Regulation and Interactions of MT1-MMP and MMP-20 in Human Odontoblasts and Pulp Tissue in vitro

Abstract

MT1-MMP is a cell-membrane-bound metalloenzyme that activates other proMMPs such as proMMP-2 and -13. We studied MT1-MMP expression in mature human odontoblasts and pulp tissue, the regulation of MT1-MMP expression by growth factors TGF-beta1 and BMP-2, and the activation of odontoblast-derived MMP-20 by MT1-MMP. MT1-MMP mRNA is expressed by native and cultured mature human odontoblasts and pulp tissue. Western blot analysis of human odontoblasts and pulp tissue detects 65- and 51-kDa pro- and active forms of MT1-MMP, and smaller truncated MT1-MMP forms. BMP-2 down-regulates MT1-MMP expression in odontoblasts and pulp tissue, while TGF-beta1, alone or with BMP-2, decreases MT1-MMP mRNA levels only slightly. We also demonstrate that MT1-MMP is capable of converting proMMP-20 into a form corresponding to the active MMP-20. In conclusion, this study demonstrates the expression and differential regulation of MT1-MMP in human dentin-pulp complex cells, and the activation of MMP-20 by MT1-MMP.

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

“…We assume that EMMPRIN alone does not appear to regulate the production of MMP in ameloblasts or odontoblasts. In human odontoblasts and pulp tissue, MT1-MMP is constitutively expressed and downregulated by TGF and BMP-2 (Palosaari et al 2002). The stronger EMM-PRIN expression in the odontoblastic layer compared to that in undiVerentiated cells of the dental papilla is probably related to the cellular diVerentiation programme in the developing tooth germ.…”

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

“…MMP-8, MMP-2, MMP-9, MMP-3, MMP-2, MMP-14 and MMP-20 are the main MMPs that have been identified in pulp, odontoblasts and predentine/dentine. [8][9][10][11][12][13] MMP-13 has been found to be present in radicular dentine, which was expressed differently as caries progressed, but it was not found to have been expressed in the coronal dentine. 14 The immunoexpression of MMP-13 has been found to be weak and confined to the peritubular area in sound dentine and strong at the peritubular level and in Tome's process in dentine caries, where it decreased as the distance from the decay process increased.…”

“…19 Odontoblasts secrete at least gelatinase A (MMP-2), gelatinase B (MMP-9), collagenase 2 (MMP-8), collagenase-3 (MMP-13), enamelysin (MMP-20) and membrane type matrix metalloproteinase-1 (MT1-MMP). 8 Possible role of MMPs in mature odontoblasts could be -(i) physiological secondary dentine formation and mineralization in intact and healthy teeth, (ii) matrix degradation during dental injury, (iii) role in tertiary dentinogenesis and (iv) role in pulp inflammation. 30 Tumour growth factor (TGF) can upregulate MMP-9 expression and downregulate MMP-2 synthesis 31 but has no effect on collagen synthesis in odontoblasts.…”

The Role of Host‐derived Dentinal Matrix Metalloproteinases in Reducing Dentin Bonding of Resin Adhesives

“…The odontoblasts in developed human teeth synthesize and release several MMPs, including collagenase-2 (MMP-8) (Palosaari et al, 2000), gelatinases (MMP-2 and -9) (Tjäderhane et al, 1998b), membrane-bound MMP-14 (MT1-MMP) (Palosaari et al, 2002), enamelysin (MMP-20) (Sulkala et al, 2002) and cathepsin D (Linde and Persliden, 1977). The latent and active forms of gelatinolytic species (e.g.…”