Regions of bovine adenovirus-3 IVa2 involved in nuclear/nucleolar localization and interaction with pV

Woldemariam, Tekeleselassie Ayalew; Wang, Wenxiu; Said, Abdelrahman; Tikoo, Suresh K.

doi:10.1016/j.virol.2020.04.006

Cited by 4 publications

(20 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The binding of pV to the viral DNA leaves several regions of pV available for binding to other viral proteins, such as pVII and Mu [66,68,75]. A recent report indicated that BAdV-3 pV interacts with a minor core protein, pIVa2, which may help to stabilize the bridge between viral DNA and viral core [82].…”

Section: Functionsmentioning

confidence: 99%

“…The pV linked with each unit of DNA-pVII hexamer [24,68,83] may be helping in the condensation of the viral genome [59]. Moreover, the interaction of pV with pIVa2 (role in DNA encapsidation) has suggested that pV may also play a role in the viral DNA encapsidation [82]. Similarly, the interaction of pV with nucleophosmin 1/NPM 1/B23.1 [84] and pVII [66] has led to speculation about its role in virus assembly and viral mRNA transcription, respectively.…”

Section: Functionsmentioning

confidence: 99%

“…pIVa2 is expressed as 50 kDa, and a 40 kDa truncated protein in HAdV-5 infected cells [91,92]; however, only 50 kDa protein has been detected in BAdV-3 infected cells [82]. The pIVa2 is one of the core proteins [1,93] located interior to the viral capsid and is detected in both the assembly intermediates and mature virions in precursor form [94].…”

Section: Protein Iva2 (Piva2) 231 General Characteristicsmentioning

confidence: 99%

See 2 more Smart Citations

Adenovirus Core Proteins: Structure and Function

Kulanayake

Tikoo

2021

Viruses

View full text Add to dashboard Cite

Adenoviruses have served as a model for investigating viral-cell interactions and discovering different cellular processes, such as RNA splicing and DNA replication. In addition, the development and evaluation of adenoviruses as the viral vectors for vaccination and gene therapy has led to detailed investigations about adenovirus biology, including the structure and function of the adenovirus encoded proteins. While the determination of the structure and function of the viral capsid proteins in adenovirus biology has been the subject of numerous reports, the last few years have seen increased interest in elucidating the structure and function of the adenovirus core proteins. Here, we provide a review of research about the structure and function of the adenovirus core proteins in adenovirus biology.

show abstract

Section: Functionsmentioning

confidence: 99%

Section: Functionsmentioning

confidence: 99%

Section: Protein Iva2 (Piva2) 231 General Characteristicsmentioning

confidence: 99%

See 1 more Smart Citation

Adenovirus Core Proteins: Structure and Function

Kulanayake

Tikoo

2021

Viruses

View full text Add to dashboard Cite

show abstract

“…Members of the importin super family play an important role in the nuclear transport of proteins. Since the transport of some adenovirus proteins requires importins (Kohler et al, 1999;Wodrich et al, 2006;Paterson et al, 2012;Ayalew et al, 2014;Kulshreshtha et al, 2014;Said et al, 2018;Woldemariam et al, 2020), we performed a GST (glutathione-S-transferase) pull down assay using purified GST-fusion proteins of importin α-1, importin α-3, importin α-5, importin α-7, or importin β-1 individually immobilized on glutathione-sepharose beads with radio-labeled in vitro synthesized BAdV-3 pV. As seen in Figure 5A, GST-importin α3 was able to bind pV (lane 5) as a similar protein was observed in input protein control (lane 1).…”

Section: Interaction Of Pv With Importinsmentioning

confidence: 99%

“…Recently, we reported on the characterization of late structural\non-structural BAdV-3 proteins and demonstrated that, while BAdV-3 52K (Paterson et al, 2012), pVIII (Ayalew et al, 2014), 22K (Said et al, 2018), and IVa2 (Woldemariam et al, 2020) utilize the importin α/β pathway to localize to the nucleus in infected cells, BAdV-3 33K utilizes both importin α/β and transportin -SR nuclear import receptor pathways for localization to the nucleus in infected cells (Kulshreshtha et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nuclear and Nucleolar Localization of Bovine Adenovirus-3 Protein V

Zhao

Tikoo

2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The L2 region of bovine adenovirus-3 (BAdV-3) encodes a Mastadenovirus genus-specific protein, designated as pV, which is important for the production of progeny viruses. Here, we demonstrate that BAdV-3 pV, expressed as 55 kDa protein, localizes to the nucleus and specifically targets nucleolus of the infected cells. Analysis of deletion mutants of pV suggested that amino acids 81–120, 190–210, and 380–389 act as multiple nuclear localization signals (NLS), which also appear to serve as the binding sites for importin α-3 protein, a member of the importin α/β nuclear import receptor pathway. Moreover, pV amino acids 21–50 and 380–389 appear to act as nucleolar localization signals (NoLs). Interestingly, amino acids 380–389 appear to act both as NLS and as NoLS. The presence of NoLS is essential for the production of infectious progeny virions, as deletion of both NoLs are lethal for the production of infectious BAdV-3. Analysis of mutant BAV.pVd1d3 (isolated in pV completing CRL cells) containing deletion/mutation of both NoLS in non-complementing CRL cells not only revealed the altered intracellular localization of mutant pV but also reduced the expression of some late proteins. However, it does not appear to affect the incorporation of viral proteins, including mutant pV, in BAV.pVd1d3 virions. Further analysis of CsCl purified BAV.pVd1d3 suggested the presence of thermo-labile virions with disrupted capsids, which appear to affect the infectivity of the progeny virions. Our results suggest that pV contains overlapping and non-overlapping NoLS/NLS. Moreover, the presence of both NoLS appear essential for the production of stable and infectious progeny BAV.pVd1d3 virions.

show abstract

Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins

Kulanayake,

Dar,

Tikoo

2024

Viruses

Self Cite

View full text Add to dashboard Cite

The L 1 region of bovine adenovirus (BAdV)-3 encodes a multifunctional protein named protein VII. Anti-protein VII sera detected a protein of 26 kDa in transfected or BAdV-3-infected cells, which localizes to nucleus and nucleolus of infected/transfected cells. Analysis of mutant protein VII identified four redundant overlapping nuclear/nucleolar localization signals as deletion of all four potential nuclear/nucleolar localization signals localizes protein VII predominantly to the cytoplasm. The nuclear import of protein VII appears to use importin α (α-1), importin-β (β-1) and transportin-3 nuclear transport receptors. In addition, different nuclear transport receptors also require part of protein VII outside nuclear localization sequences for efficient interaction. Proteomic analysis of protein complexes purified from recombinant BAdV-3 expressing protein VII containing Strep Tag II identified potential viral and cellular proteins interacting with protein VII. Here, we confirm that protein VII interacts with IVa2 and protein VIII in BAdV-3-infected cells. Moreover, amino acids 91–101 and 126–137, parts of non-conserved region of protein VII, are required for interaction with IVa2 and protein VIII, respectively.

show abstract

Regions of bovine adenovirus-3 IVa2 involved in nuclear/nucleolar localization and interaction with pV

Cited by 4 publications

References 53 publications

Adenovirus Core Proteins: Structure and Function

Adenovirus Core Proteins: Structure and Function

Nuclear and Nucleolar Localization of Bovine Adenovirus-3 Protein V

Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins

Contact Info

Product

Resources

About