Regionalized quantitative LA-ICP-MS imaging of the biodegradation of magnesium alloys in bone tissue

Draxler, Johannes; Zitek, Andreas; Meischel, Martin; Stranzl-Tschegg, Stefanie E.; Mingler, Bernhard; Martinelli, Elisabeth; Weinberg, Annelie M.; Prohaska, Thomas

doi:10.1039/c5ja00354g

Cited by 17 publications

(8 citation statements)

References 34 publications

(57 reference statements)

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“…With advances in Mg alloy research, many authors have suggested the importance of the quantitative determination of the spatial Mg accumulation for understanding the degradation process and the interaction between Mg release and biological responses [ 9 ]. However, no studies were focused on intervertebral Mg accumulation as most of the previous studies were performed for Mg-based screws [ 6 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of near-implant magnesium accumulation for a Si-containing coated AZ31 cage from a goat cervical spine fusion model

Zhang

Wang

et al. 2018

BMC Musculoskelet Disord

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BackgroundMagnesium (Mg) released from Mg-based implants degradation is believed to be effective in improving osteogenesis, however, studies focusing on Mg-based interbody cages are limited and fusion success was never reported. As excessive Mg accumulation can inhibit new bone formation, this study is designed to explain the possible reasons for the fusion failure of Mg-based cages by analyzing the relationships between the intervertebral Mg accumulation and the resulting interbody fusion.MethodsThe experimental cage was consisted of magnesium alloy (AZ31) substrate and Silicon (Si) -containing coating. C3/C4 and C5/C6 of 24 goats were implanted with cage or autologous iliac crest bone graft (Control group), which were analyzed at 3, 6, 12, and 24 weeks post-operatively. Intervertebral Mg concentrations, Mg-related Calcium (Ca)/ Phosphorus (P) ratios, radiological evaluations and histological findings were recorded for analyzing the relationships between the three of cage corrosion, Mg accumulation, and interbody fusion.ResultsIntervertebral Mg levels were significantly increased after cage implantation, especially in the areas that were closer to the cages at 3 weeks post-operatively, and these increased concentrations could persist up to 12 weeks post-operatively, indicating a relatively rapid corrosion process. Significantly lower Mg levels were only found at 24 weeks post-operatively, but these levels were still higher than those of the control group. In addition, Mg was found to be widely distributed at the intervertebral space since high Mg concentrations could even be detected at the posterior boundary of the vertebral body. Under this Mg accumulation profile, interbody fusion was not achieved, as indicated by the decreased Ca/P ratios, low CT fusion scores and negative histological results.ConclusionsIntervertebral excessive Mg accumulation might be the primary reason for interbody fusion failure. Quantitative Mg analysis can offer insight into the association between cage degeneration and biological response.

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Section: Introductionmentioning

confidence: 99%

Quantitative analysis of near-implant magnesium accumulation for a Si-containing coated AZ31 cage from a goat cervical spine fusion model

Zhang

Wang

et al. 2018

BMC Musculoskelet Disord

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“…The LA ICP-QMS measurements to detect the spatial distribution of 138 Ba/ 43 Ca ratios of the thin sections of the i2s from ind1, ind2 and ind3 were performed using a nanosecond excimer-based laser ablation system (NWR 193, ESI-NWR Division, Electro Scientific Industries, Inc., Portland, CA, USA) coupled to an ICP-QMS (NexION 350D, Perkin Elmer, Waltham, MA, USA) similar to the protocol of Draxler et al 90 . Further multi-elemental distributions were monitored to define the tooth tissue ( 31 P) and to identify diagenetic alterations ( 27 Al, 57 Fe, 88 Sr).…”

Section: Methodsmentioning

confidence: 99%

“…The limit of detection (LOD) was calculated as 3× standard deviation (SD) of this blank signal. Data reduction was performed with an in-house developed MS Excel Macro 90 . All blank corrected values smaller than the LOD were set to zero.…”

Section: Methodsmentioning

confidence: 99%

Ancient DNA reveals monozygotic newborn twins from the Upper Palaeolithic

et al. 2020

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The Upper Palaeolithic double burial of newborns and the single burial of a ca. 3-month-old infant uncovered at the Gravettian site of Krems-Wachtberg, Austria, are of paramount importance given the rarity of immature human remains from this time. Genome-wide ancient DNA shows that the male infants of the double grave are the earliest reported case of monozygotic twins, while the single grave´s individual was their 3rd-degree male relative. We assessed the individuals´ age at death by applying histological and µCT inspection of the maxillary second incisors (i2) in conjunction with C- and N-isotope ratios and Barium (Ba) intake as biomarker for breastfeeding. The results show that the twins were full-term newborns, and that while individual 2 died at birth, individual 1 survived for about 50 days. The findings show that Gravettian mortuary behaviour also included re-opening of a grave and manipulation of its layout and content.

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“…These data were exported from ArcGIS® and imported into Microso® Excel where the coordinates between the start and endpoint were calculated for each data point of the time resolved line scans. 25 The line scan data equipped with x-y coordinates were re-imported into ArcGIS® and displayed as a direct overlay of the otolith image. In consecutive steps outlier detection, 3-D visualisation and data integration were performed directly in ArcGIS®.…”

Section: Spatial Data Referencing and Statistical Analysismentioning

confidence: 99%

“…successfully been applied and published recently by ref. 25. In this work, the further development and application of imaging laser ablation split stream ICP-MS/MC ICP-MS is presented for the analysis of the elemental and Sr isotopic distributions in a sh otolith sample for the rst time.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous multi-element and isotope ratio imaging of fish otoliths by laser ablation split stream ICP-MS/MC ICP-MS

Prohaska

Irrgeher

Zitek

2016

J. Anal. At. Spectrom.

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Regionalized quantitative LA-ICP-MS imaging of the biodegradation of magnesium alloys in bone tissue

Abstract: LA-ICP-MS data were spatially referenced to the respective microscope images to investigate the biodegradation of Mg alloys in bone tissue.

Cited by 17 publications

References 34 publications

Quantitative analysis of near-implant magnesium accumulation for a Si-containing coated AZ31 cage from a goat cervical spine fusion model

Quantitative analysis of near-implant magnesium accumulation for a Si-containing coated AZ31 cage from a goat cervical spine fusion model

Ancient DNA reveals monozygotic newborn twins from the Upper Palaeolithic

Simultaneous multi-element and isotope ratio imaging of fish otoliths by laser ablation split stream ICP-MS/MC ICP-MS

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