Regression of endometrial explants in a rat model of endometriosis treated with the immune modulators loxoribine and levamisole

Keenan, Jeffrey; Williams-Boyce, Patsy K.; Massey, Pamela J.; Chen, Thomas T.; Caudle, Michael R.; Bukovský, Antonín

doi:10.1016/s0015-0282(99)00157-0

Cited by 151 publications

(75 citation statements)

References 29 publications

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“…Conceptually, the lesions in those animals could not be regarded as endometriosis, as the disease is characterized by the presence of glandular and/or stromal tissue outside the uterine cavity. However, if the animals with a score of zero were included, the mean of scores would be 1.3 and 1.4 in Groups 1 and 2, respectively, which is comparable to the mean of the control group scores in Keenan's study 30 . Although the expression "experimental endometriosis" is used in the literature, strictly speaking, the present study involved fragments of normal endometrium on the abdominal wall of healthy rats.…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 54%

“…According to the anatomopathological classification proposed by Keenan 30 , eight animals had a score of zero. Conceptually, the lesions in those animals could not be regarded as endometriosis, as the disease is characterized by the presence of glandular and/or stromal tissue outside the uterine cavity.…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

“…The persistence of epithelial cells in the implants was evaluated according to the classification proposed by Keenan 30 , as follows: well-preserved epithelial layer = score 3, moderately preserved epithelium with leukocyte infiltrate = score 2, poorly-preserved epithelium (occasional epithelial cells only) = score 1, no epithelium = score zero.…”

Section: Methods Methods Methods Methodsmentioning

confidence: 99%

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Desenvolvimento de modelo experimental de endometriose em ratas

Amaral

Lago

Kondo

et al. 2009

Rev. Col. Bras. Cir.

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OBJETIVO: Desenvolver um modelo de endometriose experimental em ratas. MÉTODOS: Foram utilizadas 30 ratas adultas da linhagem Wistar. A técnica cirúrgica consistiu em laparotomia mediana com identificação do útero bicorno e ressecção de um segmento de 2 cm do corno uterino direito. Um retalho de 0,25 cm² foi retirado dessa estrutura e suturado na parede abdominal com a face endometrial voltada para a cavidade peritoneal. As ratas foram divididas aleatoriamente em dois grupos de acordo com o tempo para a reoperação: grupo 1 (n=15), reoperado em 30 dias, e grupo 2 (n=15), em 60 dias. No momento da segunda laparotomia os implantes foram avaliados macroscopicamente, ressecados e encaminhados para análise microscópica com coloração hematoxilina-eosina e imunohistoquímica (HEMA, AE1 e AE2). RESULTADOS: Os implantes se desenvolveram em 83,3 % do Grupo 1 e 71,4% no Grupo 2. Não houve diferença estatisticamente significativa entre o peso dos animals dos dois grupos. Também não houve diferença estatisticamente significativa no tamanho da área das lesões induzidas: no Grupo 1 a média foi 0,37 cm² e no Grupo 2, de 0,25 cm². Segundo a classificação histológica semi-quantitativa de Keenan (de acordo com a preservação da camada epitelial de endométrio), o Grupo 1 teve média de 1,9 e o Grupo 2, de 2,4. CONCLUSÃO: A técnica utilizada para o desenvolvimento de endometriose em ratas foi satisfatória.

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Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 54%

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

Section: Methods Methods Methods Methodsmentioning

confidence: 99%

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Desenvolvimento de modelo experimental de endometriose em ratas

Amaral

Lago

Kondo

et al. 2009

Rev. Col. Bras. Cir.

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“…The development of experimental endometriosis in female rats through auto-transplantation techniques prompted research on additional medical treatments for endometriosis. These treatments were analyzed for their efficacy via macroscopic and histological parameters, among others 9,10 . Because of the known properties of Copaiferalangsdorffii and its relationship to mechanisms involved in the etiopathogeny of endometriosis, the present study aimed to analyze its effects on experimental endometriosis implants in rats.…”

Section: Introductionmentioning

confidence: 99%

Changes in the volume and histology of endometriosis foci in rats treated with copaiba oil (Copaiferalangsdorffii)

Neto

Lindoso²,

Coelho³

et al. 2011

Acta Cir. Bras.

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PURPOSE: The aim of this study was to analyze the changes that occur in rats with experimental endometriosis after treatment with copaiba oil. METHODS: Experimental endometriosis was induced in rats. The experimental group received copaiba oil (Copaiferalangsdorffii) orally (0.63 mg/day), and the control group received a 0.9% sodium chloride solution orally (1 ml/100 g of body weight/day). Both groups were treated with gavage for 14 days. After this period, the animals were euthanized, and the implant volume was calculated. The autologous transplants were removed, dyed with hematoxylin-eosin, and analyzed by light microscopy. RESULTS: The average final volumes were significantly different between the groups (p=0.007). There was a significant increase (p=0.012) between the initial and final volumes in the control group, whereas treatment with Copaiferalangsdorffii caused a marked reduction in endometrial growth over time (p=0.016). Histologically, 6/11 (55.00%) rats in the experimental group had a well-preserved epithelial layer, and 3 (45.00%) had mildly preserved epithelium. The control group had seven cases (58.30%) of well-preserved epithelial cells and five cases (41.70%) of mildly preserved epithelial cells (p>0.05). CONCLUSION: Copaiba oil (Copaiferalangsdorffii) appears to be a promising alternative treatment for endometriosis.

