Regulation by Interferon <i>γ</i> of Function in the Acute Monocytic Leukemia Cell Line, THP-1

Gaffney, Edwin V.; Lingenfelter, Susan E.; Koch, G; Lisi, Paolo; Chu, Cheng wei; Tsai, Shiow-Chuan

doi:10.1002/jlb.43.3.248

“…With IFN-␥ stimulation, the human monocytic cell line THP-1 expresses a number of genes associated with the antigen-presenting function of macrophages, including major histocompatibility complex class II (MHC-II), CD74 (MHC-II-associated invariant chain), and interleukin-1␤ (7,26; our unpublished observation). To identify genes involved in the function of macrophages, we had previously carried out PCR-based cDNA subtraction and subsequent differential display on mRNA obtained from IFN-␥-treated and untreated THP-1 cells.…”

mentioning

confidence: 86%

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Senju

¹

,

Iyama²,

Kaneko

³

et al. 2000

Molecular and Cellular Biology

View full text Add to dashboard Cite

We previously identified a gene encoding a putative GTPase, GTPBP1, which is structurally related to elongation factor 1␣, a key component of protein biosynthesis machinery. The primary structure of GTPBP1 is highly conserved between human and mouse (97% identical at the amino acid level). Expression of this gene is enhanced by gamma interferon in a monocytic cell line, THP-1. Although counterparts of this molecule in Caenorhabditis elegans and Ascaris suum have also been identified, the function of this molecule remains to be clarified. In the present study, our immunohistochemical analyses on mouse tissues revealed that GTPBP1 is expressed in some neurons and smooth muscle cells of various organs as well as macrophages. Immunofluorescence analyses revealed that GTPBP1 is localized exclusively in cytoplasm and shows a diffuse granular network forming a gradient from the nucleus to the periphery of the cells in smooth muscle cell lines and macrophages. To investigate the physiological role of GTPBP1, we used targeted gene disruption in embryonic stem cells to generate GTPBP1-deficient mice. The mutant mice were born at the expected Mendelian frequency, developed normally, and were fertile. No manifest anatomical or behavioral abnormality was observed in the mutant mice. Functions of macrophages, including chemotaxis, phagocytosis, and nitric oxide production, in mutant mice were equivalent to those seen in wild-type mice. No significant difference was observed in the immune response to protein antigen between mutant mice and wild-type mice, suggesting normal function of antigen-presenting cells of the mutant mice. The absence of an eminent phenotype in GTPBP1-deficient mice may be due to functional compensation by GTPBP2, a molecule we recently identified which is similar to GTPBP1 in structure and tissue distribution.

show abstract

“…Previous studies have shown that several cytokines and/ or hormones may induce differentiation of the THP-1 cell line along the monocyte/macrophage pathway. The triggering stimuli include interferon-gamma (IFN-g) [Gaffney et al, 1988], 1a,25 dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) [Vey et al, 1992], as well as pharmacological agents such as retinoic acid and 12-O-tetradecanoylphorbol 13-acetate (TPA) [Tsuchiya et al, 1982;Matikainen and Hurme, 1994]. In particular, IFN-g induces the expression of HLA-DR, FCreceptors, and CD54 (intercellular adhesion molecule-1) on THP-1 cell surface and enhances their anti-microbial, tumoricidal, and antigenpresenting capacities [Basham and Merigan, 1983;Gaffney et al, 1988].…”

mentioning

confidence: 99%

“…The triggering stimuli include interferon-gamma (IFN-g) [Gaffney et al, 1988], 1a,25 dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) [Vey et al, 1992], as well as pharmacological agents such as retinoic acid and 12-O-tetradecanoylphorbol 13-acetate (TPA) [Tsuchiya et al, 1982;Matikainen and Hurme, 1994]. In particular, IFN-g induces the expression of HLA-DR, FCreceptors, and CD54 (intercellular adhesion molecule-1) on THP-1 cell surface and enhances their anti-microbial, tumoricidal, and antigenpresenting capacities [Basham and Merigan, 1983;Gaffney et al, 1988]. The active form of vitamin D 3 , 1,25(OH) 2 D 3 , has been reported to induce myeloid differentiation of human mononuclear cells [Munker et al, 1986], and to affect metalloproteinase expression in THP-1 cells [Lacraz et al, 1994].…”

mentioning

confidence: 99%

Phorbol diester 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) up‐regulates the expression of estrogen receptors in human THP‐1 leukemia cells

