Regucalcin plays a role in the cytoskeleton regulation of HepG2 cells

Chen, Jia; Xu, Beihui; Wu, Jugang; Liu, Xiangfan; Xu, Hong; Ni, Peihong

doi:10.1093/abbs/gmw122

Cited by 5 publications

(6 citation statements)

References 8 publications

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“…Of these various N-glycan modifications, at least core ␣1,3-fucose and galactosylated core ␣1,6fucose are potential anthelminthic targets as fungal lectins (specifically CCL2 and CGL2) recognising these elements are nematotoxic [25,26]. To date, our information regarding the enzymatic basis for modifications of nematode Nglycans is restricted to identification of the three fucosyltransferases modifying the core region (FUT-1, FUT-6 and FUT-8) and the ␣1,6-fucose-modifying galactosyltransferase (GALT-1) [19,[27][28][29] as well as conserved enzymes such as N-acetylglucosaminyltransferases I and II [30]. The genetic basis for further modifications remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Comparison of RP‐HPLC modes to analyse the N‐glycome of the free‐living nematode Pristionchus pacificus

2015

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Pristionchus pacificus is a free-living nematode increasingly used as an organism for comparison to the more familiar model Caenorhabditis elegans. In this study, we examined the N-glycans of this organism isolated after serial release with peptide:N-glycosidases F and A; after fluorescent labelling with 2-aminopyridine, chromatographic fractionation by three types of reversed-phase HPLC (with either classical C18, fused core C18 or alkylamide bonded phases) followed by mass spectrometric analyses revealed key features of its N-glycome. In addition to paucimannosidic and oligomannosidic glycans typical of invertebrates, N-glycans with two core fucose residues were detected. Furthermore, a range of glycans carrying up to three phosphorylcholine residues was observed whereas, unlike C. elegans, no tetrafucosylated N-glycans were detected. Structures with three fucose residues, unusual methylation of core α1,3-fucose or with galactosylated fucose motifs were found in low amounts; these features may correlate with a different ensemble or expression of glycosyltransferase genes as compared to C. elegans. From an analytical perspective, both the alkylamide RP-amide and fused core C18 columns, as compared to a classical C18 material, offer advantages in terms of resolution and of elution properties, as some minor pyridylamino-labelled glycans (e.g., those carrying phosphorylcholine) appear in earlier fractions and so potential losses of such structures due to insufficient gradient length can be avoided.

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Section: Introductionmentioning

confidence: 99%

Comparison of RP‐HPLC modes to analyse the N‐glycome of the free‐living nematode Pristionchus pacificus

2015

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“…Because most amino acids are not natively fluorescent, detection by LIF requires covalent modification of the analytes with a fluorescent tag. Currently, there are several commercially available labeling reagents such as naphthalene-2,3dicarboxaldehyde (NDA) [21,22], o-phthaldialdehyde [23,24], and 4-fluoro-7-nitro-2,1,3-benzoxadiazole [25]. NDA reacts quickly with primary amines in the presence of cyanide (or another nucleophile) to produce stable (cyano-benz(f)isoindole) derivatives that have fluorescence quantum yields ranging from 0.5 to 0.8 [21].…”

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confidence: 99%

Micellar electrokinetic chromatography method for measuring amino acid secretions from islets of Langerhans

Wang

Guillo

et al. 2015

Electrophoresis

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Islets of Langerhans are responsible for maintaining glucose homeostasis through regulated secretion of hormones and other factors. It is hypothesized that amino acids secreted from islets play a critical role in cell functionality and viability. For example, glutamate and gamma-aminobutyric acid (GABA) have been proposed to work as paracrine signaling molecules within islets to coordinate the release of hormone secretion; other amino acids, such as glutamine, leucine, alanine, and arginine have been shown to stimulate or potentiate glucose-stimulated insulin secretion. To characterize the potential roles that these small molecules may play in islet physiology, derivatization of amino acids in high salt buffers commonly used in islet experiments with naphthalene-2,3-dicarboxaldehyde and MEKC separation conditions were optimized. The optimized conditions used D-Norvaline as the internal standard and allowed quantification of 14 amino acids with LODs ranging from 0.2 – 7 nM. The RSD of the migration times were 0.04 – 0.54% and the RSD of the peak areas were 0.2 – 5.8% for the various amino acids. The effects of glucose and 2,4-dinitrophenol on amino acid secretions from islets were tested and a suppressive effect of glucose on GABA release was observed, likely acting through ATP inactivation of glutamate decarboxylase.

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“…Cells were pretreated with UV irradiation. Binding of p53 to the promoter and intron regions of FOXP3 was detected with a Pierce Agarose Chip Kit (Thermo) as described previously (28). Anti-p53 antibodies were used to immunoprecipitate the DNA segment.…”

Section: Chromatin Immunoprecipitation (Chip)mentioning

confidence: 99%

Ultraviolet irradiation promotes FOXP3 transcription via p53 in psoriasis

Zhang

Chen

et al. 2016

Experimental Dermatology

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The decrease of forkhead box P3-positive (FOXP3 + ) regulatory T cells (Tregs) causes an immune imbalance with effector T cells in psoriasis. Previous studies have demonstrated that in addition to its known effects on keratinocytes and effector T cells, ultraviolet (UV) irradiation alleviates psoriasis via the upregulation of FOXP3 + Tregs. However, the mechanism is unclear. Here, we found that FOXP3 + T cells were increased in psoriatic lesions after UVB irradiation (t' = 3.7006, P < 0.01), as determined by immunohistochemical staining. In addition, the levels of FOXP3 and p53, one of the downstream targets of UV irradiation, showed accordant changes after UV irradiation. Experiments that used a MAPK inhibitor, p53 mutant cell lines, p53 inhibitor and p53 shRNA showed a decrease in FOXP3 levels, suggesting that p53 is required for UV-induced FOXP3 transcription. Next, we demonstrated that there are two binding sites for p53 on FOXP3 by informatics tools, a dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay. One binding site (-1771 to -1583) is located at the promoter region and is adjacent to a previously reported p53-binding region in breast cancer cells. The other (+3845 to +4042) is located within the first intron and has not been previously reported. Our study demonstrated that FOXP3 is regulated, at least in part, by the binding of p53 to several binding sites in the promoter and intron regions following UV irradiation in psoriasis. It will be helpful to further clarify the regulatory mechanism of FOXP3 transcription and to provide new insights into the mechanisms that mediate the effects of UV irradiation in autoimmune skin disorders.

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Regucalcin plays a role in the cytoskeleton regulation of HepG2 cells

Cited by 5 publications

References 8 publications

Comparison of RP‐HPLC modes to analyse the N‐glycome of the free‐living nematode Pristionchus pacificus

Comparison of RP‐HPLC modes to analyse the N‐glycome of the free‐living nematode Pristionchus pacificus

Micellar electrokinetic chromatography method for measuring amino acid secretions from islets of Langerhans

Ultraviolet irradiation promotes FOXP3 transcription via p53 in psoriasis

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