Regional localization ofCCG1 gene which complements hamster cell cycle mutation BN462 to Xq11?Xq13

Brown, Carolyn J.; Sekiguchi, Toshio; Nishimoto, Tetsuya; Willard, Huntington F.

doi:10.1007/bf01534674

Cited by 17 publications

(5 citation statements)

References 24 publications

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“…The contig was initially constructed using previously mapped loci within the region IL2R␥, AFX1, DXS7117, GJB1, DXS6673E, p54 nrb , CCG1, DXS7120, and DXS559 (Brown et al, 1989;Bergoffen et al, 1993;Dong et al, 1993;Nakashima et al, 1994;Haberhausen et al, 1995 Peters et al, 1997). End clones from PACs 193D5, 548P16, 423F18, 104C1 and the left end of YAC WXD945 were isolated, sequenced, and used as STSs to complete the contig (see below and Table 4).…”

Section: Methodsmentioning

confidence: 99%

Refined Linkage Disequilibrium and Physical Mapping of the Gene Locus for X-Linked Dystonia–Parkinsonism (DYT3)

et al. 1999

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Section: Methodsmentioning

confidence: 99%

Refined Linkage Disequilibrium and Physical Mapping of the Gene Locus for X-Linked Dystonia–Parkinsonism (DYT3)

et al. 1999

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“…The isolated nuclei were suspended in buffer A, which contained 10 mM Tris-HCl (pH 7.5), 1 mM MgCl2, 5 ,ug each of aprotinin, pepstatin, and leupeptin per ml, and 1 mM phenylmethylsulfonyl fluoride. The isolated nuclei were suspended in NaCl at a final concentration of 0.4 M. The salt-extracted proteins and the residual proteins were separated by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

“…But once it enters the S phase, DNA replication progresses normally. By using this mutant as the recipient of the DNA-mediated gene transfer, a human gene located between qll and q13 on the X chromosome has been cloned and designated the cell cycle gene 1 (CCGI) (5,31,32). Its cDNA complements tsi3 in addition to tsBN462, both of which were independently isolated from BHK21 cells and belong to the same comple-* Corresponding author.…”

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confidence: 99%

The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

et al. 1991

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The human CCGI gene complements tsBN462, a temperature-sensitive G1 mutant of the BHK21 cell line. The previously cloned cDNA turned out to be a truncated form of the actual CCGI cDNA. The newly cloned CCGI cDNA was 6.0 kb and encoded a protein with a molecular mass of 210 kDa. Using an antibody to a predicted peptide from the CCG1 protein, a protein with a molecular mass of over 200 kDa was identified in human, monkey, and hamster cell lines. In the newly defined C-terminal region, an acidic domain was found. It contained four consensus target sequences for casein kinase II and was phosphorylated by this enzyme in vitro. However, this C-terminal region was not required to complement tsBN462 mutation since the region encoding the C-terminal part was frequently missing in complemented clones derived by DNA-mediated gene transfer. CCG1 contains a sequence similar to the putative DNA-binding domain of HMG1 in addition to the previously detected amino acid sequences common in nuclear proteins, such as a proline cluster and a nuclear translocation signal. Consistent with these predictions, CCG1 was present in nuclei, possessed DNA-binding activity, and was eluted with similar concentrations of salt, 0.3 to 0.4 M NaCl either from isolated nuclei or from a DNA-cellulose column.The cell cycle of eucaryotic cells is composed of the consecutive phases Gl, S, G2, and M. In the G, phase, depending on the stimulus of growth factors, protein and RNA molecules are produced and the new cell cycle initiates (25). Unresolved problems concerning the start of the cell cycle include how the stimulus of external growth factors reaches the nucleus and how cells recognize the accumulation of materials required to enter the new cell cycle. To investigate the initiation of the cell cycle at the molecular level, it is essential to identify the genes involved. In both budding and fission yeasts, various temperature-sensitive (ts) mutants defective in the progression of the cell cycle have been isolated and have proven to be most useful for identifying the genes required for cell cycle progression (12,22). Various ts+ cell cycle mutants have also been isolated from cultured animal cells (3). The FT210 cell line, a ts G2 mutant of FM3A, has a ts cdc2 gene product; thus, the cdc2 gene is apparently essential for the cell cycle of animal cells (36).We isolated ts cell cycle mutants from the BHK21 cell line derived from the Syrian hamster and then classified them into complementation groups (21). One of these ts mutants, tsBN462, has a defect in progression of the G1 phase, which is that after release from the G, block, it is unable to enter the S phase at a nonpermissive temperature. But once it enters the S phase, DNA replication progresses normally. By using this mutant as the recipient of the DNA-mediated gene transfer, a human gene located between qll and q13 on the X chromosome has been cloned and designated the cell cycle gene 1 (CCGI) (5,31,32). Its cDNA complements tsi3 in addition to tsBN462, both of which were independently isolated ...

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“…In previous studies we isolated the CCG1 gene, which complements the tsBN462 mutation (Sekiguchi et al 1988(Sekiguchi et al , 1991. The CCG1 gene maps to human chromosome X q11-q13 (Brown et al 1989), and in both tsBN462 and ts13 cells there is the same point mutation in CCG1, resulting in the change of Gly 690 to Asp 690 (Hayashida et al 1994).…”

Section: Introductionmentioning

confidence: 99%

D‐type cyclin expression is decreased and p21 and p27 CDK inhibitor expression is increased when tsBN462 CCG1/TAF_II250 mutant cells arrest in G1 at the restrictive temperature

et al. 1996

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Regional localization ofCCG1 gene which complements hamster cell cycle mutation BN462 to Xq11?Xq13

Cited by 17 publications

References 24 publications

Refined Linkage Disequilibrium and Physical Mapping of the Gene Locus for X-Linked Dystonia–Parkinsonism (DYT3)

Refined Linkage Disequilibrium and Physical Mapping of the Gene Locus for X-Linked Dystonia–Parkinsonism (DYT3)

The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

D‐type cyclin expression is decreased and p21 and p27 CDK inhibitor expression is increased when tsBN462 CCG1/TAF_II250 mutant cells arrest in G1 at the restrictive temperature

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Regional localization ofCCG1 gene which complements hamster cell cycle mutation BN462 to Xq11?Xq13

Cited by 17 publications

References 24 publications

Refined Linkage Disequilibrium and Physical Mapping of the Gene Locus for X-Linked Dystonia–Parkinsonism (DYT3)

Refined Linkage Disequilibrium and Physical Mapping of the Gene Locus for X-Linked Dystonia–Parkinsonism (DYT3)

The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.

D‐type cyclin expression is decreased and p21 and p27 CDK inhibitor expression is increased when tsBN462 CCG1/TAFII250 mutant cells arrest in G1 at the restrictive temperature

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D‐type cyclin expression is decreased and p21 and p27 CDK inhibitor expression is increased when tsBN462 CCG1/TAF_II250 mutant cells arrest in G1 at the restrictive temperature