Regression coefficient of semen characteristics of buffalo bulls on seminal plasma proteins

Abstract

An experiment was carried out to explore the regression coefficients (RC) of semen characteristics on seminal proteins of buffalo bulls. SDS-PAGE resolved seminal proteins. A life sperm (LS) percentage exhibited a negative and significant RC on PF1 (111.5 ± 18.0 kDa), PF4 (36.13 ± 3.33 kDa), and PF5 (28. 88 ± 3.26 kDa) proteins, namely -0.13, -0.63 and -0.69 % / kDa respectively. A sperm acrosome integrity (SAI) percentage exhibited a pronounced negative RC on PF1 and PF2 (87.63 ± 16.5 kDa), -0.13 and -0.14 % / kDa, respectively. A negative RC was noticed in SAI percentage on PF4 (-0.61 % / kDa) and PF5 (-0.60 % / kDa) proteins. A pronounced positive RC was shown for total sperm abnormalities (TSA) on PF1 proteins (0.02 % / kDa). In conclusion, some semen attributes had a negative RC on some seminal proteins. In conclusion, some semen attributes had a negative RC on seminal proteins. These provide us with a good tool for early predicting the buffalo bull’s fertility to reduce the economic losses of male infertility and enhance the developmental sustainability of buffalo production in Iraq.