Regional heterogeneity of substance P-induced endothelium-dependent contraction, relaxation, and -independent contraction in rabbit pulmonary arteries

Miike, Tomohiro; Shirahase, Hiroaki; Kanda, Mamoru; Kunishiro, Kazuyoshi; Kurahashi, Kazuyoshi

doi:10.1016/j.lfs.2008.09.025

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…That different gene assemblies were altered in different brain regions in the present study may imply that the same “physiologic endpoint” – reduced angiogenic potential – can be achieved through diverse manipulation of multiple angiogenesis-associated genes (Menon, et al, 2011), and that brain region is a decisive factor in the gene selection process. Such regional control over angiogenic potential is consistent with both asymmetrical brain aging (Berchtold et al, 2008) and endothelial heterogeneity (Aird, 2008; Chi, et al, 2003; Miike, et al, 2008). …”

Section: 0 Discussionsupporting

confidence: 54%

Brain regional angiogenic potential at the neurovascular unit during normal aging

Murugesan

Demarest

Madri

et al. 2012

Neurobiology of Aging

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Given strong regional specialization of the brain, cerebral angiogenesis may be regionally modified during normal aging. To test this hypothesis, expression of a broad cadre of angiogenesis-associated genes was assayed at the neurovascular unit (NVU) in discrete brain regions of young vs. aged mice by laser capture microdissection coupled to quantitative real-time PCR. Complementary quantitative capillary density/branching studies were performed as well. Effects of physical exercise were also assayed to determine if age-related trends could be reversed. Additionally, gene response to hypoxia was probed to highlight age-associated weaknesses in adapting to this angiogenic stress. Aging impacted resting expression of angiogenesis-associated genes at the NVU in a region-dependent manner. Physical exercise reversed some of these age-associated gene trends, as well as positively influenced cerebral capillary density/branching in a region-dependent way. Lastly, hypoxia revealed a weaker angiogenic response in aged brain. These results suggest heterogeneous changes in angiogenic capacity of the brain during normal aging, and imply a therapeutic benefit of physical exercise that acts at the level of the NVU.

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Section: 0 Discussionsupporting

confidence: 54%

Brain regional angiogenic potential at the neurovascular unit during normal aging

Murugesan

Demarest

Madri

et al. 2012

Neurobiology of Aging

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“…The segmental differential responses to vasoactive substances may have important implications for the regulation of resistance in this low-tone vascular bed (35). Various studies including ours demonstrate that the reactivities of conduit pulmonary vessels are actively regulated and may play a substantial role in the regulation of pulmonary circulation (9,11,(15)(16)(17)(18)(19)41).…”

Section: Discussionmentioning

confidence: 93%

Long-term effects of prenatal hypoxia on endothelium-dependent relaxation responses in pulmonary arteries of adult sheep

Gao

Negash

Longo

et al. 2009

American Journal of Physiology-Lung Cellular and Molecular Phys

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Chronic hypoxia during the course of pregnancy is a common insult to the fetus. However, its long-term effect on the pulmonary vasculature in adulthood has not been described. In this study, the vasorelaxation responses of conduit pulmonary arteries in adult female sheep that were chronically hypoxic as fetuses and raised postnatally at sea level were investigated. Vessel tension studies revealed that endothelium-dependent relaxation responses were attenuated in pulmonary arteries from adult sheep that experienced prenatal hypoxia. Endothelial nitric oxide synthase (eNOS) protein expression was unchanged, but eNOS activity was significantly decreased in pulmonary arteries from prenatally hypoxic sheep. Protein expression of eNOS partners, caveolin-1, calmodulin, and heat shock protein 90 (Hsp90) did not change following prenatal hypoxia. However, the association between eNOS and caveolin-1, its inhibitory binding partner, was significantly increased, whereas association between eNOS and its stimulatory partners calmodulin and Hsp90 was greatly decreased. Furthermore, phosphorylation of Ser(1177) in eNOS decreased, whereas phosphorylation of Thr(495) increased, in the prenatally hypoxic pulmonary arteries, events that are related to eNOS activity. These data demonstrate that prenatal hypoxia results in persistent abnormalities in endothelium-dependent relaxation responses of pulmonary arteries in adult sheep due to decreased eNOS activity resulting from altered posttranslational regulation.

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“…The signature domain of TSP1, E123CaG1, that specifically binds CD47 also inhibited ACh-stimulated vasorelaxation ( Figure 3B and Table 1). Ionomycin, through activating eNOS, can stimulate arterial relaxation 21 and TSP1 also inhibited ionomycin-stimulated vasorelaxation ( Figure 3C).…”

Section: Soluble Tsp1 Inhibits Enos-dependent Arterial Relaxationmentioning

confidence: 92%

Thrombospondin-1 supports blood pressure by limiting eNOS activation and endothelial-dependent vasorelaxation

Bauer

Yan

Miller

et al. 2010

Cardiovascular Research

136

164

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Regional heterogeneity of substance P-induced endothelium-dependent contraction, relaxation, and -independent contraction in rabbit pulmonary arteries

Cited by 7 publications

References 21 publications

Brain regional angiogenic potential at the neurovascular unit during normal aging

Brain regional angiogenic potential at the neurovascular unit during normal aging

Long-term effects of prenatal hypoxia on endothelium-dependent relaxation responses in pulmonary arteries of adult sheep

Thrombospondin-1 supports blood pressure by limiting eNOS activation and endothelial-dependent vasorelaxation

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