Regional Variation in the Lamina Propria T Cell Receptor Vβ Repertoire in Normal Human Colon

Bennet, Justin D.; Brown, William R.; Kotzin, Brian L.

doi:10.1006/clim.1998.4627

Cited by 10 publications

(4 citation statements)

References 39 publications

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“…18,21,22,26,29,30 However, a much faster and more quantitative analysis of the TCR␤ repertoire is now possible through the use of mAbs directed against the V␤ domains of TCR␣␤ molecules, which cover 65% to 70% of V␤ domains in blood T lymphocytes of children and adults. 10,[31][32][33][34] We performed parallel molecular and flow cytometric V␤ analyses in a series of SB-defined TCR␣␤ T-ALL samples and T-cell lines to validate both approaches for the detection of clonality. Indeed, clonal V␤-C␤ transcripts could easily be detected in all T-ALL samples and T-cell lines.…”

Section: V␤ Repertoire Analysis For Identification Of Clonal Cell Popmentioning

confidence: 99%

Molecular and flow cytometric analysis of the Vβ repertoire for clonality assessment in mature TCRαβ T-cell proliferations

Langerak

Beemd

Wolvers-Tettero

et al. 2001

Blood

218

148

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Section: V␤ Repertoire Analysis For Identification Of Clonal Cell Popmentioning

confidence: 99%

Molecular and flow cytometric analysis of the Vβ repertoire for clonality assessment in mature TCRαβ T-cell proliferations

Langerak

Beemd

Wolvers-Tettero

et al. 2001

Blood

218

148

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“…Using this method, repertoire differences between blood and other anatomical sites can be investigated. For instance, large differences in T cell repertoire profile have been observed between peripheral blood mononuclear cells (PBMC) and colon tissue (2), with colon-resident T cells expressing a much more restricted TRBV signature (3). Compartment-specific T cell repertoires have also been noted at the fetal–maternal interface (4).…”

Section: Introductionmentioning

confidence: 99%

Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood

et al. 2014

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The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and pre-eclampsia. We found total T cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the fetal–maternal interface. Defining the functional nature and specificity of compartment-specific T cells will be necessary if we are to understand localized immunity, tolerance, and pathogenesis.

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“…Recently, Bennett et al (24) have investigated the clonality of LPLs in the human colon. They chose to use antibody staining of isolated cells from 13 patients without IBD.…”

Section: Intestinal αβTcr Repertoire In Healthmentioning

confidence: 99%

Repertoire of the αβ T‐cell receptor in the intestine

Probert

Saubermann

Balk

et al. 2007

Immunological Reviews

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The majority of T cells in the human and mouse intestine express the T-cell receptor (TCR) as an alphabeta heterodimer on their cell surface. As the major recognition element of antigens in the context of major histocompatibility complex-derived proteins, an examination of the structure of the alpha beta TCR in intestines has provided significant insights into the potential function of these cells and the major determinants that drive their selection. Studies in the human intestine have shown that the repertoires of intraepithelial lymphocytes (IELs), and likely lamina propria lymphocytes, are polyclonal before and shortly after birth, with the repertoire becoming oligoclonal in adults. Similarly, in adult mice the repertoire is oligoclonal, while in the newborn it is polyclonal. Investigations in mice have shown that some T cells may evade thymic selection. The population size and oligoclonality of IELs is influenced by the microbial content of the luminal microenvironment. This microenvironment probably directly determines the TCR repertoire. Studies in human inflammatory bowel disease (IBD) indicate that inflammation further skews the TCR repertoire. We speculate that dominant antigens associated with the pathogenesis of IBD are responsible for such skewing and that identifying the antigenic drivers may shed light on the environmental factors that trigger or potentiate human IBD.

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Regional Variation in the Lamina Propria T Cell Receptor Vβ Repertoire in Normal Human Colon

Cited by 10 publications

References 39 publications

Molecular and flow cytometric analysis of the Vβ repertoire for clonality assessment in mature TCRαβ T-cell proliferations

Molecular and flow cytometric analysis of the Vβ repertoire for clonality assessment in mature TCRαβ T-cell proliferations

Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood

Repertoire of the αβ T‐cell receptor in the intestine

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