Regional expression of a gene encoding a neuron-specific Na+-dependent inorganic phosphate cotransporter (DNPI) in the rat forebrain

Hisano, Setsuji; Hoshi, Koichi; Ikeda, Yayoi; Maruyama, Daisuke; Kanemoto, Mizuki; Ichijo, Hiroyuki; Kojima, Itaru; Takeda, Jun; Nogami, Hisashi

doi:10.1016/s0169-328x(00)00194-7

Cited by 780 publications

(152 citation statements)

References 21 publications

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“…Each probe exhibited a labeling pattern distinct from the other two and in good accord with previous isotopic (Hisano et al, 2000;Fremeau et al, 2001;Herzog et al, 2001Herzog et al, , 2004 and nonisotopic (Hur and Zaborszky, 2005) in situ hybridization studies. Hybridization conditions first were adjusted to eliminate background or weak "Nissling" of structures known to lack VGLUT mRNA (Hisano et al, 2000;Fremeau et al, 2001;Herzog et al, 2001Herzog et al, , 2004Hur and Zaborszky, 2005). Increasing the concentration of VGLUT3 cRNA produced weak labeling in structures positive for VGLUT2 mRNA, such as the laterodorsal tegmental nucleus, various thalamic nuclei, and the superior colliculus.…”

Section: Methodological Considerationssupporting

confidence: 88%

Glutamatergic Afferents of the Ventral Tegmental Area in the Rat

Geisler¹,

Derst²,

Veh³

et al. 2007

J. Neurosci.

419

425

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Glutamatergic inputs to the ventral tegmental area (VTA), thought crucial to the capacity of the VTA to detect and signal stimulus salience, have been reported to arise in but a few structures. However, the afferent system of the VTA comprises very abundant neurons within a large formation extending from the prefrontal cortex to the caudal brainstem. Neurons in nearly all parts of this continuum may be glutamatergic and equivalently important to VTA function. Thus, we sought to identify the full range of glutamatergic inputs to the VTA by combining retrograde transport of wheat germ agglutinin-bound gold after injections into the VTA with nonisotopic in situ hybridization of the vesicular glutamate transporters (VGLUTs) 1, 2, and 3. We found glutamatergic neurons innervating the VTA in almost all structures projecting there and that a majority of these are subcortical and VGLUT2 mRNA positive. The tremendous convergence of glutamatergic afferents from many brain areas in the VTA suggests that (1) the function of the VTA requires integration of manifold and diverse bits of information and (2) the activity of the VTA reflects the ongoing activities of various combinations of its afferents.

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Section: Methodological Considerationssupporting

confidence: 88%

Glutamatergic Afferents of the Ventral Tegmental Area in the Rat

Geisler¹,

Derst²,

Veh³

et al. 2007

J. Neurosci.

419

425

View full text Add to dashboard Cite

show abstract

“…6 I, J ) (supplemental figure, available at www.jneurosci.org as supplemental material) throughout development. Similar regional differences in VGLUT2 mRNA expression in neocortical layers have been reported in the adult rat (Hisano et al, 2000).…”

Section: Coexpression and Endogenous Regulation Of Vglut1 And Vglut2 supporting

confidence: 81%

“…Indeed, single-cell RT-PCR of cytoplasm from individual layer IV excitatory neuronal cell bodies of adult rat cortex slices indicates the presence of VGLUT1-encoded pyramidalshaped neurons and significant subpopulations of VGLUT1/ VGLUT2 coexpressing and VGLUT1-only oval/round-shaped neurons, which may represent spiny layer IV excitatory neurons. These latter cells, which include spiny stellate and star pyramidal neurons (Cowan and Stricker, 2004;Staiger et al, 2004), receive predominantly VGLUT2-encoded thalamic excitatory input (Hisano et al, 2000;Fujiyama et al, 2001) and distribute this subcortical input to target cells in other cortical layers, in particular to those in layer II/III, but also to layer V/VI neurons (Feldmeyer et al, 1999), in which we also show the presence of VGLUT1/VGLUT2 coexpressing neurons. Interestingly, the star pyramidal neurons of layer IV in visual cortex have been implicated recently in driving activity-dependent changes in E/I balance that results from monocular deprivation during development (Maffei et al, 2004).…”

Section: Coexpression Of Vglut1 and Vglut2 In Discrete Populations Ofsupporting

confidence: 53%

Homeostatic Scaling of Vesicular Glutamate and GABA Transporter Expression in Rat Neocortical Circuits

