Regional Expression Patterns of T1r Family in the Mouse Tongue

Kusakabe, Yuko; Kim, Mi-Ryung; Miura, Hirohito; Shindo, Yoichiro; Ninomiya, Yuzo; Hino, Akihiro

doi:10.1093/chemse/bjh094

Cited by 11 publications

(11 citation statements)

References 8 publications

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“…In the taste buds of the posterior part of the tongue, taste receptor cells that express Tas1R3 and Tas1R2 seldom express of α-gustducin [56]. The co-expression of Tas1R3 and gustducin does, however occur in taste buds of fungiform papillae and palate [57,58]. …”

Section: Discussionmentioning

confidence: 99%

Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

et al. 2011

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BackgroundChemical irritation of airway mucosa elicits a variety of reflex responses such as coughing, apnea, and laryngeal closure. Inhaled irritants can activate either chemosensitive free nerve endings, laryngeal taste buds or solitary chemosensory cells (SCCs). The SCC population lies in the nasal respiratory epithelium, vomeronasal organ, and larynx, as well as deeper in the airway. The objective of this study is to map the distribution of SCCs within the airways and to determine the elements of the chemosensory transduction cascade expressed in these SCCs.MethodsWe utilized a combination of immunohistochemistry and molecular techniques (rtPCR and in situ hybridization) on rats and transgenic mice where the Tas1R3 or TRPM5 promoter drives expression of green fluorescent protein (GFP).ResultsEpithelial SCCs specialized for chemoreception are distributed throughout much of the respiratory tree of rodents. These cells express elements of the taste transduction cascade, including Tas1R and Tas2R receptor molecules, α-gustducin, PLCβ2 and TrpM5. The Tas2R bitter taste receptors are present throughout the entire respiratory tract. In contrast, the Tas1R sweet/umami taste receptors are expressed by numerous SCCs in the nasal cavity, but decrease in prevalence in the trachea, and are absent in the lower airways.ConclusionsElements of the taste transduction cascade including taste receptors are expressed by SCCs distributed throughout the airways. In the nasal cavity, SCCs, expressing Tas1R and Tas2R taste receptors, mediate detection of irritants and foreign substances which trigger trigeminally-mediated protective airway reflexes. Lower in the respiratory tract, similar chemosensory cells are not related to the trigeminal nerve but may still trigger local epithelial responses to irritants. In total, SCCs should be considered chemoreceptor cells that help in preventing damage to the respiratory tract caused by inhaled irritants and pathogens.

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Section: Discussionmentioning

confidence: 99%

Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

et al. 2011

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“…Although T1R1, T1R2, and T1R3 can be found in taste buds of the anterior tongue, posterior tongue and palate, the relative expression of each T1R subunit varies across taste receptor fields [37, 45–46, 51, 60–61, 83]. In an initial study of expression patterns of T1R1 and T1R2 that combined mouse and rat tissues, T1R1 was found to be expressed in taste bud cells from fungiform papillae, which are found in the anterior two-thirds of the tongue and palate, and expressed to a lesser extent in the taste bud cells of the circumvallate and foliate papillae in the posterior tongue.…”

Section: Localization Of T1r Expressionmentioning

confidence: 99%

The functional role of the T1R family of receptors in sweet taste and feeding

Treesukosol

Smith

Spector

2011

Physiology & Behavior

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The discovery of the T1R family of Class C G protein-coupled receptors in the peripheral gustatory system a decade ago has been a tremendous advance for taste research, and its conceptual reach has extended to other organ systems. There are three proteins in the family, T1R1, T1R2, and T1R3, encoded by their respective genes, Tas1r1, Tas1r2, and Tas1r3. T1R2 combines with T1R3 to form a heterodimer that binds with sugars and other sweeteners. T1R3 also combines with T1R1 to form a heterodimer that binds with L-amino acids. These proteins are expressed not only in taste bud cells, but one or more of these T1Rs have also been identified in the nasal epithelium, gut, pancreas, liver, kidney, testes and brain in various mammalian species. Here we review current perspectives regarding the functional role of these receptors, concentrating on sweet taste and feeding. We also discuss behavioral findings suggesting that a glucose polymer mixture, Polycose, which rodents avidly prefer, appears to activate a receptor that does not depend on the combined expression of T1R2 and T1R3. In addition, although the T1Rs have been implicated as playing a role in glucose sensing, T1R2 knock-out (KO) and T1R3 KO mice display normal chow and fluid intake as well as normal body weight compared with same-sex littermate wild type (WT) controls. Moreover, regardless of whether they are fasted or not, these KO mice do not differ from their WT counterparts in their Polycose intake across a broad range of concentrations in 30-min intake tests. The functional implications of these results and those in the literature are considered.

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“…One explanation for the differences is the demonstrated variations in the regional expression of taste receptors (e.g. T1Rs, T2Rs, ion channels such as ENaC and ASIC) among the taste regions of the oral cavity and between species [9,[53][54][55][56][57]. Further, there are important polymorphisms in the T1R and T2R proteins between species and among strains that contribute to the differences in taste sensitivities [54,58,59].…”

Section: What Neurotransmitters Do Taste Cells Release?mentioning

confidence: 99%

Signaling in the Chemosensory Systems

Roper

2006

Cell. Mol. Life Sci.

106

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Taste bud cells communicate with sensory afferent fibers and may also exchange information with adjacent cells. Indeed, communication between taste cells via conventional and/or novel synaptic interactions may occur prior to signal output to primary afferent fibers. This review discusses synaptic processing in taste buds and summarizes results showing that it is now possible to measure real-time release of synaptic transmitters during taste stimulation using cellular biosensors. There is strong evidence that serotonin and ATP play a role in cell-to-cell signaling and sensory output in the gustatory end organs.

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Regional Expression Patterns of T1r Family in the Mouse Tongue

Cited by 11 publications

References 8 publications

Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

The functional role of the T1R family of receptors in sweet taste and feeding

Signaling in the Chemosensory Systems

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