Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of <i>Datura stramonium</i> lectin in glial domains of the optic lobe of the giant prawn

Allodi, Silvana; Bressan, Cristine M.; Carvalho, Sergio L.; Cavalcante, Leny A.

doi:10.1002/glia.20317

Cited by 15 publications

(14 citation statements)

References 73 publications

(91 reference statements)

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“…The shorter process extends towards and contacts the vascular cavity that is located centrally in the niche, terminating in a small endfoot; a long process projects towards the proliferation zone in either Cluster 9 or Cluster 10, forming the streams along which the daughters of the niche precursor cells migrate. The niche cells in the crayfish P. clarkii also label immunocytochemically with an antibody generated against glutamine synthetase, an enzyme that is involved in maintaining the glutamate-glutamine cycle in vertebrate astrocytes (Norenberg and Martinez-Hernandez, 1979; Patel et al, 1985) and which has been used as a glial marker in the brains of vertebrates (Linser, 1985; Hertz and Zielke, 2004; Ari and Kálmán, 2008) and crustaceans (Linser et al, 1997; Allodi et al, 2006; Sullivan et al, 2007a). The niche cells differ from other brain glia in the close proximity of their soma and the fasciculation of their processes into tracts (Sullivan and Beltz, 2007a).…”

Section: Discussionmentioning

confidence: 99%

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Adult neurogenesis in the crayfish brain: Proliferation, migration, and possible origin of precursor cells

Zhang

Allodi

Sandeman

et al. 2009

Developmental Neurobiology

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119

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The birth of new neurons and their incorporation into functional circuits in the adult brain is a characteristic of many vertebrate and invertebrate organisms, including decapod crustaceans. Precursor cells maintaining life-long proliferation in the brains of crayfish (Procambarus clarkii, Cherax destructor) and clawed lobsters (Homarus americanus) reside within a specialized niche on the ventral surface of the brain; their daughters migrate to two proliferation zones along a stream formed by processes of the niche precursors. Here they divide again, finally producing interneurons in the olfactory pathway. The present studies in P. clarkii explore (1) differential proliferative activity among the niche precursor cells with growth and aging, (2) morphological characteristics of cells in the niche and migratory streams, and (3) aspects of the cell cycle in this lineage. Morphologically symmetrical divisions of neuronal precursor cells were observed in the niche near where the migratory streams emerge, as well as in the streams and proliferation zones. The nuclei of migrating cells elongate and undergo shape changes consistent with nucleokinetic movement. LIS1, a highly conserved dynein-binding protein, is expressed in cells in the migratory stream and neurogenic niche, implicating this protein in the translocation of crustacean brain neuronal precursor cells. Symmetrical divisions of the niche precursors and migration of both daughters raised the question of how the niche precursor pool is replenished. We present here preliminary evidence for an association between vascular cells and the niche precursors, which may relate to the life-long growth and maintenance of the crustacean neurogenic niche.

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Section: Discussionmentioning

confidence: 99%

“…The GS antibody served as a glial marker, as reported in previous studies in crustaceans (Linser et al, 1997; Allodi et al, 2006). Monoclonal mouse anti-Elav (1:10; DSHB; No.…”

Section: Methodsmentioning

confidence: 99%

Adult neurogenesis in the crayfish brain: Proliferation, migration, and possible origin of precursor cells

Zhang

Allodi

Sandeman

et al. 2009

Developmental Neurobiology

Self Cite

119

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“…Glutamine synthetase has been used as a reliable marker of glial cells in the vertebrate brain [e.g. [59]] and in crustaceans [56,57,60,61]. However, the neuroimmune role of glia in crustaceans has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

Parasitic manipulation and neuroinflammation: Evidence from the system Microphallus papillorobustus (Trematoda) - Gammarus (Crustacea)

