Registration of four upland cotton germplasm lines with elevated levels of seed oil oleic acid

Dowd, Michael K.; McCarty, Jack C.; Shockey, Jay; Jenkins, Johnie N.

doi:10.1002/plr2.20017

Cited by 8 publications

(10 citation statements)

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“…todes, three lines exhibited some level of elevated oleic acid (Dowd et al, 2020). The M Rk-Rn 1 (PI 678938) and M Rk-Rn 5 (PI 678942) lines each exhibited elevated oleate levels in two of the five seeds tested with levels that varied between 31% and 44%.…”

Section: Core Ideasmentioning

confidence: 96%

“…One high-oleate accession of interest, GB713 (PI 608139), had also been reported to have high levels of resistance to reniform nematodes (Robinson et al, 2004) and had been used to breed this trait into the G. hirsutum cultivar Sure-Grow 747 (SG747, PVP 9800118) to produce two sets of nematode-resistant G. hirsutum plant lines (McCarty et al, 2013(McCarty et al, , 2017. Evaluation of individual seeds from these lines revealed elevated oleate levels in several seeds (Dowd et al, 2020). One of the three released M713Ren lines (M713Ren1, PI 665928) had elevated oleic acid levels (30%-35%) in two of the five seeds tested.…”

Section: Crop Sciencementioning

confidence: 99%

“…Breeding between this plant line, M‐240 RNR (PI 592511), and SG747 added multiple gene alleles for root‐knot nematode resistance (McCarty et al., 2017). Of the six M Rk‐Rn lines produced with resistance to both nematodes, three lines exhibited some level of elevated oleic acid (Dowd et al., 2020). The M Rk‐Rn 1 (PI 678938) and M Rk‐Rn 5 (PI 678942) lines each exhibited elevated oleate levels in two of the five seeds tested with levels that varied between 31% and 44%.…”

Section: Introductionmentioning

confidence: 99%

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Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm

Dowd,

Shockey,

McCarty

et al. 2023

Crop Science

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A high seed oil oleate trait identified in wild Gossypium barbadense L. cotton accession GB713 (PI 608139) was bred into Upland cotton (G. hirsutum L.) cultivar ‘Sure‐Grow 747’ (SG747, PVP 9800118). Starting from the F2 generation of an initial cross between GB713 and SG747, plants were selected by measuring the seed oil fatty acid distributions by gas chromatography. Selected F3 plants were crossed to M Rk‐Rn 6, a line previously created from GB713 and SG747 for improved nematode resistance. High‐oleate plants were selected in the F2 population. Selected plants were then crossed to HOa1 (a ∼35% oleate line derived from M Rk‐Rn 1, a sibling line to M Rk‐Rn 6), and high‐oleate plants were again selected in the F2 population. F3 plants were re‐selected for the high‐oleate trait, and four selected F4 lines were evaluated in 2022 field plots. The four lines had oleate levels between 52 and 57%, approximately triple that found in normal cottonseed oil (15‒19%) and greater than in either the high‐oleate wild GB713 parent (∼40%) or in the previously released HOa cotton plant lines (33‒35%). The increased oleate levels occurred with an approximately equal reduction in linoleic acid. Other seed and fiber properties were largely like those of SG747, although some residual GB713 character existed within the lines. The resulting oil composition should be ideal for use in deep fat frying, with the high level of oleic acid improving the oil's oxidative stability but with sufficient linoleic acid to maintain frying flavor.This article is protected by copyright. All rights reserved

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Section: Core Ideasmentioning

confidence: 96%

Section: Crop Sciencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm

Dowd,

Shockey,

McCarty

et al. 2023

Crop Science

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“…In addition, RNAi-directed down-regulation of PEPC2 up-regulated most lipid synthesis-related genes, resulting in 7.3% increase in cottonseed oil content ( Zhao et al, 2018a ). Shockey et al (2017) and Sturtevant et al (2017) identified a natural mutant allele of FAD2-1D in G. barbadense with high oleic acid in cottonseed oil, and its incorporation into G. hirsutum doubled oleic acid content ( Dowd et al, 2020 ). Most recently, Chen et al (2021) confirmed that knockout mutants of the GhFAD2 genes in Upland cotton by CRISPR/Cas9 editing increased the oleic acid level to 77.7% with a concomitant decrease in linoleic acid (from 58.6 to 6.9%) and palmitic acid (from 23.95 to 13.18%).…”

Section: Genetic Engineering Of Cotton For Improving Cottonseed Oil F...mentioning

confidence: 99%

Genetics, Breeding and Genetic Engineering to Improve Cottonseed Oil and Protein: A Review

Pei

Wedegaertner

et al. 2022

Front. Plant Sci.

