Regulated expression of the prolactin gene in rat pituitary tumor cells.

Brennessel, Barbara A.; Biswas, Debajit K.

doi:10.1083/jcb.87.1.6

Cited by 22 publications

(11 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PRL production in GH4C1, 928-9b, and FBGH12C, before (GH4C1 > 928-9b > FBGH12C) and after TRH treatment could be correlated to the levels ofnuclear mRNAPRL sequences in these cells. A similar relationship was observed when cytoplasmic mRNAPRL sequences were examined in an analogous study (1). The increase in the amounts of nuclear RNAPRL sequences in TRH-treated cells as determined by RNA cDNAPRL solution hybridization would have been difficult to interpret without the blot analysis because of the possible contamination of nuclei with cytoplasm.…”

Section: Nuclear and Cytoplasmic Rnaprl Sequences In Control Andmentioning

confidence: 53%

“…Previous studies have demonstrated a direct relationship between the amount of translatable mRNAPRL in control and TRH-or BrdUrd-treated cells and the levels of PRL production in each cell line (1,(4)(5)(6)(7). This direct relationship was also observed for the levels of hybridizable mRNAPRL sequences of control and TRH-treated cells (1,5). However, such studies did not show whether the variation in cytoplasmic mRNAPRL levels was a reflection ofnuclear events or ofalterations in cytoplasmic mRNAPRL turnover or stability.…”

Section: Nuclear and Cytoplasmic Rnaprl Sequences In Control Andmentioning

confidence: 78%

“…Isolation, growth conditions, and properties of the three substrains used in this investigation have been described in detail (1).…”

Section: Methodsmentioning

confidence: 99%

“…Difference in basal production. of PRL by these GH cell strains can be correlated with the amounts of cytoplasmic RNAPRL sequences detected by cell-free translational assay and by hybridization to cDNAPRL (DNA complementary to mRNAPRL) (1). PRL synthesis in PRL' GH4G1 cells can be further stimulated by treating the cells with thyrotropin-releasing hormone (TRH) (2,3).…”

mentioning

confidence: 99%

“…Prolactin (PRL) production in a hybrid strain (928-9b) of GH cells (rat pituitary tumor cells) has been found to be grossly different from that in the PRL' GH4C1 and PRL-FBGH12C1-parent strains from which it was isolated (1). Difference in basal production.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Mechanism of induction of prolactin synthesis in GH cells

Biswas

Hanes

Brennessel

1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Prolactin-specific RNA (RNApRL) in total nuclear RNA and in cytoplasmic poly(A)+RNA isolated from GH (rat pituitary) cells was selectively hybridized to immobilized cloned cDNApRL. Agarose gel electrophoresis. of the nuclear RNAPRL sequences eluted from the nitrocellulose filters revealed several RNA species of approximately 25-30, 18-19, and 12-13 S. Only the 12-13 S RNA species could be detected in the cytoplasmic poly(A)+RNA fraction. Comparative analysis oftotal nuclear RNA of control and thyrotropin-releasing hormone (thyroliberin)-treated cells by the reverse Southern blot technique demonstrated increased levels of all the nuclear RNAPRL species in hormone, treated cells. Nuclear and cytoplasmic RNAPRL sequences in control and treated cells were quantitated by molecular hybridization to clonedcDNApRL. The 2-to 34old stimulation ofPRL production by thyrotropin-releasing hormone-treated GH4C1 cells could be correlated to the. corresponding increase of nuclear RNAPRL sequences. The hybrid strain, which produces 1/5th the amount of PRL that the parent GH4Cj does, had 1/5th the amounts of nuclear RNAPRL sequences. Thyrotropin-releasing hormone affected neither prolactin production nor nuclear RNAPRL level in 928-9b cells. RNAPRL sequences could not be detected either in nuclei or in cytoplasm of prolactin nonproducing F1BGH12C, cells. However, prolactin production could be induced and RNAPRL sequences could be detected in the total nuclear.RNA and in cytoplasmic poly(A)+RNA fraction after treatment-of this GH cell substrain with 5-bromodeoxyuridine. These results demonstrate that differential basal prolactin production and 'its modulation by thyrotropin-releasing hormone and by 5-bromodeoxyuridine can be correlated to the altered levels'of nuclear RNAPRL sequences in the three GH cell strains.Prolactin (PRL) production in a hybrid strain (928-9b) (8). However, it has not~been known whether the increased PRL production results from increased production of nuclear and cytoplasmic RNAPRL sequences. This investigation also demonstrates the close correlation ofBrdUrd-induced PRL synthesis with a concomitant increase in nuclear and cytoplasmic RNAPRL sequences, from undetectable levels to detectable amounts. MATERIALS AND METHODSGH cells are clonal strains of rat pituitary tumor cells in culture (9). Isolation, growth conditions, and properties of the three substrains used in this investigation have been described in detail (1).Isolation of Nuclear and Cytoplasmic RNA. Isolation of nuclei and cytoplasm and of total RNA and poly(A)+RNA from these subcellular fractions has been described (10,11 by insertion of cDNAPRL (approximately 900 base pairs) into plasmid pBR322 at the Pst I site.

