Regional hematocrit ratio and interstitial fluid volume in the normal rat

Mejia, Robert

doi:10.1007/bf02136781

Cited by 4 publications

(8 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The blood volume of the lung amounted to about one-half of the lung weight in the 24-hr-old piglets and decreased with age to values observed in adult rats (23,33). Inasmuch as pulmonary blood volume and flow in air-ventilated lambs are closely correlated (41) and pulmonary blood flow increases with growth (13), we expected an increment rather than a downward trend in relative pulmonary blood volume of the piglets with age.…”

Section: Age (Days)mentioning

confidence: 90%

“…There are some appreciable deviations of the piglets' organ blood volumes from those measured in adult animals. The blood volumes in the liver, skeleton, and gastrointestinal tract diminished markedly in the growing piglets, but the values of these organs measured in the 2-wk-old piglets were still above those noted in adult animals (2,5,9,23,30,33). The decrease in blood volume of the liver and the skeleton coincides with reduction of erythropoiesis in these organs resulting from iron deficiency (1 1).…”

Section: Age (Days)mentioning

confidence: 99%

“…cats (40), rabbits (2, 40), and hamsters (38). Special attention has been given to the renal hematocrit, which is about one-half that of the central circulation (8,9,12,23,28,30,33,35,38,40). The origin of the "extra plasma" is obscure.…”

Section: Age (Days)mentioning

confidence: 99%

“…measurement of total red cell mass and plasma volume, activities of 0.5 ml of blood and of the standards were counted in a welltype scintillation counter. Total body and organ hematocrits were Regional distribution of blood volume has been extensively calculated from the ratio of the corresponding red cell mass and studied in adult animals (1, 2,5,8,9,12,23,30,33,40). Little is blood volume values.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Blood Volume and Hematocrit in Various Organs in Newborn Piglets

Linderkamp¹,

Berg²,

Betke³

et al. 1980

Pediatr Res

View full text Add to dashboard Cite

Summaryborn to healthy sows at known full term. All piglets were suckledPlasma volume and red cell mass of various organs in piglets aged 24 hr (n = 7) and 7 (n = 6). and 14 (n = 6) days were measured using eemTc-labeled albumin and "Cr-labeled red blood cells. Organ activities were counted in a whole-body counter. Blood volume and hematwrit were calculated. The blood volumes in $/g varied markedly between various organs. The lowest blood volumes at 24 hr of age were found in skin (21.9 + 5.0 pl/g), brain (33.3 ~t 8.4), and skeletal muscle (35.5 + 7.4). The highest values at this age were noted in liver (670.0 + 89.1), lung (533.8 f 80.7), spleen (332.0 + 82.8), and kidney (300.6 + 55.5). Blood volumes of about 150 pl/g were observed in heart muscle and thyroid gland and those of about 100 pl/g in thymus and gastrointestinal tract.The total blood volume was 100.2 * 3.9 pl/g at 24 hr and remained unchanged during the first 2 wk of life. A significant decrease in relative blood volume with growth was noted in liver and lung ( P c 0.01), and in skeleton ( P < 0.05). The blood volume, contained in the great vessels outside the organs, increased from 29.5 * 5.5% of total blood volume at 24 hr to 31.2 * 5.7% at 7 days and to 38.2 k 7.5% at 14 days of life. The total body/venous hematwrit ratio was about 0.84. Accordingly, tissue hematwrits of most organs were below the venous hematocrit. Only in spleen was the tissue/ venous hematwrit ratio (TH/VH) higher than 1.0. TH/VH of brain, gastrointestinal tract, thyroid gland, and thymus approached unity. The lowest TH/VH was found in kidney (0.54 f 0.08 at day 1). In skin, the TH/VH decreased from 0.98 * 0.10 to 0.82 * 0.07 during the first 2 wk of life.by their mothers. They were not treated 4 t i iron. The right atrium was cannulated with polyethylen catheters via the right external jugular vein for injection and withdrawal of blood. Each piglet received local analgesia with procaine at the site of catheterization, but no systemic drugs. Two ml of blood were withdrawn from the catheter and centrifu ed for 5 min at 1000 x g. The red R blood cells were labeled with chromium (17). Salt-poor human serum albumin (25%) was labeled by means of a commercial kit for electrolytic preparation of -technetium (New England Nuclear Corp., North Billerica, MA). Each preparation of *"Tclabeled albumin was carefully checked for particulate matter, turbidity, and pH (6). Labeling efficiency was determined with trichloracetic acid and membrane filtration (14). Batches containing more than 1% of unbound -Tc were discarded. One postmix blood sample of 1 ml was taken 10 min after injection of labeled red blood cells and 5 min after injection of labeled albumin. Previous equilibration studies have shown that mixing of red blood cells and of albumin are complete within that period (16). Loss of albumin from the intravascular space has been found negligible in this short time. The piglets were killed immediately after the labeling and blood collecting procedure by injection of 2 mval KC1 via the ca...

