Regulation and function of <i>Dbx</i> genes in the zebrafish spinal cord

Gribble, Suzanna L.; Nikolaus, O. Brant; Dorsky, Richard I.

doi:10.1002/dvdy.21367

Cited by 42 publications

(56 citation statements)

References 47 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, all post-mitotic neurons express HuC/D which is detectable with immunocytochemistry 1,5 . mRNA probes for islet, gata, and vsx families label specific post-mitotic neurons of consistent position and number within each spinal cord hemisegment 6 .…”

Section: Representative Resultsmentioning

confidence: 99%

“…Aided by the optical clarity of the embryonic zebrafish, the ability to perform forward genetic screens, reverse genetics, and transgenic approaches, several developmental processes may be investigated. This includes mitotic indices of neuronal progenitors with markers like BrdU and anti-phospho-histone 3 [4][5][6] and patterning through expression of neuronal and glial progenitor markers in the pax, nkx, dbx, and olig families 1,[4][5][6] . Reagents that report glial and neuronal determination (such as glial fibrillary acid protein (GFAP) and HuC/D) 1,4,5,10 can be used.…”

Section: Discussionmentioning

confidence: 99%

“…This includes mitotic indices of neuronal progenitors with markers like BrdU and anti-phospho-histone 3 [4][5][6] and patterning through expression of neuronal and glial progenitor markers in the pax, nkx, dbx, and olig families 1,[4][5][6] . Reagents that report glial and neuronal determination (such as glial fibrillary acid protein (GFAP) and HuC/D) 1,4,5,10 can be used. Neuronal subtype differentiation can be analyzed through expression of islet, vsx, engrailed, and gata genes 1,[4][5][6] .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, debris from damaged yolk prohibits clear visualization of tissues. Though cross sections in the dorso-ventral (DV) axis are possible, they do not readily permit simultaneous visualization in the anterior-posterior (AP) axis 1 …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Beck

Watt

Bonner

2014

JoVE

View full text Add to dashboard Cite

The zebrafish spinal cord is an effective investigative model for nervous system research for several reasons. First, genetic, transgenic and gene knockdown approaches can be utilized to examine the molecular mechanisms underlying nervous system development. Second, large clutches of developmentally synchronized embryos provide large experimental sample sizes. Third, the optical clarity of the zebrafish embryo permits researchers to visualize progenitor, glial, and neuronal populations. Although zebrafish embryos are transparent, specimen thickness can impede effective microscopic visualization. One reason for this is the tandem development of the spinal cord and overlying somite tissue. Another reason is the large yolk ball, which is still present during periods of early neurogenesis. In this article, we demonstrate microdissection and removal of the yolk in fixed embryos, which allows microscopic visualization while preserving surrounding somite tissue. We also demonstrate semipermanent mounting of zebrafish embryos. This permits observation of neurodevelopment in the dorso-ventral and anterior-posterior axes, as it preserves the three-dimensionality of the tissue.

show abstract

Section: Representative Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Beck

Watt

Bonner

2014

JoVE

View full text Add to dashboard Cite

show abstract

“…Therefore, we reasoned that we could use this line to FAC-sort GFPlabelled CiAs and test whether we could obtain RNA from these cells of sufficient quality and quantity for expression-profiling. As a further proof-of-principle for FAC-sorting and RNA-profiling fluorescent-protein-labelled zebrafish neurons and as a comparison population for the Tg(pax2a:GFP) samples, we decided to FAC-sort EGFP-labelled neurons from Tg(elav13:EGFP) embryos, in which at least most post-mitotic neurons express EGFP (Park et al, 2000;Gribble et al, 2007). Finally, as a control and further comparison population, we also FACsorted all trunk cells.…”

Section: Introductionmentioning

confidence: 99%

RNA profiling of FAC‐sorted neurons from the developing zebrafish spinal cord

Cerda

Hargrave

Lewis

2008

Developmental Dynamics

View full text Add to dashboard Cite

In this report, we describe a successful protocol for isolating and expression-profiling live fluorescentprotein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in general and to all trunk cells. We show that RNA of sufficient quality and quantity to uncover both expected and novel transcription profiles via Affymetrix microarray analysis can be extracted from 5,700 to 20,000 FAC-sorted cells. As part of this study, we also further confirm the genetic homology of mammalian and zebrafish V1 interneurons, by demonstrating that zebrafish V1 cells (CiAs) express genes that encode for the transcription factors Lhx1a and Lhx5. This protocol for dissociating, sorting and RNA-profiling neurons from organogenesis-stage zebrafish embryos should also be applicable to other developing organs and tissues and potentially other model organisms. Developmental Dynamics 238:150 -161, 2009.

show abstract

Diversity within olfactory sensory derivatives revealed by the contribution of Dbx1 lineages

Causeret

Fayon

Moreau

et al. 2023

J of Comparative Neurology

View full text Add to dashboard Cite

In vertebrates, the embryonic olfactory epithelium contains progenitors that will give rise to distinct classes of neurons, including olfactory sensory neurons (OSNs; involved in odor detection), vomeronasal sensory neurons (VSNs; responsible for pheromone sensing), and gonadotropin-releasing hormone (GnRH) neurons that control the hypothalamic-pituitary-gonadal axis. Currently, these three neuronal lineages are usually believed to emerge from uniform pools of progenitors. Here, we found that the homeodomain transcription factor Dbx1 is expressed by neurogenic progenitors in the developing and adult mouse olfactory epithelium. We demonstrate that Dbx1 itself is dispensable for neuronal fate specification and global organization of the olfactory sensory system. Using lineage tracing, we characterize the contribution of Dbx1 lineages to OSN, VSN, and GnRH neuron populations and reveal an unexpected degree of diversity. Furthermore, we demonstrate that Dbx1-expressing progenitors remain neurogenic in the absence of the proneural gene Ascl1. Our work therefore points to the existence of distinct neurogenic programs in Dbx1-derived and other olfactory lineages.

show abstract

Regulation and function of Dbx genes in the zebrafish spinal cord

Cited by 42 publications

References 47 publications

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

RNA profiling of FAC‐sorted neurons from the developing zebrafish spinal cord

Diversity within olfactory sensory derivatives revealed by the contribution of Dbx1 lineages

Contact Info

Product

Resources

About