show abstract

Section: Nuclear and Cytoplasmic Rnaprl Sequences In Control Andmentioning

confidence: 53%

Section: Nuclear and Cytoplasmic Rnaprl Sequences In Control Andmentioning

confidence: 78%

“…Isolation, growth conditions, and properties of the three substrains used in this investigation have been described in detail (1).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Mechanism of induction of prolactin synthesis in GH cells

Biswas

Hanes

Brennessel

1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

Oncogene homologues in yeast

Wheals

1985

BioEssays

View full text Add to dashboard Cite

Two different yeasts have a number of genes bearing striking structural and functional homologies to mammalian oncogenes. In yeast these genes are involved in the control of proliferation and early steps in the cell cycle. Many have putative protein kinase activity and some have been shown to control the activity of the enzyme adenylate cyclase which synthesizes cyclic AMP. Mutant forms of these yeast genes have oncogenic activity in mammalian cells.

show abstract

Rat serum albumin synthesis in variant rat hepatoma cells

Wolf

Golden

Chasin

1983

Journal Cellular Physiology

View full text Add to dashboard Cite

Variant subclones of the rat hepatoma cell line FU5-5 have been isolated that are altered in their production of rat serum albumin. Three of these variants, isolated in a random screening, have been categorized as high, intermediate, and low producers. They secrete albumin into the culture medium at different rates: 16, 1.7, and 0.3 microgram/mg cell protein/48 h. A fourth variant, isolated on the basis of altered morphology, secretes no detectable albumin. Unlike the albumin-producing variants, this null variant is also deficient in the level and inducibility of tyrosine aminotransferase activity. Albumin biosynthesis as determined in pulse-labeling experiments is affected similarly in the four variants, yielding albumin synthetic rates of 0.24, 0.035, 0.006, and less than 0.002% of total protein synthesis. The translatable albumin messenger RNA content in these variants was measured using a rabbit reticulocyte lysate system. The null variant contains no detectable mRNA, and the three quantitative variants contain levels of translatable albumin messenger RNA corresponding to 0.07, 0.03, and 0.005% of total stimulated polypeptide synthesis. The highest producing variant contains less translatable albumin mRNA than expected on the basis of cellular biosynthetic measurements, suggesting a translation efficiency difference in this clone. Cell hybrids constructed by fusing the high-producing clone and the null variant produce little or no albumin. This extinction indicates that the null variant contains a diffusible regulatory factor capable of decreasing albumin gene expression. The relatively stable and discrete heritable phenotypic changes exhibited by these clones may serve as a model for similar changes that occur during hepatic differentiation.

show abstract

Regulated expression of the prolactin gene in rat pituitary tumor cells.

Cited by 22 publications

References 20 publications

Mechanism of induction of prolactin synthesis in GH cells

Mechanism of induction of prolactin synthesis in GH cells

Oncogene homologues in yeast

Rat serum albumin synthesis in variant rat hepatoma cells

Contact Info

Product

Resources

About