Regression data processing methods for parallel zero- and first-order processes applied to lactate dehydrogenase subunit determinations

Simultaneous determination of mixtures by kinetic analysis of general-order reactions

“…Examples of these methods include multicomponents with first-order reaction,2,5,6,9,23 first and zero order,7,22 and parallel first and zero order. 8 A related subject that is equivalent to the first-order case is the extensively studied subject of analysis of multicomponent fluorescence decay kinetics (e.g., refs 11-13).…”

Evaluation of multipoint kinetic methods for immunoassays: kinetic quantitation and immunoglobulin G

“…Typically, data acquired on any given day were stored on the disk and later transferred via RS-232C serial lines to a more powerful computer (MCS-510 workstation, Massachusetts Computer Corp., Westford, MA) for further processing, graphics output, and long-term storage. We have translated the data-processing programs reported previously (8,9) from FORTRAN IV into C language and have modified them to provide autoranging and automatic determination of initial estimates used in fitting data to a combined first-order/zero-order model described in detail below. In addition, a small data-base program has been written to store kinetic data from several different laboratory instruments in a unified format.…”

“…Its measurement in natural waters has attracted interest in part because of its recently recognized role in converting S02 to H2S04 in aqueous aerosols (4,5). A variety of analytical approaches are available for H202, including chemiluminescence methods (6)(7)(8)(9)(10)(11), spectrophotometric methods (12,13), and fluorometric methods (1-3,14, 15). A major concern in the development of these methods has been achievement of sub-micromolar detection limits without interference from the numerous other oxidizing substances found in natural waters.…”

“…In recent years we have developed and evaluated a variety of multipoint procedures for kinetic methods (8)(9)(10). An objective of this study was to evaluate the potential utility of these multipoint-kinetic methods for immunochemical reactions.…”

Multipoint kinetic method for the immunochemical quantitation of isoenzymes developed and evaluated with creatine kinase inhibition as a model system

“…To use the equation, curve-fitting methods described previously (3, [6][7][8] are used to obtain the values of A¡, ku and initial velocities for the first-order (Vy) and zero-order (Vi 0) components that give the best fits of data for At Vs. t to the zero-order/first-order model represented by eq 6. The computed values of Vy and Vi 0 are proportional to the activities of the inhibited and uninhibited components of the enzyme.…”