show abstract

Section: Age (Days)mentioning

confidence: 90%

Section: Age (Days)mentioning

confidence: 99%

Section: Age (Days)mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Blood Volume and Hematocrit in Various Organs in Newborn Piglets

Linderkamp¹,

Berg²,

Betke³

et al. 1980

Pediatr Res

View full text Add to dashboard Cite

show abstract

“…[23][24][25] Estimated in vivo OPA titers of 45, 62 and 32 conferred protection against bacteremia from the serotype 1, 3 and 5 challenge doses, respectively, in the neonatal rats passively immunized with pooled post-immunization era at a 1:5 dilution from the macaques immunized with PCV13. Table 5 shows the measured serotype 3 OPA titers of the PCV7 and PCV13 human serum pools, the estimated serotype 3 OPA titers after 1:3 and 1:5 dilutions, as administered to the neonatal rats and the estimated final in vivo serotype 3 OPA titers of the PCV7 and PCV13 serum pools, based on the additional dilution of the serum pools into the approximately 0.25 mL plasma volume of the neonatal rats.…”

confidence: 99%

13-valent pneumococcal conjugate vaccine immune sera protects against pneumococcal serotype 1, 3, and 5 bacteremia in a neonatal rat challenge model

Fernsten

Mason²,

Yu³

et al. 2011

Human Vaccines

View full text Add to dashboard Cite

show abstract

Evidence for a hepatic‐renal antagonism in the production of hepatic erythropoietin in a phenylhydrazine‐induced compensated hemolytic state in the rat

Dornfest

Naughton

Lapin

et al. 1985

Journal of Surgical Oncology

View full text Add to dashboard Cite

A compensated hemolytic state was induced in rats by injection of phenylhydrazine (PHZ) over a 6-week period. The liver and kidney were perfused to determine the levels and time of appearance of a hepatic erythropoietic factor (HEF) which induces the production of hepatic erythropoietin (Ep) and its antagonist, a renal inhibitory factor (RIF). Erythropoietin assays on the perfusates have been previously reported. The amount of HEF in perfusates recovered from the livers of the PHZ-treated rats was significantly higher during the 4th to 5th weeks of treatment, coinciding with the time of increased liver production of Ep. During the 6th week of PHZ treatment, the titer of RIF in perfusates recovered from the kidney was markedly increased and the HEF titer was decreased to near the control level, suggesting inhibitory action of RIF on HEF synthesis and/or effect. These findings indicate that an HEF/RIF control mechanism regulates hepatic Ep production in this compensated hemolytic condition.

show abstract

Regional hematocrit ratio and interstitial fluid volume in the normal rat

Cited by 4 publications

References 5 publications

Blood Volume and Hematocrit in Various Organs in Newborn Piglets

Blood Volume and Hematocrit in Various Organs in Newborn Piglets

13-valent pneumococcal conjugate vaccine immune sera protects against pneumococcal serotype 1, 3, and 5 bacteremia in a neonatal rat challenge model

Evidence for a hepatic‐renal antagonism in the production of hepatic erythropoietin in a phenylhydrazine‐induced compensated hemolytic state in the rat

Contact Info

Product

Resources

About