Registration of LONREN-1 and LONREN-2 Germplasm Lines of Upland Cotton Resistant to Reniform Nematode

Abstract

Cotton (Gossypium hirsutum L.) germplasm lines LONREN-1 (Reg. No. GP-977, PI 669509) and LONREN-2 (Reg. No. GP-978, PI 669510) were developed and released by the USDA-ARS, Texas Agricultural Experiment Station and Cotton Incorporated in 2007 to provide breeders with desirable germplasm resistant to the reniform nematode (Rotylenchulus reniformis Linford and Oliveira). The resistance was transferred from wild G. longicalyx via a triple-species hybrid. Crosses, backcrosses, and single plant selections were used to develop the F 2 progeny used for seed production. Resistance was followed with the codominant simple sequence repeat BNL 3279_114 marker, located 1.4 cM proximal and the phenotypic marker 'greenfuzz' (LTCOL_F) located 4.5 cM distal to the Ren lon resistance gene on chromosome 11. A single nucleotide polymorphism marker also was developed for rapid screening of large numbers of seed. The introgressed chromosome segment is smaller in LONREN-2 than in LONREN-1. Both lines reduced reniform populations by 95% in growth chamber bioassays and by 50 to 90% in ields. In the absence of nematodes in the ield, growth rate, yield, and iber quality of the lines were similar to that of the 'Fibermax 958' parent. In the presence of reniform nematodes in the ield, the lines often showed stunting and yield losses, probably due to enhanced severity of fungal seedling diseases, especially Thielaviopsis root rot. The seedling diseases in LONRENs were diminished by control measures such as fungicides, nematicides, and crop rotation with corn or sorghum, and were negligible in a second year of planting in the same ield.

SNP‐Based MAS in Cotton under Depressed‐Recombination for Renlon–Flanking Recombinants: Results and Inferences on Wide‐Cross Breeding Strategies

“…Development of the tri‐species hybrids was summarized by Robinson et al (2007). Here, we used two released LONREN lines (Bell et al, 2014), as well as a number of other backcross‐inbred lines that contain Ren lon and differ in pedigree and/or alien segment constitution (Table 1).…”

“…The worsening problem of reniform nematode in cotton production led to a research effort to incorporate genetic resistance to the reniform nematode in Upland cotton genotypes (Nichols et al, 2014). All Upland cotton cultivars lack significant resistance to reniform nematode (Koenning et al, 2004; Robinson et al, 1999; Usery et al, 2005; Weaver et al, 2007), therefore scientists at several laboratories pursued introgression of reniform nematode resistance from other Gossypium species (Avila et al, 2006; Bell et al, 2014, 2015; Robinson et al, 2007; Romano et al, 2009).…”

“…Segregation of the reniform nematode resistance suggested monogenic control, and linkage with G. hirsutum chromosome‐11 (c11) molecular and phenotypic markers indicated a single dominant gene or haplotype, Ren lon (Dighe et al, 2009). Two reniform resistant germplasm lines LONREN‐1 and LONREN‐2 were subsequently released (Bell et al, 2014). Evaluations of the LONREN lines and derived materials in diverse field environments revealed that plants with the alien Ren lon ‐bearing segment exhibited early season stunting that was pronounced in certain cases when grown in heavily nematode‐infested fields, even though they suppressed nematodes.…”

Genetic characterization of Gossypium arboreum accession PI 529740 for reniform nematode resistance

“…Resistance has been successfully introgressed into G. hirsutum from G. longicalyx (Hutch. & Lee), using a hexaploid bridging line to derive tetraploid trispecies hybrids , resulting in the release of two germplasm lines (Bell et al, 2014).…”

Genetic characterization of reniform nematode resistance for Gossypium arboreum accession PI 417895

“…Gossypium longicalyx was the only cotton species to show immunity and was classified as a non-host, because R. reniformis females penetrating the roots system did not mature or produce eggs Yik & Birchfield, 1984). The G. longicalyx source of resistance has been successfully introgressed into G. hirsutum using a hexaploid bridging line to derive tetraploid trispecies hybrids , and two germplasm lines LONREN-1 (PI 669509) and LONREN-2 (PI 669510) have been released (Bell et al, 2014). These lines unfortunately showed severe root necrosis and progressive root mass loss under high levels of R. reniformis inoculum (Sikkens, Weaver, Lawrence, Moore, & van Santen, 2011).…”