Regional Occurrence of Plasmid-Mediated Carbapenem-Hydrolyzing Oxacillinase OXA-58 in
            <i>Acinetobacter</i>
            spp. in Europe

Marqué, Sophie; Poirel, Laurent; Héritier, Claire; Brisse, Sylvain; Blasco, Maria Dolores; Filip, Roxana; Coman, Gabriela; Naas, Thierry; Nordmann, Patrice

doi:10.1128/jcm.43.9.4885-4888.2005

Cited by 92 publications

(97 citation statements)

References 21 publications

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“…Our results support previous reports indicating the worldwide spread of the bla OXA-58 gene and highlight its importance in conferring carbapenem resistance among A. baumannii isolates from Latin America Higgins et al, 2010a;Kulah et al, 2010;Marqué et al, 2005;McCracken et al, 2009;Mendes et al, 2009;Papa et al, 2009). …”

Section: E Sevillano and Others 82supporting

confidence: 82%

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Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Sevillano

Fernández

Bustamante

et al. 2012

Journal of Medical Microbiology

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and the presence of carbapenemases as a mechanism of resistance to imipenem. Isolates were obtained from 46 patients (one isolate per patient; 30 males,16 females) with an age range of 1 day to 84 years, and were collected from different sample types, the majority from respiratory tract infections (17) and wounds (13). Resistance to imipenem was detected in 15 isolates collected from different hospitals of the city. These isolates grouped into the same genotype, named A, and were resistant to all antibiotics tested including imipenem, with susceptibility only to colistin. Experiments to detect carbapenemases revealed the presence of the OXA-58 carbapenemase. Further analysis revealed the location of the bla OXA-58 gene on a 40 kb plasmid. To our knowledge, this is the first report of carbapenem resistance in A. baumannii isolates from Bolivia that is conferred by the OXA-58 carbapenemase. The presence of this gene in a multidrug-resistant clone and its location within a plasmid is of great concern with regard to the spread of carbapenem-resistant A. baumannii in the hospital environment in Bolivia. INTRODUCTIONAcinetobacter baumannii is a nosocomial pathogen responsible for severe infections often diagnosed in patients attending hospitals worldwide. Carbapemens are considered the drugs of choice for treating these infections, but they are not always efficient due to the occurrence of multidrug-resistant strains of this bacterium (Canduela et al., 2006;Coelho et al., 2006;Higgins et al., 2010a;Mendes et al., 2009;Poirel et al., 2010). Mechanisms enabling the development of carbapenem resistance include an induced decrease in membrane permeability, efflux pump overexpression and the production of carbapenemases. Among these mechanisms, the production of carbapenemases plays a major role in carbapenem resistance in most Gram-negative bacilli, including A. baumannii clinical isolates (Canduela et al., 2006;Coelho et al., 2006;Poirel et al., 2010;Queenan & Bush, 2007;Walsh, 2010). The most common type of enzyme occurring in A. baumannii is represented by the carbapenem-hydrolysing class D b-lactamases, which are divided into five phylogenetic groups: OXA-23-like, OXA-24-like, OXA-58-like, OXA-51-like, which is intrinsic to A. baumannii, and the recently described OXA-143-like enzymes (Higgins et al., 2009;Queenan & Bush, 2007).Although A. baumannii is considered an emerging pathogen and much research has been carried out on it, there have been only a few studies concerning the mechanisms conferring carbapenem resistance of this bacterium in Latin American countries (Celenza et al., 2006;Coelho et al., 2006;Sader et al., 2004;Sgambatti et al., 2010). In addition, there are no previous publications regarding the resistance of A. baumannii to imipenem or the occurrence of carbapenemases in A. baumannii isolates in Bolivia.In this study, we analysed the antibiotic resistance, in particular to imipenem, of clinical isolates of A. baumannii obtained from several hospitals in Cochabamba, Bolivia, and the presence of carbape...

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Section: E Sevillano and Others 82supporting

confidence: 82%

“…Southern blot analysis with a bla OXA-58 -specific probe generated by PCR enabled us to localize this gene on the 40 kb plasmid. This plasmid was within the size range (10-150 kb) described by other authors (Bertini et al, 2006;Héritier et al, 2005;Marqué et al, 2005;Poirel et al, 2005;Wang et al, 2007).…”

Section: E Sevillano and Others 82mentioning

confidence: 80%

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Sevillano

Fernández

Bustamante

et al. 2012

Journal of Medical Microbiology

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“…Isolates had been identified by using the API32GN systems (bioMérieux, Marcy-l'Etoile, France). In this study, we have selected a single representant of each pulsotype as previously determined (16). In addition, strains belonging to the identical pulsotype but possessing different plasmids were also studied (Table 1).…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…The bla OXA-58 gene was searched for by using a PCR technique with primers OXA-58A and OXA-58B as described previously (16). In addition, two bla OXA-58 -negative A. baumannii isolates not listed in Table 1 were included.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…The bla OXA-58 gene was found to be plasmid located in most cases. This oxacillinase gene has been identified in a carbapenem-resistant A. baumannii isolate that was at the origin of a nosocomial outbreak in Toulouse, France (9), and further studies showed that carbapenemase OXA-58 was very widespread, since it has been detected in A. baumannii isolates recovered from different countries from southern Europe, the Balkans, and central Turkey (16) and also from Argentina, Kuwait, and the United Kingdom (J. Coelho, N. Woodford, M. Afzal-Shah, and D. Livermore, 6th Int. Symp.…”

mentioning

confidence: 99%

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Acinetobacter baumannii

2015

Reviews in Medical Microbiology

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Genetic structures surrounding the carbapenem-hydrolyzing oxacillinase gene bla OXA-58 were characterized in a series of OXA-58-positive Acinetobacter baumannii strains isolated from different countries. We showed that in most of the cases, acquisitions of the bla OXA-58 -containing overall structure, including insertion sequence elements, may be likely the results of recombination events. In type strain A. baumannii MAD, the genetic structure surrounding the bla OXA-58 gene was bracketed by two 27-bp repeated sequences. The isolation of a clonally related OXA-58-negative A. baumannii isolate that possessed the same plasmid backbone as A. baumannii MAD but lacked this genetic structure indicated that the mechanism of acquisition could be reversible. Parts of the structure identified in A. baumannii MAD were conserved in other bla OXA-58 -positive isolates from various European countries. Primer extension experiments showed that bla OXA-58 expression was related to promoter sequences brought by different insertion sequence elements, such as an ISAba3-like element, ISAba1, ISAba2, and IS18. This work identified novel structures at the origin of acquisition and expression of a carbapenem-hydrolyzing ␤-lactamase identified in non-clonally related A. baumannii isolates.

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Acinetobacter baumannii: Mechanisms of Resistance, Multiple ß-Lactamases

Poirel

Nordmann

2008

Acinetobacter Biology and Pathogenesis

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Regional Occurrence of Plasmid-Mediated Carbapenem-Hydrolyzing Oxacillinase OXA-58 in Acinetobacter spp. in Europe

Cited by 92 publications

References 21 publications

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Acinetobacter baumannii

Acinetobacter baumannii: Mechanisms of Resistance, Multiple ß-Lactamases

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