Helluy

Thomas

2010

Parasites Vectors

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BackgroundNeuropathological consequences of neuroinflammatory processes have been implicated in a wide range of diseases affecting the central nervous system (CNS). Glial cells, the resident immune cells of the CNS, respond to tissue injury by releasing proinflammatory cytokines and free radicals such as nitric oxide. We explored the possibility that neuroimmune responses are involved in parasitic manipulation of host behavior in a trematode-crustacean association. The cerebral larva of the flatworm Microphallus papillorobustus alters responses to environmental stimuli - and thus reflex pathways - in the crustacean Gammarus insensibilis, in a way that enhances predation of the crustacean by birds, definitive hosts of the parasite.ResultsImmunocytochemical experiments followed by confocal microscopy were performed to study the distribution of glutamine synthetase, a glial cell marker, and nitric oxide synthase in the brain of gammarids. Astrocyte-like glia and their processes were abundant at the surface of the parasites while levels of nitric oxide synthase were elevated at the host-parasite interface in the brain of gammarids harboring mature cerebral larvae and demonstrating altered behavior.ConclusionTaken together these results lend support to the neuroinflammation hypothesis whereby a chronic CNS specific immune response induced by the parasite plays a role in the disruption of neuromodulation, neuronal integrity, and behavior in infected hosts.

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“…For double labeling of 5-HT 1α or 5- HT 2β receptors and GS, the brains were sequentially incubated first with a mixture of rabbit 5-HT 1crust or 5- HT 2βCrust and mouse anti-GS, and then a mixture of donkey anti-rabbit IgG-Cy2 and donkey anti-mouse IgG-Cy5. The GS antibody served as a glial marker, as reported in previous studies in crustaceans [10,63,64]. …”

Section: Methodsmentioning

confidence: 99%

5-HT receptors mediate lineage-dependent effects of serotonin on adult neurogenesis in Procambarus clarkii

2011

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BackgroundSerotonin (5-HT) is a potent regulator of adult neurogenesis in the crustacean brain, as in the vertebrate brain. However, there are relatively few data regarding the mechanisms of serotonin's action and which precursor cells are targeted. Therefore, we exploited the spatial separation of the neuronal precursor lineage that generates adult-born neurons in the crayfish (Procambarus clarkii) brain to determine which generation(s) is influenced by serotonin, and to identify and localize serotonin receptor subtypes underlying these effects.ResultsRT-PCR shows that mRNAs of serotonin receptors homologous to mammalian subtypes 1A and 2B are expressed in P. clarkii brain (referred to here as 5-HT1α and 5-HT2β). In situ hybridization with antisense riboprobes reveals strong expression of these mRNAs in several brain regions, including cell clusters 9 and 10 where adult-born neurons reside. Antibodies generated against the crustacean forms of these receptors do not bind to the primary neuronal precursors (stem cells) in the neurogenic niche or their daughters as they migrate, but do label these second-generation precursors as they approach the proliferation zones of cell clusters 9 and 10. Like serotonin, administration of the P. clarkii 5-HT1α-specific agonist quipazine maleate salt (QMS) increases the number of bromodeoxyuridine (BrdU)-labeled cells in cluster 10; the P. clarkii 5-HT2β-specific antagonist methiothepin mesylate salt (MMS) suppresses neurogenesis in this region. However, serotonin, QMS and MMS do not alter the rate of BrdU incorporation into niche precursors or their migratory daughters.ConclusionOur results demonstrate that the influences of serotonin on adult neurogenesis in the crayfish brain are confined to the late second-generation precursors and their descendants. Further, the distribution of 5-HT1α and 5-HT2β mRNAs and proteins indicate that these serotonergic effects are exerted directly on specific generations of neuronal precursors. Taken together, these results suggest that the influence of serotonin on adult neurogenesis in the crustacean brain is lineage dependent, and that 5-HT1α and 5-HT2β receptors underlie these effects.

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Regionally specific distribution of the binding of anti‐glutamine synthetase and anti‐S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

Cited by 15 publications

References 73 publications

Adult neurogenesis in the crayfish brain: Proliferation, migration, and possible origin of precursor cells

Adult neurogenesis in the crayfish brain: Proliferation, migration, and possible origin of precursor cells

Parasitic manipulation and neuroinflammation: Evidence from the system Microphallus papillorobustus (Trematoda) - Gammarus (Crustacea)

5-HT receptors mediate lineage-dependent effects of serotonin on adult neurogenesis in Procambarus clarkii

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