Registration of ‘USDA Rattler’ pinto bean

Abstract

The pinto bean (Phaseolus vulgaris L.), 'USDA Rattler' (Reg. no. CV-350, PI 693789), was released by the USDA-ARS in 2021 as a high-yielding, upright, fullseason cultivar with abiotic stress tolerance. It was bred for broad adaptation to the different bean production regions across the United States by selection for high yield performance under nonstress and multiple stress trials in Washington state. The multiple stress trials included a "purgatory" plot with low fertility, intermittent drought, and soil compaction, and a separate trial which simulated terminal drought.Conversely, the nonstress trials were well watered and fertilized to optimize yield potential. Yield stability is evidenced by the mean seed yield for the breeding line PT11-13, from which USDA Rattler was selected, numerically exceeding the trial means for 33 of 38 location-years. Broad adaptation for USDA Rattler is further evidenced by an average seed yield of 4547 kg ha −1 across 10 location-years in the Cooperative Dry Bean Nursery. Genetic markers as well as pathogen testing indicate USDA Rattler has I and bc-3 gene combination for durable resistance to all known strains of Bean common mosaic virus and Bean common mosaic necrosis virus. Greenhouse testing with four races of the rust pathogen indicated USDA Rattler is fixed for the Ur-3 resistance gene but is heterogenous for presence of the Ur-11 resistance gene. The seed size, appearance, and canning quality characteristics of USDA Rattler meets the industry standards for packaging and processing.

Registration of ‘USDA Diamondback’ slow‐darkening pinto bean

“…Precipitation averages <60 mm during the growing season, so intermittent drought is easily attained by withholding overhead irrigation applications to match 30%–50% of evapotranspiration. Through the years the purgatory plot has been useful for identifying lines and developing cultivars with abiotic stress tolerance (Miklas et al., 2023; Trapp et al., 2015). PT13‐3 was selected for advanced testing as an F 2:5 line in 2014 in the non‐stress (Othello) and purgatory plot (Prosser) trials.…”

“…Inoculation of seedlings in the Prosser greenhouse with US‐6 strain of BCMV and NL‐3 strain of BCMNV, following protocols from Miklas et al. (2023), were used to screen lines for disease reactions indicating the presence of specific resistance genes (Kelly, 1997). The dominant I gene and recessive bc‐u d , bc‐1 , bc‐2 , and bc‐3 genes conditioning resistance to BCMV and BCMNV were specifically detected by SNP or InDel (Insertion or Deletion) markers using Tm‐shift assays (Soler‐Garzón et al., 2021a, 2021b).…”

“…Inoculation of seedlings in the Prosser greenhouse with US-6 strain of BCMV and NL-3 strain of BCMNV, following protocols from Miklas et al (2023), were used to screen (Urrea, 2020(Urrea, , 2021(Urrea, , 2022. lines for disease reactions indicating the presence of specific resistance genes (Kelly, 1997).…”

“…The USDA‐ARS program uses contrasting non‐stress and multiple‐stress trial environments to develop breeding lines that perform well across variable production environments (Miklas et al., 2022). ‘USDA Rattler’, a recent pinto bean release (Miklas et al., 2023) resulting from this program of selection under contrasting nonstress and stress environments, has exhibited high seed yields above the trial means in Nebraska, North Dakota, Washington, and Wyoming.…”