Regional regenerative potential of meniscal cartilage exposed to recombinant insulin-like growth factor-I <i>in vitro</i>

Tumia, Nezar; Johnstone, Alan J.

doi:10.1302/0301-620x.86b7.13747

Cited by 38 publications

(23 citation statements)

References 35 publications

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“…Furthermore, census data indicates a significant increase in the longevity of the general population; thus, the prevalence of meniscal disorders will therefore increase in the forthcoming decades requiring effective solutions to such musculoskeletal conditions. Modern approaches using tissue engineering (Peretti et al 2004;Buma et al 2004;Tumia and Johnstone 2004b) and novel biological agents to stimulate meniscal repair may represent possible therapeutic intervention strategies worthy of further evaluation for the treatment of such problematic clinical disorders. …”

Section: Discussionmentioning

confidence: 99%

Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study

Melrose¹,

Smith²,

Cake

et al. 2005

Histochem Cell Biol

127

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This is the first study to immunolocalise perlecan in meniscal tissues and to demonstrate how its localisation varied with ageing relative to aggrecan and type I, II and IV collagen. Perlecan was present in the middle and inner meniscal zones where it was expressed by cells of an oval or rounded morphology. Unlike the other components visualised in this study, perlecan was strongly cell associated and its levels fell significantly with age onset and cell number decline. The peripheral outer meniscal zones displayed very little perlecan staining other than in small blood vessels. Picrosirius red staining viewed under polarised light strongly delineated complex arrangements of slender discrete randomly oriented collagen fibre bundles as well as transverse, thick, strongly oriented, collagen tie bundles in the middle and outer meniscal zones. The collagen fibres demarcated areas of the meniscus which were rich in anionic toluidine blue positive proteoglycans; immunolocalisations confirmed the presence of aggrecan and perlecan. When meniscal sections were examined macroscopically, type II collagen localisation in the inner meniscal zone was readily evident in the 2- to 7-day-old specimens; this became more disperse in the older meniscal specimens. Type I collagen had a widespread distribution in all meniscal zones at all time points. Type IV collagen was strongly associated with blood vessels in the 2- to 7-day-old meniscal specimens but was virtually undetectable at the later time points (>7 month).

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Section: Discussionmentioning

confidence: 99%

Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study

Melrose¹,

Smith²,

Cake

et al. 2005

Histochem Cell Biol

127

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“…Based on the blood and nutrition supply, the meniscus is divided into three zones: a red zone, a white zone, and a red-white zone [24]. Fibrochondrocytes in different zones of the menisci display different morphology correlating with their stress and nutritional microenvironment [28]. In primary monolayer cell cultures, the meniscal fibrochondrocytes are a mixture of phenotypes, oval-or spindle-shaped cells that normally exist in the superficial layer of the meniscus, and roundshaped cells which are found predominantly in the deep layer.…”

Section: Introductionmentioning

confidence: 99%

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Zhang

Leng

Wang

et al. 2009

Clin Orthop Relat Res

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The menisci are intraarticular fibrocartilaginous structures essential to the normal function of the knee that lack the ability to self-repair. Human meniscal fibrochondrocytes may respond to beneficial genes like human insulin growth factor-1 (hIGF-1) and the meniscal cell may be a feasible donor for gene therapy. To explore this possibility, we amplified the hIGF-1 gene sequence in full length and cloned it into a bicistronic plasmid. This gene was then transfected into cultured human meniscal fibrochondrocytes by the liposome FuGene 6. Green fluorescence was expressed in part of the cells 6 hours after transfection and increased gradually, with a peak concentration of the hIGF-1 in the supernatants to 22.68 ng/mL 56 hours after transfection. Phenotypes of some cells changed and the proliferation accelerated after transfection. Flow cytometry analysis demonstrated upregulation of cell numbers in the G2 and S stages after hIGF-1 gene introduction. We conclude the hIGF-1 gene can be transfected into the human meniscal cell efficiently by liposome and it causes accelerated proliferation and differentiation. Within 10 days after transfection, the cytokine appears to be secreted into supernatants with the bioactivity and promotes the proliferation of the NIH 3T3 cell line.

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“…Tumia and colleagues presented a series of experiments in which they showed that meniscal cells from the inner avascular zone could be stimulated to proliferate when exposed to IGF [41], FGF [42], and PDGF-AB [43]. Platelet derived growth factor, in particular was shown to stimulate an 8-fold increase in cell proliferation and a 4-fold increase in matrix production in vitro [43].…”

Section: Cell Biology and Meniscal Healing: Cytokines And Growth Factorsmentioning

confidence: 99%

Augmentation techniques for isolated meniscal tears

Taylor

Rodeo

2013

Curr Rev Musculoskelet Med

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Meniscal tears are relatively common injuries sustained by athletes and non-athletes alike and have far reaching functional and financial implications. Studies have clearly demonstrated the important biomechanical role played by the meniscus. Long-term follow-up studies of post-menisectomy patients show a predisposition toward the development of degenerative arthritic changes. As such, substantial efforts have been made by researchers and clinicians to understand the cellular and molecular basis of meniscal healing. Proinflammatory cytokines have been shown to have a catabolic effect on meniscal healing. In vitro and some limited in vivo studies have shown a proliferative and anabolic response to various growth factors. Surgical techniques that have been developed to stimulate a healing response include mechanical abrasion, fibrin clot application, growth factor application, and attempts at meniscal neovascularization. This article discusses various augmentation techniques for meniscal repair and reviews the current literature with regard to fibrin clot, platelet rich plasma, proinflammatory cytokines, and application of growth factors.

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Regional regenerative potential of meniscal cartilage exposed to recombinant insulin-like growth factor-I in vitro

Cited by 38 publications

References 35 publications

Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study

Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Augmentation techniques for isolated meniscal tears

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