Gois

Schäfer²,

Defamie³

et al. 2005

J. Neurosci.

172

183

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Homeostatic control of pyramidal neuron firing rate involves a functional balance of feedforward excitation and feedback inhibition in neocortical circuits. Here, we reveal a dynamic scaling in vesicular excitatory (vesicular glutamate transporters VGLUT1 and VGLUT2) and inhibitory (vesicular inhibitory amino acid transporter VIAAT) transporter mRNA and synaptic protein expression in rat neocortical neuronal cultures, using a well established in vitro protocol to induce homeostatic plasticity. During the second and third week of synaptic differentiation, the predominant vesicular transporters expressed in neocortical neurons, VGLUT1 and VIAAT, are both dramatically upregulated. In mature cultures, VGLUT1 and VIAAT exhibit bidirectional and opposite regulation by prolonged activity changes. Endogenous coregulation during development and homeostatic scaling of the expression of the transporters in functionally differentiated cultures may serve to control vesicular glutamate and GABA filling and adjust functional presynaptic excitatory/inhibitory balance. Unexpectedly, hyperexcitation in differentiated cultures triggers a striking increase in VGLUT2 mRNA and synaptic protein, whereas decreased excitation reduces levels. VGLUT2 mRNA and protein are expressed in subsets of VGLUT1-encoded neocortical neurons that we identify in primary cultures and in neocortex in situ and in vivo. After prolonged hyperexcitation, downregulation of VGLUT1/ synaptophysin intensity ratios at most synapses is observed, whereas a subset of VGLUT1-containing boutons selectively increase the expression of VGLUT2. Bidirectional and opposite regulation of VGLUT1 and VGLUT2 by activity may serve as positive or negative feedback regulators for cortical synaptic transmission. Intracortical VGLUT1/VGLUT2 coexpressing neurons have the capacity to independently modulate the level of expression of either transporter at discrete synapses and therefore may serve as a plastic interface between subcortical thalamic input (VGLUT2) and cortical output (VGLUT1) neurons.

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“…Most VGLUT3-immunoreactive neurons in the lateral hypothalamus appear to be magnocellular neurons and may partially coincide with the melanin concentrating hormone containing neurons known to display wide projections to many areas of the brain including limbic and cortical areas where they are proposed to participate with serotoninergic systems in the regulation of sleep and feeding behavior (21,67). Hypothalamic VGLUT3 neurons are also located in a region considered an "aggression area" in the rat (68).…”

Section: Vglut3 Expression Defines Novel Subsets Of Excitatorymentioning

confidence: 98%

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

Schäfer¹,

Varoqui²,

Defamie³

et al. 2002

Journal of Biological Chemistry

369

392

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We have cloned and functionally characterized a third isoform of a vesicular glutamate transporter (VGLUT3) expressed on synaptic vesicles that identifies a distinct glutamatergic system in the brain that is partly and selectively promiscuous with cholinergic and serotoninergic transmission. Transport activity was specific for glutamate, was H ؉ -dependent, was stimulated by Cl ؊ ion, and was inhibited by Rose Bengal and trypan blue. Northern analysis revealed higher mRNA levels in early postnatal development than in adult brain. Restricted patterns of mRNA expression were observed in presumed interneurons in cortex and hippocampus, and projection systems were observed in the lateral and ventrolateral hypothalamic nuclei, limbic system, and brainstem. Double in situ hybridization histochemistry for vesicular acetylcholine transporter identified VGLUT3 neurons in the striatum as cholinergic interneurons, whereas VGLUT3 mRNA and protein were absent from all other cholinergic cell groups. In the brainstem VGLUT3 mRNA was concentrated in mesopontine raphé nuclei. VGLUT3 immunoreactivity was present throughout the brain in a diffuse system of thick and thin beaded varicose fibers much less abundant than, and strictly separated from, VGLUT1 or VGLUT2 synapses. Co-existence of VGLUT3 in VMAT2-positive and tyrosine hydroxylase -negative varicosities only in the cerebral cortex and hippocampus and in subsets of tryptophan hydroxylase-positive cell bodies and processes in differentiating primary raphé neurons in vitro indicates selective and target-specific expression of the glutamatergic/serotoninergic synaptic phenotype.

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Regional expression of a gene encoding a neuron-specific Na+-dependent inorganic phosphate cotransporter (DNPI) in the rat forebrain

Cited by 780 publications

References 21 publications

Glutamatergic Afferents of the Ventral Tegmental Area in the Rat

Glutamatergic Afferents of the Ventral Tegmental Area in the Rat

Homeostatic Scaling of Vesicular Glutamate and GABA Transporter Expression in Rat Neocortical Circuits

Molecular Cloning and Functional Identification of Mouse Vesicular Glutamate Transporter 3 and Its Expression in Subsets of Novel Excitatory Neurons

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