Regucalcin enhances adipocyte differentiation and attenuates inflammation in 3T3‐L1 cells

Abstract

Dysregulation of adipocyte differentiation and dysfunction play key roles in the pathogenesis of obesity and associated disorders such as diabetes and metabolic syndrome, and as such, a better understanding of the molecular mechanism of adipogenesis may help to elucidate the pathological condition of obesity and its associated disorders. Regucalcin (RGN) plays multiple regulatory roles in intracellular Ca 2+ signaling pathways in mammalian cells. Here, we report that overexpression of RGN enhances lipid accumulation in 3T3‐L1 adipocyte cells after adipogenic stimulation, accompanied by upregulation of adipocyte differentiation marker proteins. In contrast, genetic disruption of RGN inhibited adipogenic stimulation‐induced differentiation of 3T3‐L1 cells. Furthermore, RGN overexpression in differentiated 3T3‐L1 adipocytes blocked inflammatory crosstalk between 3T3‐L1 adipocytes and RAW264.7 macrophages in a transwell coculture system. Knockdown of RGN expression in cocultured 3T3‐L1 adipocytes enhanced their susceptibility to RAW264.7 macrophage‐mediated inflammation. These results suggest that RGN is required for 3T3‐L1 adipocyte differentiation and that it exerts anti‐inflammatory activity against 3T3‐L1 adipocyte inflammation after coculture with RAW264.7 macrophages. Thus, RGN may be a novel regulator of adipocyte differentiation and act as a suppressor of inflammation in macrophage‐infiltrated adipocyte tissue.

Integration of transcriptomics and metabolomics reveals the molecular mechanisms underlying the effect of nafamostat mesylate on rhabdomyolysis-induced acute kidney injury

“…In agreement with the above studies, our results showed that NM offers kidney protection through reducing the pro-inflammatory cytokines IL-6 and TNF-α in RIAKI. Our data showed that NM also upregulated the anti-inflammatory gene Rgn , which is consistent with the report that Rgn may act as a suppressor of inflammation in macrophage-infiltrated adipocyte tissue ( Murata et al, 2020 ). Surprisingly, we found that NM induced the expression of the pro-inflammatory chemokine Cxcl13.…”

RGN as a prognostic biomarker with immune infiltration and ceRNA in lung squamous cell carcinoma

“…The research evidence of RGN in immune microenvironment is relatively insufficient. A study has demonstrated that RGN could exert anti-inflammatory effects on adipocyte cocultured with macrophages 48 . Our results also observed that the high RGN expression group often showed the low level of macrophage infiltrations, including macrophage M0 and M1.…”

Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

“…[17] Conventionally, impairment in adipocyte differentiation leads to obesity and insulin resistance. [18] 3T3-L1 preadipocytes differentiation is the well-established in vitro model for studying adipogenesis and an optimum platform to screen a variety of pro-adipogenic molecules that enhances differentiation at the cellular level. These cells were stimulated to differentiate in the presence of MDI (IBMX, DEXA, and Insulin cocktail) which is responsible for intracellular lipid accumulation.…”