Regulated extravascular microenvironment via reversible thermosensitive hydrogel for inhibiting calcium influx and vasospasm

Zhao, Binfan; Zhuang, Yaping; Liu, Zhimo; Mao, Jiayi; Qian, Shutong; Zhao, Qing; Lu, Bolun; Mao, Xiyuan; Zhang, Liucheng; Zhang, Yuguang; Cui, Wenguo; Sun, Xiaoming

doi:10.1016/j.bioactmat.2022.08.024

Cited by 9 publications

(9 citation statements)

References 69 publications

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“…The vial inversion method studied the sol-gel phase transition behavior and phase transition time of PPH thermosensitive hydrogels [ 40 ]. In total, 2 mL of PPH thermosensitive hydrogels were added to 5 mL of filling vials, stored at 4 °C for 10 min, and then placed in a preheated 37 °C constant temperature water bath and inverted after every 15 s to check the sol-gel phase transition.…”

Section: Methodsmentioning

confidence: 99%

The Controlled Release and Prevention of Abdominal Adhesion of Tannic Acid and Mitomycin C-Loaded Thermosensitive Gel

Liu

Wang

et al. 2023

Polymers

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Postoperative abdominal adhesion is one of the most common complications after abdominal surgery. A single drug or physical barrier treatment does not achieve the ideal anti-adhesion effect. We developed a thermosensitive hydrogel (PPH hydrogel) consisting of poloxamer 407 (P407), poloxamer (P188), and hydroxypropyl methylcellulose (HPMC) co-blended. An injectable thermosensitive TA/MMC-PPH hydrogel was obtained by loading tannic acid (TA) with an anti-inflammatory effect and mitomycin C (MMC), which inhibits fibroblast migration or proliferation. The optimal prescriptions of PPH hydrogels with a suitable gelling time (63 s) at 37 °C was 20% (w/v) P407, 18% (w/v) P188, and 0.5% (w/v) HPMC. The scanning electron microscopy (SEM) revealed that the PPH hydrogel had a three-dimensional mesh structure, which was favorable for drug encapsulation. The PPH hydrogel had a suitable gelation temperature of 33 °C, a high gel strength, and complicated viscosity at 37 °C, according to the rheological analysis. In vitro release studies have shown that the PPH hydrogel could delay the release of TA and MMC and conform to the first-order release rate. Anti-adhesion tests performed on rats in vivo revealed that TA/MMC-PPH hydrogel significantly reduced the risk of postoperative adhesion. In conclusion, the TA/MMC-PPH hydrogel prepared in this study showed an excellent performance in both controlled drug release and anti-adhesive effects. It can be used as a protocol to prevent or reduce postoperative abdominal adhesion.

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Section: Methodsmentioning

confidence: 99%

The Controlled Release and Prevention of Abdominal Adhesion of Tannic Acid and Mitomycin C-Loaded Thermosensitive Gel

Liu

Wang

et al. 2023

Polymers

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“…Their spatiotemporal controllability and noninvasive manipulation of drug/gene/protein delivery promote the development of the corresponding stimuli-responsive DDSs. Specifically, based on the thermo-sensitive lipid molecules and chemical bonds, thermo-responsive GDNCs would be well designed [141][142][143]. The US can penetrate deep tissues with high precision and can control the release of bioactive molecules without causing harm to nearby healthy tissues [144].…”

Section: Other Stimulimentioning

confidence: 99%

Stimuli-Responsive Gene Delivery Nanocarriers for Cancer Therapy

Zhang

Kuang

et al. 2023

Nano-Micro Lett.

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Gene therapy provides a promising approach in treating cancers with high efficacy and selectivity and few adverse effects. Currently, the development of functional vectors with safety and effectiveness is the intense focus for improving the delivery of nucleic acid drugs for gene therapy. For this purpose, stimuli-responsive nanocarriers displayed strong potential in improving the overall efficiencies of gene therapy and reducing adverse effects via effective protection, prolonged blood circulation, specific tumor accumulation, and controlled release profile of nucleic acid drugs. Besides, synergistic therapy could be achieved when combined with other therapeutic regimens. This review summarizes recent advances in various stimuli-responsive nanocarriers for gene delivery. Particularly, the nanocarriers responding to endogenous stimuli including pH, reactive oxygen species, glutathione, and enzyme, etc., and exogenous stimuli including light, thermo, ultrasound, magnetic field, etc., are introduced. Finally, the future challenges and prospects of stimuli-responsive gene delivery nanocarriers toward potential clinical translation are well discussed. The major objective of this review is to present the biomedical potential of stimuli-responsive gene delivery nanocarriers for cancer therapy and provide guidance for developing novel nanoplatforms that are clinically applicable.

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“…[38,39] The ligand binds to the cytomembrane nucleolin receptor, which triggers a rapid and intense membrane Ca 2+ flux in various types of cells. [40,41] Therefore, the amplification of the Ca 2+ influx mediated by the polyvalent effect of GC-cholo-apt-cDNA and cytomembrane nucleolin is a critical issue. Fluorescence imaging was first carried out to elucidate the potential apoptotic pathway.…”

Section: Investigation On the Apoptotic Pathwaymentioning

confidence: 99%

Multi‐Catcher Polymers Regulate the Nucleolin Cluster on the Cell Surface for Cancer Therapy

Cheng

Jiang

Kong

et al. 2023

Adv Healthcare Materials

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Cell signal transduction mediated by cell surface ligand‐receptor is crucial for regulating cell behavior. The oligomerization or hetero‐aggregation of the membrane receptor driven by the ligand realizes the rearrangement of apoptotic signals, providing a new ideal tool for tumor therapy. However, the construction of a stable model of cytomembrane receptor aggregation and the development of a universal anti‐tumor therapy model on the cellular surface remain challenging. This work describes the construction of a “multi‐catcher” flexible structure GC‐chol‐apt‐cDNA with a suitable integration of the oligonucleotide aptamer (apt) and cholesterol (chol) on a polymer skeleton glycol chitosan (GC), for the regulation of the nucleolin cluster through strong polyvalent binding and hydrophobic membrane anchoring on the cell surface. This oligonucleotide aptamer shows nearly 100‐fold higher affinity than that of the monovalent aptamer and achieves stable anchoring to the plasma membrane for up to 6 h. Moreover, it exerts a high tumor inhibition both in vitro and in vivo by activating endogenous mitochondrial apoptosis pathway through the cluster of nucleolins on the cell membrane. This multi‐catcher nano‐platform combines the spatial location regulation of cytomembrane receptors with the intracellular apoptotic signaling cascade and represents a promising strategy for antitumor therapy.

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Regulated extravascular microenvironment via reversible thermosensitive hydrogel for inhibiting calcium influx and vasospasm

Cited by 9 publications

References 69 publications

The Controlled Release and Prevention of Abdominal Adhesion of Tannic Acid and Mitomycin C-Loaded Thermosensitive Gel

The Controlled Release and Prevention of Abdominal Adhesion of Tannic Acid and Mitomycin C-Loaded Thermosensitive Gel

Stimuli-Responsive Gene Delivery Nanocarriers for Cancer Therapy

Multi‐Catcher Polymers Regulate the Nucleolin Cluster on the Cell Surface for Cancer Therapy

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