Regions of homology in small colicinogenic plasmids

Zverev, Vitaly; Kuzmin, Nikolai P.; Zuyeva, L. A.; Burova, Elena; Alexandrov, A. A.; Khmel, I. A.

doi:10.1016/0147-619x(84)90045-3

Cited by 7 publications

(5 citation statements)

References 5 publications

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“…1) shows a difference from a simple restriction enzyme map presented recently by Zverev et al (35), in which the relative positions of the SmaI fragments B and C are inverted. Since our mapping procedure involved several enzymes which cleave the SmaI fragment B or C or both, we are able to confirm in several different experiments the location of the SmaI sites on ColD (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…ColD is, from this point of view, very similar to ColEl (both plasmids are also dependent on the polA function of the host). This small replication region is contained in a 1,200-base-pair fragment which spans from PvuII (4.3 kb) to EcoRV (0.6 kb) which was shown to contain sequences homologous to the ColEl replication region (35).…”

Section: Discussionmentioning

confidence: 99%

“…Like other colicin plasmids, ColD has been shown to require DNA polymerase I, the host polA gene product, for plasmid replication (15). The fact that ColD requires polA; its small size of 5.12 kb; and the finding that the plasmids ColD, ColA, and ColK have DNA restriction fragments which are homologous to the replication region of ColEl (35) indicate that ColD is similar to several other colicin plasmids (35). However, until now, no extended physical and genetic analysis of the ColD plasmid functions have been made.…”

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confidence: 99%

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Physical and genetic analysis of the ColD plasmid

Frey¹,

Ghersa²,

Palacios³

et al. 1986

J Bacteriol

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The plasmid ColD-CA23, a high-copy-number plasmid of 5.12 kilobases, encodes colicin D, a protein of approximately 87,000 daltons which inhibits bacterial protein synthesis. Colicin D production is under the control of the Escherichia coli SOS regulatory system and is released to the growth medium via the action of the lysis gene product(s). A detailed map of the ColD plasmid was established for 10 restriction enzymes. Using in vitro insertional omega mutagenesis and in vivo insertional Tn5 mutagenesis, we localized the regions of the plasmid responsible for colicin D activity (cda), for mitomycin C-induced lysis (cdl), and for colicin D immunity (cdi). These genes were all located contiguously on a 2,400-base-pair fragment similar to a large number of other Col plasmids (A, E1, E2, E3, E8, N, and CloDF). The ColD plasmid was mobilizable by conjugative transfer by helper plasmids of the IncFII incompatibility group, but not by plasmids belonging to the groups IncI-alpha or IncP. The location of the mobilization functions was determined by deletion analysis. The plasmid needs a segment of 400 base pairs, which is located between the mob genes and the gene for autolysis, for its replication.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Physical and genetic analysis of the ColD plasmid

Frey¹,

Ghersa²,

Palacios³

et al. 1986

J Bacteriol

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“…ColEl is a 6.6-kb E. coli plasmid with a copy number of nearly 20 (6,38). Related multicopy plasmids of members of the family Enterobacteriaceae include plSA, pMB1, RSF1010 (NTP1), CloDF13 (259), NTP16 (158), and other coligenic plasmids (116,196,370,371). Several multicopy cloning vectors such as the well-known plasmids pACYC184 and pBR322 are derived from plSA and pMB1 (13,39).…”

Section: Colel and Related Plasmidsmentioning

confidence: 99%

Replication of plasmids in gram-negative bacteria.

Kües¹,

Ståhl²

1989

Microbiological Reviews

134

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“…The Col plasmid groups such as CoL4, ColD, ColK, and ColEl share a number of replication characteristics. Zverev et al has reported that ColA, ColD, and ColK each contain DNA sequences homologous to the region of ColEl which is involved in autonomous replication [16]. These plasmids are widely used in gene cloning process.…”

Section: Role Of Plasmid In Gene Transfermentioning

confidence: 99%