Regulation and stimulation of photosynthesis of mixotrophically cultured Haematococcus pluvialis by ribose

Pang, Na; Xie, Yao Jie; Oung, Hui Min Olivia; Sonawane, Balasaheb V.; Xiao, Fu; Kirchhoff, Helmut; Chen, Shulin

doi:10.1016/j.algal.2019.101443

Cited by 24 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acetate, a commonly used C-source for algae, was lowering µ max by 40%. Ribose, xylose and glycerol were not metabolized by the cells, despite reports showing consumption by other green algal species [ 6 , 33 , 34 ]. Therefore, subsequent experiments were done with either (i) glucose addition for highest biomass growth rates, or (ii) acetate supply as the most common C-source for carotenoid production, and (iii) an autotrophic control.…”

Section: Discussionmentioning

confidence: 88%

Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

et al. 2022

View full text Add to dashboard Cite

A novel strain of Coelastrella terrestris (Chlorophyta) was collected from red mucilage in a glacier foreland in Iceland. Its morphology showed characteristic single, ellipsoidal cells with apical wart-like wall thickenings. Physiological characterization revealed the presence of the rare keto-carotenoid adonixanthin, as well as high levels of unsaturated fatty acids of up to 85%. Initial screening experiments with different carbon sources for accelerated mixotrophic biomass growth were done. Consequently, a scale up to 1.25 L stirred photobioreactor cultivations yielded a maximum of 1.96 mg·L−1 adonixanthin in free and esterified forms. It could be shown that supplementing acetate to the medium increased the volumetric productivity after entering the nitrogen limitation phase compared to autotrophic control cultures. This study describes a promising way of biotechnological adonixanthin production using Coelastrella terrestris.

show abstract

Section: Discussionmentioning

confidence: 88%

Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The measured F v / F m values of both phototrophic and mixotrophic continuous cultures were around 0.7 throughout the cultivation period, and the photosynthetic efficiency was not affected by various levels of nitrogen in our experimental settings. Previously, Pang et al (2019) had reported that the mixotrophic cells of Haematococcus also showed a healthy status and highly efficient PSII activity. Although the cells under mixotrophic conditions showed decreased F v /F m values for the first 2 days, it seemed that the photosynthetic ability of cells recovered after 5 days of cultivation.…”

Section: Resultsmentioning

confidence: 99%

Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris

Khazi

Shi²,

Liaqat

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Although Haematococcus lacustris has been developed for astaxanthin production for decades, the production cost is still high. In order to modify the production processes, we proposed a novel strategy of cultivation, featured by sequential indoor continuous mixotrophic cultivation for the production of green cells followed by outdoor phototrophic induction for astaxanthin accumulation. The continuous mixotrophic cultivation was first optimized indoor, and then the seed culture of mixotrophic cultivation was inoculated into outdoor open raceway ponds for photoinduction. The results showed that mixotrophically grown cultures could efficiently grow without losing their photosynthetic efficiency and yielded higher biomass concentration (0.655 g L−1) and astaxanthin content (2.2% DW), compared to phototrophically grown seed culture controls. This novel strategy might be a promising alternative to the current approaches to advance the production technology of astaxanthin from microalgae.

show abstract

“…However, light is an important factor for Chlorella sp. to enhance growth and bioproduct synthesis as the energy source and it has been shown that photosynthesis plays a major role in producing energy in microalgae. , Dilution is helpful to alleviate the energy shortage from light penetration limitation for algae in dark wastewater . However, this increases treatment cost and uses of fresh water.…”

Section: Resultsmentioning

confidence: 99%

“…Potential microalgae were isolated from the dairy AD wastewater. One mL of the AD effluent lagoon samples were added to a 250 mL Erlenmeyer flask containing 100 mL BG-11 media prepared as previously described. , All cultures were incubated at 25 °C if not otherwise provided. Illumination was provided by cool-white fluorescent lamps.…”

Section: Methodsmentioning

confidence: 99%

Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance

Pang

Bergeron

et al. 2020

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

This study explored the possibility of incorporating extremophilic algal cultivation into dairy wastewater treatment by characterizing a unique algal strain. Results showed that extremophilic microalgae Chlorella vulgaris CA1 newly isolated from dairy wastewater tolerated a high level of ammonia nitrogen (2.7 g/L), which was over 20 times the ammonia nitrogen that regular Chlorella sp. could tolerate. The isolate was mixotrophically cultured in dairy effluent treated by anaerobic digestion (AD) for recycling nutrients and polishing the wastewater. The highest biomass content of 13.3 g/L and protein content of 43.4% were achieved in the culture in AD effluent. Up to 96% of the total nitrogen and 79% of the total phosphorus were removed from the dairy AD effluent. The ability of the algae to tolerate a high level of ammonia nitrogen suggests the potential for direct nutrient recycling from dairy wastewater while producing algal biomass and high value bioproducts.

show abstract

Regulation and stimulation of photosynthesis of mixotrophically cultured Haematococcus pluvialis by ribose

Cited by 24 publications

References 31 publications

Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris

Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance

Contact Info

Product

Resources

About