Regional disturbances in blood flow and metabolism in equine limb wound healing with formation of exuberant granulation tissue

Sørensen, Mette Aamand; Petersen, Lone; Bundgaard, Louise; Toft, Nils; Jacobsen, Stine

doi:10.1111/wrr.12207

Cited by 27 publications

(29 citation statements)

References 39 publications

(90 reference statements)

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“…Nicotine uptake via gums, patches or inhalation causes nicotine serum concentrations to be similar with those of active smokers . Transdermal nicotine patches have been used to evaluate nicotine's effects on skin . In the current study, the higher plasma levels of cotinine, the most important and stable nicotine metabolite in mammalian species , in the nicotine‐treated mice indicated the systemic distribution of nicotine.…”

Section: Discussionmentioning

confidence: 71%

Nicotine affects cutaneous wound healing in stressed mice

Almeida

Romana‐Souza

Machado

et al. 2013

Experimental Dermatology

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Stress is an important condition of modern life. Nicotine addiction can modulate the physiological response to stress. Cutaneous healing is a complex process resulting in scar formation, which can be delayed by stress. Therefore, the aim of this study was to investigate the effects of nicotine administration on cutaneous wound healing in chronically stressed mice. Male mice were submitted to rotational stress, whereas control animals were not subjected to stress. These stressed and control animals were treated with a transdermal nicotine patch that was changed every day. A full-thickness excisional lesion was also generated, and 14 days later, lesions had recovered. However, the Stress + Nicotine group presented a delay in wound contraction. These wounds showed a decrease in inflammatory cell infiltration and lower expression of transforming growth factor-β (TGF-β), whereas there was an increase in angiogenesis and tumor necrosis factor-α (TNF-α) expression. In vitro fibroblast migration was also impaired by the nicotine treatment of stressed-stimulated cells. In conclusion, nicotine administration potentiates the delay in wound closure observed in mice submitted to stress.

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Section: Discussionmentioning

confidence: 71%

Nicotine affects cutaneous wound healing in stressed mice

Almeida

Romana‐Souza

Machado

et al. 2013

Experimental Dermatology

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“…Details concerning the included animals, protocol for creation of experimental wounds, sampling of blood, and collection of interstitial fluid by microdialysis are described in Sørensen et al, 2014 . In short, five healthy geldings each had a total of 12 full‐thickness wounds made; three wounds on each shoulder over the deltoid muscle penetrating the superficial part of the fascia and three on the lateral aspect of each metatarsus extending to the periosteum, in a vertical column, 2.5 × 2.5 cm, 3 cm apart.…”

Section: Methodsmentioning

confidence: 99%

A selected reaction monitoring‐based analysis of acute phase proteins in interstitial fluids from experimental equine wounds healing by secondary intention

Bundgaard

Bendixen

Sørensen

et al. 2016

Wound Repair Regeneration

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In horses, pathological healing with formation of exuberant granulation tissue (EGT) is a particular problem in limb wounds, whereas body wounds tend to heal without complications. Chronic inflammation has been proposed to be central to the pathogenesis of EGT. This study aimed to investigate levels of inflammatory acute phase proteins (APPs) in interstitial fluid from wounds in horses. A novel approach for absolute quantification of proteins, selected reaction monitoring (SRM)-based mass spectrometry in combination with a quantification concatamer (QconCAT), was used for the quantification of five established equine APPs (fibrinogen, serum amyloid A, ceruloplasmin, haptoglobin, and plasminogen) and three proposed equine APPs (prothrombin, α-2-macroglobulin, and α-1-antitrypsin). Wound interstitial fluid was recovered by large pore microdialysis from experimental body and limb wounds from five horses at days 1, 2, 7, and 14 after wounding and healing without (body) and with (limb) the formation of EGT. The QconCAT included proteotypic peptides representing each of the protein targets and was used to direct the design of a gene, which was expressed in Escherichia coli in a media supplemented with stable isotopes for metabolically labeling of standard peptides. Co-analysis of wound interstitial fluid samples with the stable isotope-labeled QconCAT tryptic peptides in known amounts enabled quantification of the APPs in absolute terms. The concentrations of fibrinogen, haptoglobin, ceruloplasmin, prothrombin, and α-1-antitrypsin in dialysate from limb wounds were significantly higher than in dialysate from body wounds. This is the first report of simultaneous analysis of a panel of APPs using the QconCAT-SRM technology. The microdialysis technique in combination with the QconCAT-SRM-based approach proved useful for quantification of the investigated proteins in the wound interstitial fluid, and the results indicated that there is a state of sustained inflammation in equine wounds healing with formation of EGT.

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“…Failure to achieve therapeutic levels of antimicrobials within wound beds could therefore result from vascular compromise. Blood supply to distal limb wounds may be compromised by infection, necrotic tissue, and microvascular occlusion . There is conflicting evidence on the effects of IVRLP on limb vasculature.…”

Section: Discussionmentioning

confidence: 99%