead to perturbation of a wide range of cellular functions, at least some of which may be associated with bipolar disorder.

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“…Surprisingly, regulation of inositol synthesis in mammalian cells has not been well studied. In contrast, inositol synthesis has been well characterized in the yeast Saccharomyces cerevisiae [55][56][57].…”

Section: • • Vpa and Lithium Inhibit Inositol Synthesismentioning

confidence: 99%

Inositol depletion, GSK3 inhibition and bipolar disorder

Greenberg

2016

Future Neurol.

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Valproic acid and lithium are widely used to treat bipolar disorder, a severe illness characterized by cycles of mania and depression. However, their efficacy is limited, and treatment is often accompanied by serious side effects. The therapeutic mechanisms of these drugs are not understood, hampering the development of more effective treatments. Among the plethora of biochemical effects of the drugs, those that are common to both may be more related to therapeutic efficacy. Two common outcomes include inositol depletion and GSK3 inhibition, which have been proposed to explain the efficacy of both valproic acid and lithium. Here, we discuss the inositol depletion and GSK3 inhibition hypotheses, and introduce a unified model suggesting that inositol depletion and GSK3 inhibition are inter-related.

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Regulation of 1D-myo-Inositol-3-Phosphate Synthase in Yeast

Cited by 7 publications

References 134 publications

Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo -Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1

Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo -Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1

Cellular consequences of inositol depletion

Inositol depletion, GSK3 inhibition and bipolar disorder

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