Registration of Two Cranberry Bean Germplasm Lines Resistant to Bean Common Mosaic and Necrosis Potyviruses

Miklas, Phillip N.; Kelly, James D.

doi:10.2135/cropsci2002.0673

Cited by 8 publications

(8 citation statements)

References 3 publications

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“…Sutter Pink (HG 0), Bountiful (HG 1), Redlands Greenleaf C (HG 2), Redlands Greenleaf B (HG 3), UI-123 (HG 3), UI-34 (HG 4), UI-114-8 (HG 5), UI-31 (HG 6), IVT-7214 (HG 7), Black Turtle 1 (HG 8), Top Crop (HG 9), Jubila (HG 9), Red Kloud (HG 10), US92-1006 (HG 11), and TARS-VR-7s (HG 12). An additional set of bean germplasm lines developed by Miklas et al (16)(17)(18)(19) with resistance to BCMV and BCMNV also were evaluated for resistance to infection with NL-3 K or NL-3 D. Lines USLK-1, USLK-2, USLK-3, USDK-4, USDK-5, USWK-6, USCR-7, and USCR-9 each contained the resistance genes I + bc3, whereas USCR-8 contained only the bc3 gene (20). Infected source tissue was macerated in cold 50 mM potassium phosphate, pH 7.4, containing 10 mM sodium sulfite.…”

Section: Methodsmentioning

confidence: 99%

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

Larsen¹,

Miklas²,

Druffel³

et al. 2005

Phytopathology®

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A strain of Bean common mosaic necrosis virus (BCMNV) from Idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the NL-3 D strain (of Drifjhout) by reaction of differential bean cultivars. However, this BCMNV strain (designated NL-3 K) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. The nucleotide sequence encoding the predicted polyprotein of NL-3 K was 9,893 nucleotides (nt) in length, yielding a peptide with a molecular size of 362.1 kDa compared with a 9,626-nt, 350.9-kDa polyprotein for NL-3 D. Sequence analysis of the putative P1 protein suggests that the NL-3 K strain is a recombinant between NL-3 D and the Russian strain (RU1) of Bean common mosaic virus. The P1 protein of NL-3 K consisted of 415 amino acids compared with 317 for NL-3 D. The first 114 predicted amino acids of the NL-3 K P1 region were 98% identical with RU1. The remaining 301 amino acids of the protein shared only 34% identity with RU1 but were 98% identical with NL-3 D. Primers were designed that flanked the recombination point in the P1 coding sequence of NL-3 K. An amplicon of the expected size was produced by reverse-transcriptase polymerase chain reaction of total nucleic acid extracts of bean plants inoculated with NL-3 K, but not from those with NL-3 D or RU1. The increased symptom severity on selected common bean lines induced by NL-3 K suggests that the P1 gene may play a significant role in pathogenicity and virulence.

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Section: Methodsmentioning

confidence: 99%

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

Larsen¹,

Miklas²,

Druffel³

et al. 2005

Phytopathology®

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“…A marker tightly linked to the I gene (Haley et al, 1994b;Melotto et al, 1996) has been demonstrated in many laboratories to be effective across a wide range of germplasm from both gene pools. Breeders (Kelly et al, 1994;Miklas et al, 2002a;Miklas & Kelly, 2002) have used markers linked to the I gene to develop enhanced germplasm with the I + bc-3 gene combination. A linkage distance of ∼5 cM between the I gene and linked SW13 marker may result in recombinants that possess the marker but lack the gene (Vandemark & Miklas, 2005), so pathogen testing a final testcross is recommended to confirm presence of the I gene.…”

Section: Bean Common Mosaic Virus (Bcmv) and Bean Common Mosaic Necromentioning

confidence: 99%

Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding

Miklas

Kelly

Beebe³

et al. 2006

Euphytica

478

462

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Breeding for resistance to biotic and abiotic stresses of global importance in common bean is reviewed with emphasis on development and application of marker-assisted selection (MAS). The implementation and adoption of MAS in breeding for disease resistance is advanced compared to the implementation of MAS for insect and abiotic stress resistance. Highlighted examples of breeding in common bean using molecular markers reveal the role and success of MAS in gene pyramiding, rapidly deploying resistance genes via marker-assisted backcrossing, enabling simpler detection and selection of resistance genes in absence of the pathogen, and contributing to simplified breeding of complex traits by detection and indirect selection of quantitative trait loci (QTL) with major effects. The current status of MAS in breeding for resistance to angular leaf spot, anthracnose, Bean common mosaic and Bean common mosaic necrosis viruses, Beet curly top virus, Bean golden yellow mosaic virus, common bacterial blight, halo bacterial blight, rust, root rots, and white mold is reviewed in detail. Cumulative mapping of disease resistance traits has revealed new resistance gene clusters while adding to others, and reinforces the co-location of QTL conditioning resistance with specific resistance genes and defense-related genes. Breeding for resistance to insect pests is updated for bean pod weevil (Apion), bruchid seed weevils, leafhopper, thrips, bean fly, and whitefly, including the use of arcelin proteins as selectable markers for resistance to bruchid seed weevils. Breeding for resistance to abiotic stresses concentrates on drought, low soil phosphorus, and improved symbiotic nitrogen fixation. The combination of root growth and morphology traits, phosphorus uptake mechanisms, root acid exudation, and other traits in alleviating phosphorus deficiency, and identification of numerous QTL of relatively minor effect associated with each trait, reveals the complexity to be addressed in breeding for abiotic stress resistance in common bean.

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“…Through an exceptional nationwide informal collaborative effort, pathologists and breeders have pyramided I and bc-3 resistance alleles, and combined them with rust resistance into great northern BelMiNeb-RMR-6 to 13 and pinto BelDakMi-RMR-14 to 23 beans (Pastor-Corrales, 2003;Pastor-Corrales et al, 2001). Miklas and Kelly (2002) developed cranberry and Miklas et al (2002b) developed light and dark red and white kidney bean germplasm lines resistant to BCMV and BCMNV. In addition to molecular markers, a clearer understanding of the evolutionary origin (Andean vs. Middle American) of the I resistance allele and its undesirable linkage with the B allele for seed coat color (Kyle and Dickson, 1988;Temple and Morales, 1986) should facilitate breeding.…”

Section: Bcmv and Bcmnv Resistancementioning

confidence: 99%

“…After 40 years it is still a popular cultivar. Good canning quality and resistance to anthracnose, BCMV, BCTV, and rust, either singly or in combinations, have also been bred into other dark red kidney, light red kidney, white kidney, and cranberry beans for North America (Kelly et al, 1998b, 1999b, 1999cMiklas and Kelly, 2002;Miklas et al, 2002b). , Beaver and Steadman (1999), and Beaver et al (2003) described breeding for race Nueva Granada cultivars for the tropics and subtropics.…”

Section: Race Nueva Granada Cultivarsmentioning

confidence: 99%

Common Bean ( Phaseolus vulgaris L.)

Singh¹

2005

Genetic Resources, Chromosome Engineering, and Crop Improvement

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Registration of Two Cranberry Bean Germplasm Lines Resistant to Bean Common Mosaic and Necrosis Potyviruses

Cited by 8 publications

References 3 publications

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding

Common Bean ( Phaseolus vulgaris L.)

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