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“…5 The tre at ment of en do metri o sis was in ves ti ga ted in ear li er stu di es with a rat mo del using GnRHa and go na dot ro pin re le a sing hor mo ne an ta go nists and va ri o us agents such as pen to xiphy lli ne, doxyc ycli ne, and le va mi zol. [5][6][7][8][9] GnRH ana lo gu es are be ing used ef fec ti vely in the tre at ment of en do met ri o sis and the re is a con sensus in the cur rent li te ra tu re that the se agents are ef fec ti ve. 5,9 The use of GnRHa with monthly injec ti ons or in jec ti ons with 3-month in ter vals in hibits ova ri an es tro gen synthe sis and forms a hypo es tro ge nic en vi ron ment, thus di mi nis hing the en do met ri o sis fo ci.…”

mentioning

confidence: 99%

Therapeutic Efficiency of Tibolone in a Rat Endometriosis Model

Şimşek¹,

Ceyhan²,

Üstün³

et al. 2011

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : This study ai med to eva lu a te the the ra pe u tic ef fi ci ency of ti bo lo ne in an ex pe ri men tal rat mo del of in tra-ab do mi nal en do met ri o sis. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : In this ex pe rimen tal study, in tra-ab do mi nal en do met ri o sis was in du ced in 30 fe ma le rats sur gi cally. Fo ur we eks af ter this pro ce du re, la pa ro tomy was per for med again. The di men si ons of the en do met ri o sis fo ci were re cor ded and right af ter, the ope ra ti on was comp le ted. Rats we re ran domly di vi ded in to thre e gro ups and sub se qu ently, the tre at ment was star ted. In the first gro up (n= 8), a sing le do se of 1 cc 0.9% NaCl was in jec ted sub cu ta ne o usly. In the se cond gro up (n= 8), in tra mus cu lar in jec ti on of 1 mg le up ro lid ace ta te was ad mi nis te red. In the third gro up (n= 8), 1 mg/kg/day of ti bo lo ne was gi ven by ga va ge. At the end of 4-we eks of drug ad mi nis tra ti on, la pa ro tomy was per for med to all rats. The di men si ons of the en do met ri o sis fo ci we re re cor ded. Af ter wards, all the rats we re sac ri fi ced. The dif fe ren ces in the are as of en do met ri o tic imp lants and ad he si on sco res we re com pa red bet we en the gro ups. R Re e s su ul lt ts s: : The di men si ons of the en do met ri o sis fo ci in the gro ups tre a ted with le up ro lid (p< 0.05) and ti bo lo ne (p< 0.05) we re sig ni fi cantly di mi nis hed compared to that of the con trol gro up. No sta tis ti cally sig ni fi cant dif fe ren ces we re fo und bet we en the tre at ment gro ups. C Co on nc c l lu u s si i o on n: : In a rat en do met ri o sis mo del, ti bo lo ne has a si mi lar ef fi ci ency as that of le up ro lid ace ta te, an agent used for con ven ti o nal me di cal tre at ment of en do met ri o sis. With its an dro ge nic and pro ges ta ge nic charac te ris tics, ti bo lo ne de ser ves at ten ti on as an al ter na ti ve agent in the me di cal tre at ment of en domet ri o sis in hu man. K Ke ey y W Wo or rd ds s: : Rats; endometriosis; gonadotropin-releasing hormone; tibolone Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da ka rın içi en do met ri o zis li de ney sel sı çan mo de lin de ti bo lon un te ra pö tik et kin li ği nin de ğer len di ril me si amaç lan dı. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Bu de ney sel ça lış ma da, 30 di şi sı çan -da ka rın içi cer ra hi en do met ri o zis mey da na ge ti ril di. Bu iş lem den dört haf ta son ra tekrar la pa ro tomi ya pıl dı. En do met ri o zis odak la rı nın bo yut la rı kay de dil di he men ar dın dan ope ras yon ta mam lan dı. Sı çan lar rast ge le üç gru ba ay rıl dı ve da ha son ra te da vi baş lan dı. Bi rin ci gru ba (n= 8), NaCl 1 cc %0.9 tek doz ola rak cilt al tı na en jek te edil di. İkin ci gru ba (n= 8), le up ro lid ase tat 1 mg in tra mus kü ler ola rak uy gu lan dı. Üçün cü gru ba (n= 8), ti bo lon 1mg/kg/gün ola rak ga vaj ile ve ril di. Dört haf ta ilaç alı mı nın son ra s...

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Regression of endometrial explants in a rat model of endometriosis treated with the immune modulators loxoribine and levamisole

Cited by 151 publications

References 29 publications

Desenvolvimento de modelo experimental de endometriose em ratas

Desenvolvimento de modelo experimental de endometriose em ratas

Changes in the volume and histology of endometriosis foci in rats treated with copaiba oil (Copaiferalangsdorffii)

Therapeutic Efficiency of Tibolone in a Rat Endometriosis Model

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