Cutolo

¹

,

Carruba

²

,

Villaggio

³

et al. 2001

J of Cellular Biochemistry

View full text Add to dashboard Cite

In the present work, we have inspected expression of estrogen receptors (ER) and their regulation by the phorbol diester 12-O-tetradecanoylphorbol 13-acetate (TPA) in a leukemic cell line, the THP-1 cells, using multiple experimental approaches. Firstly, ligand binding assay (LBA) revealed that control (unstimulated) THP-1 cells express type I (high affinity, limited capacity) ER in the nuclear fraction only, whilst treatment of cells with TPA resulted in the appearance of type I ER in the soluble fraction as well, with the 50 ng/ml dose and the 48 h incubation time being the most effective experimental condition. A concomitant increase of type II ER was also seen in both soluble and nuclear cell fractions. Unstimulated THP-1 cells were found to be ER negative by immunocytochemistry; conversely, cells exposed to 50 ng/ml TPA for 48 h stained positively for ER, with the majority of cells having a strong nuclear staining. Scrutiny of ER mRNA expression using reverse transcriptase-polymerase chain reaction showed the presence of a wild type ER transcript in both control and TPA-treated THP-1 cells, though levels of ER mRNA were found to be comparatively higher in the latter. This combined evidence would imply that the TPA-induced differentiation of THP-1 cells is accompanied by the rise of high affinity (type I) ER, suggesting that estrogens may play a role in the regulation of macrophage activity during the inflammatory and/or the immune response.

show abstract

“…Gamma interferon (IFN-␥) is a key monocyte-activating cytokine (3,17,20,27). With IFN-␥ stimulation, the human monocytic cell line THP-1 expresses a number of genes associated with the antigen-presenting function of macrophages, including major histocompatibility complex class II (MHC-II), CD74 (MHC-II-associated invariant chain), and interleukin-1␤ (7,26; our unpublished observation). To identify genes involved in the function of macrophages, we had previously carried out PCR-based cDNA subtraction and subsequent differential display on mRNA obtained from IFN-␥-treated and untreated THP-1 cells.…”

mentioning

confidence: 86%

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Senju

¹

,

Iyama²,

Kaneko

³

et al. 2000

View full text Add to dashboard Cite

We previously identified a gene encoding a putative GTPase, GTPBP1, which is structurally related to elongation factor 1␣, a key component of protein biosynthesis machinery. The primary structure of GTPBP1 is highly conserved between human and mouse (97% identical at the amino acid level). Expression of this gene is enhanced by gamma interferon in a monocytic cell line, THP-1. Although counterparts of this molecule in Caenorhabditis elegans and Ascaris suum have also been identified, the function of this molecule remains to be clarified. In the present study, our immunohistochemical analyses on mouse tissues revealed that GTPBP1 is expressed in some neurons and smooth muscle cells of various organs as well as macrophages. Immunofluorescence analyses revealed that GTPBP1 is localized exclusively in cytoplasm and shows a diffuse granular network forming a gradient from the nucleus to the periphery of the cells in smooth muscle cell lines and macrophages. To investigate the physiological role of GTPBP1, we used targeted gene disruption in embryonic stem cells to generate GTPBP1-deficient mice. The mutant mice were born at the expected Mendelian frequency, developed normally, and were fertile. No manifest anatomical or behavioral abnormality was observed in the mutant mice. Functions of macrophages, including chemotaxis, phagocytosis, and nitric oxide production, in mutant mice were equivalent to those seen in wild-type mice. No significant difference was observed in the immune response to protein antigen between mutant mice and wild-type mice, suggesting normal function of antigen-presenting cells of the mutant mice. The absence of an eminent phenotype in GTPBP1-deficient mice may be due to functional compensation by GTPBP2, a molecule we recently identified which is similar to GTPBP1 in structure and tissue distribution.

show abstract

Regulation by Interferon γ of Function in the Acute Monocytic Leukemia Cell Line, THP-1

Cited by 15 publications

References 14 publications

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Phorbol diester 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) up‐regulates the expression of estrogen receptors in human THP‐1 leukemia cells

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Contact Info

Product

Resources

About