In this paper, the author studies the boundedness for a large class of sublinear operator Tα, α ∈ [0, n) generated by Calderón-Zygmund singular integral operators (α = 0) and generated by fractional integral potential operators (α > 0) on the generalized mixed Morrey spaces M ϕ q (R n ). Moreover, the boundeness for the commutators of Tα, α ∈ [0, n) on the generalized mixed Morrey spaces M ϕ q (R n ) is also studied. As applications, we obtain the boundedness for the Hardy-Littlewood maximal operator, the Calderón-Zygmund singular integral operator, the fractional integral operator, the fractional maximal operator and their commutators on the generalzied mixed Morrey spaces.