Regular swimming before the induction of ulcer ameliorates oxidative gastric damage in rats: The anxiolytic effect of exercise

Abstract

Objectives:To investigate the impact of exercise on ulcerogenesis. Materials and Methods:Gastric ulcer was induced in Wistar albino rats that were either kept sedentary or had swimming exercise for 6 weeks. Under anesthesia, ulcer was induced by applying acetic acid on the stomach serosa, while control rats had sham-operation. On 3rd or 10th days of ulcer induction, anxiety levels were measured and rats were decapitated for measuring serum cortisol level, while gastric tissue samples were obtained for histopathologic analysis and for determination of malondialdehyde (MDA), glutathione (GSH) levels, myeloperoxidase (MPO) activity, luminol-and lucigenin-enhanced chemiluminescence (CL) and nitric oxide (NO) levels. Results:In sedentary 3-day-ulcer group, gastric MPO activities were elevated with concomitant reductions in GSH content, while these changes were reversed in 10-day ulcer group. In exercised 3-day-ulcer group, all parameters were significantly reversed. Increased lucigenin-CL was suppressed, while NO levels were elevated by exercise. Histological analysis revealed lower lesion scores in exercised groups. Serum cortisol levels were not different among ulcer groups, but anxiety was suppressed by swimming exercise in 3-day-ulcer group. Gereç ve Yöntem:Wistar albino sıçanlar, sedanter ve egzersiz grupları olarak ikiye ayrıldı. Altı haftalık egzersizin sonunda, anestezi altında mide serozasına asetik-asit uygulanarak ülser oluşturulurken, kontrol gruplarına taklit-cerrahi yapıldı. Ülser uygulanmasının 3. ve 10. günlerinde, anksiyete düzeyleri belirlendi ve sıçanlar dekapite edildi. Serum kortizol seviyeleri ölçüldü; mide dokusu örnekleri malondialdehit (MDA), glutatyon (GSH), miyeloperoksidaz (MPO), nitrik oksit (NO), luminol-lusigenin probları ile kemiluminesans (KL) ölçümleri ve histopatolojik analiz için alındı. Bulgular: Kontrol gruplarına kıyasla sedanter 3 ve 10 günlük ülser gruplarında MPO ve MDA seviyeleri artarken, GSH seviyeleri azaldı. Bu değişiklikler 10 günlük ülser gruplarında kontrol düzeylerine döndü. Egzersiz yapan 3 günlük ülser grubunda tüm parametreler tersine döndü. Ülser ile artmış bulunan lusigenin KL düzeyi egzersiz ile baskılanırken NO düzeyi egzersizle anlamlı olarak artmış bulundu. Histolojik incelemede egzersiz gruplarında lezyon skorları düşük bulundu. Ülser yapılan sedanter ve egzersiz gruplarında kortizol seviyeleri arasında farklılık görülmedi. Ülsere bağlı artan anksiyete, 3 günlük egzersiz yapan ülser gruplarında azalırken 10 günlük ülser grubunda değişmedi.Sonuç: Düzenli yüzme egzersizi, dokuya nötrofil infiltrasyonunu engellemek, NO yapımını uyarmak ve anksiyeteyi azaltmak yoluyla midede oksidatif hasarı hafifletmektedir. Düzenli egzersiz, mide ülserinin gelişmesini yavaşlatan ve iyileşmesini kolaylaştıran bir yaklaşım olarak dikkate alınmalıdır.

Regular moderate exercise alleviates gastric oxidative damage in rats via the contribution of oxytocin receptors

“…Physical activity, which reduces the risk for developing chronic diseases (Alessio et al 2005;Beavers et al 2010), is also regarded as a non-pharmacological approach for reducing ulcer incidence in men (Cheng et al 2000a;Radak et al 2008). Prolonged moderate-intensity exercise, in contrast to acute and intensive exercise, was proven to reduce gastric mucosal damage and alleviate the stress response with reductions in the circulating levels of cortisol (Cheng et al 2000b;Bahadir et al 2016;Shephard, 2017). Although exercise was found to increase serum oxytocin levels (Irianti et al 2017) and peripheral administration of oxytocin was reported to exert an anti-ulcer activity (Asad et al 2001b), the role of oxytocin in the protective effect of exercise on gastric ulcer has not yet been evaluated.…”

“…A moderate level of exercise was shown to exert beneficial effects by fine-tuning ROS production (Peternelj & Coombes, 2011;Bouzid et al 2018) and by the activation of adaptive anti-oxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase, etc. ), which prepare the gastric mucosa for any upcoming injurious events (Navarro et al 2004;Ji et al 2006;Bahadir et al 2016;Simioni et al 2018). However, hormonal mechanisms that could mediate the beneficial effects of exercise on the prevention of gastric ulcer have not been fully described yet.…”

First Results of an Original Method Application of Experimental Stomach Ulcer Modeling

“…На сегодня моделированию язв желудка посвящено достаточно большое количество научных работ [1,2,3,4,5]. Наиболее часто применяется способ моделирования язвы желудка, предложенный S. Okabe et al [6,7].…”