Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements

Melo, Bruno Paes de; Moura, Stéfanie Menezes de; Morgante, Carolina Vianna; Pinheiro, Daniele H.; Alves, Nayara Sabrina F.; Rodrigues-Silva, Paolo Lucas; Lourenço-Tessutti, Isabela Tristan; Andrade, Rosângela Vieira de; Fragoso, Rodrigo da Rocha; Grossi-de-Sá, Maria Fátima

doi:10.4322/biori.202105

Cited by 7 publications

(2 citation statements)

References 106 publications

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“…Understanding cis-acting elements in gene promoters, like G-box and ABRE, is crucial for unravelling plant stress responses. The abundance of cis-elements suggests that these genes may be triggered in response to various environmental stresses, potentially improving its overall stress tolerance [63].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of heavy-metal ATPases genes in Areca catechu: investigating their functionality under heavy metal exposure

Khan,

Ali,

Wan

et al. 2024

BMC Plant Biol

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Heavy-metal ATPases (HMAs) play a vital role in plants, helping to transport heavy metal ions across cell membranes.However, insufficient data exists concerning HMAs genes within the Arecaceae family.In this study, 12 AcHMA genes were identified within the genome of Areca catechu, grouped into two main clusters based on their phylogenetic relationships.Genomic distribution analysis reveals that the AcHMA genes were unevenly distributed across six chromosomes. We further analyzed their physicochemical properties, collinearity, and gene structure.Furthermore, RNA-seq data analysis exhibited varied expressions in different tissues of A. catechu and found that AcHMA1, AcHMA2, and AcHMA7 were highly expressed in roots, leaves, pericarp, and male/female flowers. A total of six AcHMA candidate genes were selected based on gene expression patterns, and their expression in the roots and leaves was determined using RT-qPCR under heavy metal stress. Results showed that the expression levels of AcHMA1 and AcHMA3 genes were significantly up-regulated under Cd2 + and Zn2 + stress. Similarly, in response to Cu2+, the AcHMA5 and AcHMA8 revealed the highest expression in roots and leaves, respectively. In conclusion, this study will offer a foundation for exploring the role of the HMAs gene family in dealing with heavy metal stress conditions in A. catechu.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of heavy-metal ATPases genes in Areca catechu: investigating their functionality under heavy metal exposure

Khan,

Ali,

Wan

et al. 2024

BMC Plant Biol

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“…Despite the knowledge of the core photorespiratory enzymes very little is known about how they are regulated ( Rojas et al, 2012 ; Saji et al, 2017 ). Recent reports have shown the potential of utilizing cis -regulatory elements (CREs) of endogenous genes to fine-tune transgene expression for preserving spatiotemporal regulatory characteristics ( Kaur et al, 2017 ; Cai et al, 2020 ; Jores et al, 2021 ; Kakei et al, 2021 ; Melo et al, 2021 ). Synthetic promoters designed with single or multiple CREs identified from native photorespiratory genes can provide valuable insights into the potential positive and negative regulators of photorespiration.…”

Section: Introductionmentioning

confidence: 99%

Design and Analysis of Native Photorespiration Gene Motifs of Promoter Untranslated Region Combinations Under Short Term Abiotic Stress Conditions

Basu

South

2022

Front. Plant Sci.

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Quantitative traits are rarely controlled by a single gene, thereby making multi-gene transformation an indispensable component of modern synthetic biology approaches. However, the shortage of unique gene regulatory elements (GREs) for the robust simultaneous expression of multiple nuclear transgenes is a major bottleneck that impedes the engineering of complex pathways in plants. In this study, we compared the transcriptional efficacies of a comprehensive list of well-documented promoter and untranslated region (UTR) sequences side by side. The strength of GREs was examined by a dual-luciferase assay in conjunction with transient expression in tobacco. In addition, we created suites of new GREs with higher transcriptional efficacies by combining the best performing promoter-UTR sequences. We also tested the impact of elevated temperature and high irradiance on the effectiveness of these GREs. While constitutive promoters ensure robust expression of transgenes, they lack spatiotemporal regulations exhibited by native promoters. Here, we present a proof-of-principle study on the characterization of synthetic promoters based on cis-regulatory elements of three key photorespiratory genes. This conserved biochemical process normally increases under elevated temperature, low CO2, and high irradiance stress conditions and results in ∼25% loss in fixed CO2. To select stress-responsive cis-regulatory elements involved in photorespiration, we analyzed promoters of two chloroplast transporters (AtPLGG1 and AtBASS6) and a key plastidial enzyme, AtPGLP using PlantPAN3.0 and AthaMap. Our results suggest that these motifs play a critical role for PLGG1, BASS6, and PGLP in mediating response to elevated temperature and high-intensity light stress. These findings will not only enable the advancement of metabolic and genetic engineering of photorespiration but will also be instrumental in related synthetic biology approaches.

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Discovery and functional characterization of novel cotton promoters with potential application to pest control

et al. 2022

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Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements

Cited by 7 publications

References 106 publications

Genome-wide identification of heavy-metal ATPases genes in Areca catechu: investigating their functionality under heavy metal exposure

Genome-wide identification of heavy-metal ATPases genes in Areca catechu: investigating their functionality under heavy metal exposure

Design and Analysis of Native Photorespiration Gene Motifs of Promoter Untranslated Region Combinations Under Short Term Abiotic Stress Conditions

Discovery and functional characterization of novel cotton promoters with potential application to pest control

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