Regrowth of embryogenic tissues of Pinus nigra following cryopreservation

Salaj, Terézia; Matušíková, Ildikó; Fráterová, Lenka; Piršelová, Beáta; Salaj, J.

doi:10.1007/s11240-010-9893-3

Cited by 46 publications

(27 citation statements)

References 29 publications

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“…1). The mass cell population could be attributed due to selection process; elimination of non embryonic cells from cultures or it may be due to increase synchrony of development of embryonic cells (Salaj et al, 2011). Hence, similar fi nding in present study show that cryopreserved PLBs of DBM retain high meristematic cells growing property after 18 months regenerated from vitrifi cation.…”

Section: Resultssupporting

confidence: 77%

Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses

2015

Mong. J. Biol. Sci.

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Cite this paper as:This study was conducted to detect the morphological, histological and molecular diff erences in the second generation of the PVS2 cryopreserved Dendrobium Bobby Messina [DBM] (18 months old culture) plantlets. Morphological analyses indicated that similarities and diff erences in cryopreserved DBM plantlets comparing to control stock culture based on selected morphological criteria. Morphological criteria, such as root length, number of shoot per explant and shoot length displayed diff erences, while the other three criteria, leaf diameter, leaf length and PLBs size were similar in cryopreserved compared to the control stock culture plant. Higher amount of homogenous cell population and denser cytoplasm were observed in cryopreserved PLBs compared to control stock culture PLBs based on histological analysis. This suggests the existance of somatic embryogenesis development mechanism taking place during the recovery and regeneration of the cryopreserved PLBs. However, RAPD analyses based on 10 primers indicated that cryopreserved DBM regenerated from vitrifi cation method generated a total of 20 to 39.9% polymorphic bands as compared to stock culture indicating potential somaclonal variation. Hence, an increase percentage of polymorphics bands in cryopreserved plantlets 18 months post cryopreservation as compared to previous report of 10% polymorphic bands in cryopreserved DBM 3 months post cryopreservation. Antony, J.

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Section: Resultssupporting

confidence: 77%

Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses

2015

Mong. J. Biol. Sci.

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“…In this study, in eight lines of ET (K1, K2, S3-S5, S7 -P. abies and AK2.2, AK2.9 -P. omorika) and the ET line recovered after cryostorage (AK2.6 -P. omorika), mutations occurred at more than one locus which suggests generally lower stability of their genomes. Other studies also indicated genotypedependent occurrence of somaclonal variation (Marum et al, 2009, Salaj et al, 2011. A recent study of Bradai et al (2016) on somaclonal variation in olive plants revealed clear relationship between genotype and susceptibility to the morphological and physiological alternations of regenerated plants.…”

Section: Discussionmentioning

confidence: 91%

“…Since both these processes may induce somaclonal variation (Sarmast, 2016), their influence needs to be evaluated and the obtained results might guide the improvement of propagation protocols. Also, our previous investigations were limited only to a single genotype which precludes any general concluding with regard to the intrinsic factors of somaclonal variation, i.e., genotypespecific propensity to mutate which is also a highly discussed issue in terms of somaclonal variability (Sahijram et al, 2003;Marum et al, 2009, Salaj et al, 2011, Bradai et al, 2016. In this work we wanted to asses if the prolonged time of in vitro proliferation and storage in LN affects the genetic integrity of genomes of two spruce species, P. abies and P. omorika.…”

Section: Introductionmentioning

confidence: 99%

Somaclonal Variation During Picea abies and P. omorika Somatic Embryogenesis and Cryopreservation

Hazubska-Przybył¹,

Dering²

2017

Acta Biologica Cracoviensia S. Botanica

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Embryogenic cultures of plants are exposed to various stress factors both in vitro and during cryostorage. In order to safely include the plant material obtained by somatic embryogenesis in combination with cryopreservation for breeding programs, it is necessary to monitor its genetic stability. The aim of the present study was the assessment of somaclonal variation in plant material obtained from embryogenic cultures of Picea abies (L.) Karst. and P. omorika (Pančić) Purk. maintained in vitro or stored in liquid nitrogen by the pregrowth-dehydration method. The analysis of genetic conformity with using microsatellite markers was performed on cotyledonary somatic embryos (CSE), germinating somatic embryos (GSE) and somatic seedlings (SS), obtained from tissues maintained in vitro or from recovered embryogenic tissues (ETc) and CSE obtained after cryopreservation. The analysis revealed changes in the DNA of somatic embryogenesis-derived plant material of both Picea spp. They were found in plant material from 8 out of 10 tested embryogenic lines of P. abies and in 10 out of 19 embryogenic lines of P. omorika after in vitro culture. Changes were also detected in plant material obtained after cryopreservation. Somaclonal variation was observed in ETc and CSE of P. omorika and at ETv stage of P. abies. However, most of the changes were induced at the stage of somatic embryogenesis initiation. These results confirm the need for monitoring the genetic stability of plants obtained by somatic embryogenesis and after cryopreservation for both spruce species.

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“…The RAPD technology has previously been used successfully to detect occurrence of genetic alterations (Finkle et al, 1985;Harding, 1997;Aronen et al, 1999;Ahuja et al, 2002;Urbanova´ et al, 2005;Castillo et al, 2010), but this approach possesses limits with reproducibility, and it is currently being replaced by techniques such as Amplified Fragment Length Polymorphisms (AFLPs) and/or Simple Sequence Repeats (SSRs, microsatellites). These techniques are now being used to consider more carefully the issue of genetic fidelity after cryo-procedures, especially in the breeding of long-living conifers (Salaj et al, 2010), where genetic changes might be substantially expressed only later on, in mature trees.…”

Section: Variation In Cryopreserved Derived Plantmentioning

confidence: 99%

Fundamental Cryobiology and Basic Physical, Thermodynamical and Chemical Aspects of Plant Tissue Cryopreservation

Zeliang¹,

Pattanayak²

2012

Plant Breeding

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Regrowth of embryogenic tissues of Pinus nigra following cryopreservation

Cited by 46 publications

References 29 publications

Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses

Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses

Somaclonal Variation During Picea abies and P. omorika Somatic Embryogenesis and Cryopreservation

Fundamental Cryobiology and Basic Physical, Thermodynamical and Chemical Aspects of Plant Tissue Cryopreservation

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