Regulation and function of SOX9 during cartilage development and regeneration

Song, Haengseok; Park, Keun-Hong

doi:10.1016/j.semcancer.2020.04.008

Cited by 123 publications

(86 citation statements)

References 184 publications

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“…Polymer based systems, including poly L-lysine (PLL), polyethylenimine (PEI) or polyethylene glycol (PEG) are also of great interest for gene delivery into cells. These polymers form strong electrostatic complexes with nucleic acids, which facilitates their entry into cells (Raisin et al, 2016;Song and Park, 2020). Polymer complexes with hyaluronic acid (HA) enhance more efficient gene delivery, as HA binds to the MSC CD44 receptor, enabling complexes to enter cells.…”

Section: Non-viral Gene Transfer For Cartilage Repair In Oamentioning

confidence: 99%

“…Polymer complexes with hyaluronic acid (HA) enhance more efficient gene delivery, as HA binds to the MSC CD44 receptor, enabling complexes to enter cells. Such methods were used for delivering SOX genes into MSCs, strongly stimulating their chondrogenic differentiation (Song and Park, 2020). Similar gene carriers using chitosan-graft-PEI (CP)/DNA nanoparticles (Lu et al, 2014), as well as HA-chitosan modified systems (Lu et al, 2011) have been proposed as an efficient way for delivering the genes to chondrocytes and synoviocytes.…”

Section: Non-viral Gene Transfer For Cartilage Repair In Oamentioning

confidence: 99%

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Non-viral Gene Therapy for Osteoarthritis

Uzielienè

Kalvaityte

Bernotienė

et al. 2021

Front. Bioeng. Biotechnol.

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Strategies for delivering nucleic acids into damaged and diseased tissues have been divided into two major areas: viral and non-viral gene therapy. In this mini-review article we discuss the application of gene therapy for the treatment of osteoarthritis (OA), one of the most common forms of arthritis. We focus primarily on non-viral gene therapy and cell therapy. We briefly discuss the advantages and disadvantages of viral and non-viral gene therapy and review the nucleic acid transfer systems that have been used for gene delivery into articular chondrocytes in cartilage from the synovial joint. Although viral gene delivery has been more popular due to its reported efficiency, significant effort has gone into enhancing the transfection efficiency of non-viral delivery, making non-viral approaches promising tools for further application in basic, translational and clinical studies on OA. Non-viral gene delivery technologies have the potential to transform the future development of disease-modifying therapeutics for OA and related osteoarticular disorders. However, further research is needed to optimize transfection efficiency, longevity and duration of gene expression.

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Section: Non-viral Gene Transfer For Cartilage Repair In Oamentioning

confidence: 99%

Section: Non-viral Gene Transfer For Cartilage Repair In Oamentioning

confidence: 99%

Non-viral Gene Therapy for Osteoarthritis

Uzielienè

Kalvaityte

Bernotienė

et al. 2021

Front. Bioeng. Biotechnol.

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show abstract

“…These compounds exert their effects by stimulating differentiation. SOX9 appears to be a common target in the modulation of the dedifferentiated phenotype [ 36 , 38 , 41 ], functioning as a master transcription factor determining chondrocyte differentiation and phenotype [ 53 ]. However, the main molecular events involved in chondrocyte expansion and dedifferentiation are still unclear, making it difficult to efficiently regulate the chondrocyte phenotype in long-term passaging.…”

Section: Generating Chondrocytes Using Small Moleculesmentioning

confidence: 99%

Pharmaceutical therapeutics for articular regeneration and restoration: state-of-the-art technology for screening small molecular drugs

Chen

Yao

et al. 2021

Cell. Mol. Life Sci.

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“…Here, mainly anti-inflammatory genes like IL-1, IL-6, and IL-10 were influenced by vector transfer. A prominent inducer for the regeneration of cartilage, i.e., SOX9, delivered in non-viral approach has been shown as a promising strategy (Song and Park, 2020). Also, here a wide range of studies used liposome-based methods to transfect primary chondrocytes and MSCs (Goomer et al, 2001;Stöve et al, 2002;Sun et al, 2009).…”

Section: Lipid-based Gene Vectors For Cartilage Repairmentioning

confidence: 99%

Non-viral Gene Delivery Methods for Bone and Joints

Gantenbein

Tang

Guerrero

et al. 2020

Front. Bioeng. Biotechnol.

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Viral carrier transport efficiency of gene delivery is high, depending on the type of vector. However, viral delivery poses significant safety concerns such as inefficient/unpredictable reprogramming outcomes, genomic integration, as well as unwarranted immune responses and toxicity. Thus, non-viral gene delivery methods are more feasible for translation as these allow safer delivery of genes and can modulate gene expression transiently both in vivo, ex vivo, and in vitro. Based on current studies, the efficiency of these technologies appears to be more limited, but they are appealing for clinical translation. This review presents a summary of recent advancements in orthopedics, where primarily bone and joints from the musculoskeletal apparatus were targeted. In connective tissues, which are known to have a poor healing capacity, and have a relatively low cell-density, i.e., articular cartilage, bone, and the intervertebral disk (IVD) several approaches have recently been undertaken. We provide a brief overview of the existing technologies, using nano-spheres/engineered vesicles, lipofection, and in vivo electroporation. Here, delivery for microRNA (miRNA), and silencing RNA (siRNA) and DNA plasmids will be discussed. Recent studies will be summarized that aimed to improve regeneration of these tissues, involving the delivery of bone morphogenic proteins (BMPs), such as BMP2 for improvement of bone healing. For articular cartilage/osteochondral junction, non-viral methods concentrate on targeted delivery to chondrocytes or MSCs for tissue engineering-based approaches. For the IVD, growth factors such as GDF5 or GDF6 or developmental transcription factors such as Brachyury or FOXF1 seem to be of high clinical interest. However, the most efficient method of gene transfer is still elusive, as several preclinical studies have reported many different non-viral methods and clinical translation of these techniques still needs to be validated. Here we discuss the non-viral methods applied for bone and joint and propose methods that can be promising in clinical use.

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Regulation and function of SOX9 during cartilage development and regeneration

Cited by 123 publications

References 184 publications

Non-viral Gene Therapy for Osteoarthritis

Non-viral Gene Therapy for Osteoarthritis

Pharmaceutical therapeutics for articular regeneration and restoration: state-of-the-art technology for screening small molecular drugs

Non-viral Gene Delivery Methods for Bone and Joints

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