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Upland cotton (Gossypium hirsutum) is the world’s leading fiber crop and one of the most important oilseed crops. Genetic improvement of cotton has primarily focused on fiber yield and quality. However, there is an increased interest and demand for enhanced cottonseed traits, including protein, oil, fatty acids, and amino acids for broad food, feed and biofuel applications. As a byproduct of cotton production, cottonseed is an important source of edible oil in many countries and could also be a vital source of protein for human consumption. The focus of cotton breeding on high yield and better fiber quality has substantially reduced the natural genetic variation available for effective cottonseed quality improvement within Upland cotton. However, genetic variation in cottonseed oil and protein content exists within the genus of Gossypium and cultivated cotton. A plethora of genes and quantitative trait loci (QTLs) (associated with cottonseed oil, fatty acids, protein and amino acids) have been identified, providing important information for genetic improvement of cottonseed quality. Genetic engineering in cotton through RNA interference and insertions of additional genes of other genetic sources, in addition to the more recent development of genome editing technology has achieved considerable progress in altering the relative levels of protein, oil, fatty acid profile, and amino acids composition in cottonseed for enhanced nutritional value and expanded industrial applications. The objective of this review is to summarize and discuss the cottonseed oil biosynthetic pathway and major genes involved, genetic basis of cottonseed oil and protein content, genetic engineering, genome editing through CRISPR/Cas9, and QTLs associated with quantity and quality enhancement of cottonseed oil and protein.

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“…Four germplasm lines with elevated levels of seed oil oleic acid were developed and released in 2019 (Dowd et al, 2020). The lines were developed from previously released reniform nematoderesistant germplasm lines that were derived from G. barbadense accession GB713.…”

Section: Germplasm Lines Developed and Releasedmentioning

confidence: 99%

History of USDA-ARS Cotton Host Plant Resistance and Breeding Research at Mississippi State, MS

McCarty¹,

Jenkins²,

Saha³

et al. 2021

JCS

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Cotton host plant resistance research was initiated with the establishment of the Boll Weevil Research Laboratory in 1960. Laboratory objectives were to conduct research and develop technology that ultimately could be used to eradicate the boll weevil. Early research concentrated on developing techniques and screening germplasm for resistance. A full-scale boll weevil eradication trial began in southern Virginia and eastern North Carolina in 1978 and after initial success the USDA Animal and Plant Health Inspection Service established an eradication program. This led the host plant resistance program to broaden its research into other pests of cotton. During the 1980s, research continued to focus on tarnished plant bug, tobacco budworm, expanding the genetic diversity of cotton, basic genetic and cotton breeding studies. With the development of field infestation techniques for the tobacco budworm, in the 1990s the research team conducted the first field test of Bacillus thuringiensis (Bt) transgenic cotton for resistance. During this time, root-knot nematode research expanded. Cotton fruiting efficiency and distribution of harvestable bolls and the concept of plant mapping were developed. During the 2000s, research expanded with the use of chromosome substitution lines for the introgression of new alleles into Upland cotton. Nematode research remained active during this time. To date, the research program has developed and released more than 800 germplasm lines and four random-mating populations. Scientists in the program have trained more than 60 graduate students and countless others have been mentored. The full impact of the research team will only be revealed with time.

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Registration of four upland cotton germplasm lines with elevated levels of seed oil oleic acid

Cited by 8 publications

References 10 publications

Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm

Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm

Genetics, Breeding and Genetic Engineering to Improve Cottonseed Oil and Protein: A Review

History of USDA-ARS Cotton Host Plant Resistance and Breeding Research at Mississippi State, MS

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