Regional distribution and species variation of prolactin binding sites in the brain

Muccioli, Giampiero; Bellussi, G; Ghè, Corrado; Pagnini, G; Carlo, R. Di

doi:10.1016/0016-6480(88)90031-7

Cited by 36 publications

(17 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to its classical effects on the mammary gland, PRL has been shown to have a number of other actions, all of which are initiated by an interaction with specific high-affinity receptors located on the plasma membrane and widely distrib uted in a number of tissues [26,27], From our data it appears evident that in the rat, as pre viously observed for other animals [6][7][8], specific binding sites for PRL are present in the hypothalamus and substantia nigra. The hormonal specificity and PRL affinity of these binding sites in the rat hypothalamus are very similar to those found in the rat liver and ovary as well as to those described by different authors in other PRL target tissues [27][28][29].…”

Section: Discussionsupporting

confidence: 51%

“…Conversely at tempts to identify PRL binding sites in rat hypothalamus by in vitro competitive binding assays have so far been unsuccessful [8,13,14], Moreover in the rat in vitro autoradiographic analy sis of whole hypothalamus [14] and in vivo autoradiographic analysis of the median eminence [15,16] have generally failed to Received: December 18, 1989 Accepted after revision: June 18, 1990 demonstrate significant specific binding for PRL. Only very re cently, Barton et al [ 17] were able to demonstrate, using in vitro autoradiography with l25I-ovine PRL the presence of specific binding sites for PRL in the median eminence of the female rat brain.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Distribution and Characterization of Prolactin Binding Sites in the Male and Female Rat Brain: Effects of Hypophysectomy and Ovariectomy

1991

Self Cite

View full text Add to dashboard Cite

The binding of ¹²⁵I-labeled rat prolactin (¹²⁵I-rat PRL) to membranes from different regions of the rat brain was studied. Among these regions the hypothalamus showed the highest specific binding. Clearly detectable specific binding was also observed in substantia nigra, whereas it was very scanty in other brain regions. No significant sex differences in PRL binding to various brain regions were observed, except for hypothalamus where a higher binding was observed in female rats. The binding of ¹²⁵I-rat PRL to hypothalamus from female rats was inhibited in a dose-dependent manner by both unlabeled rat and ovine PRL but not by several other polypeptide hormones. Scatchard analysis of the binding revealed the presence of the binding sites with low capacity and high affinity for rat ligand. Ovariectomy markedly decreased PRL binding in the hypothalamus; an even more pronounced decrease was found after hypophysectomy of female animals. A treatment with estradiol restored the PRL binding in the ovariectomized rats to above normal levels. These results of in vitro biochemical analysis together with the experimental modulation of hormonal status provide strong preliminary evidence for the presence of PRL binding sites in rat brain.

show abstract

Section: Discussionsupporting

confidence: 51%

mentioning

confidence: 99%

Distribution and Characterization of Prolactin Binding Sites in the Male and Female Rat Brain: Effects of Hypophysectomy and Ovariectomy

1991

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although the sites of PRL action in promoting these behavioural changes remain largely unexplored, neurophysiological and neurochemical evidence suggests that PRL is capable of influencing brain activity directly (14)(15)(16). This view is strengthened by evidence for PRL binding sites in specific forebrain regions of the rabbit (17), pig (18), horse (19), toad (20) and ring dove (21,22). PRL binding has also been reported in the choroid plexus of rat (23), horse, calf (19), ring dove (24), sheep, pig and rabbit (18).…”

mentioning

confidence: 69%

“…This view is strengthened by evidence for PRL binding sites in specific forebrain regions of the rabbit (17), pig (18), horse (19), toad (20) and ring dove (21,22). PRL binding has also been reported in the choroid plexus of rat (23), horse, calf (19), ring dove (24), sheep, pig and rabbit (18). Although their function has yet to be established, it has been proposed that PRL binding sites in the choroid plexus may be involved in the transport of PRL from blood to cerebrospinal fluid (25), thereby providing a possible route by which blood-borne PRL could gain access to target cells in periventricular brain areas.…”

mentioning

confidence: 93%

Changes in Feeding Activity, Plasma Luteinizing Hormone, and Testes Weight in Ring Doves Following Hypothalamic Injections of Prolactin

Foreman

Lea

Buntin

1990

J Neuroendocrinology

View full text Add to dashboard Cite

Microinjections of ovine prolactin were administered unilaterally to the ventromedial hypothalamic nucleus and the preopticsuprachiasmatic region in adult male ring doves in an attempt to determine the site(s) at which intracranial injections of prolactin act to alter feeding behaviour and gonadotropin secretion in this species. Food intake and body weight were measured daily during a 6-day pretreatment period and the 5-day treatment period that immediately followed. During the treatment period, birds received twice daily injections (0.5 PI) of either 2.5 ng ovine prolactin or saline vehicle. An additional group of birds with cannulae in the ventromedial nucleus were given twice daily injections of 25 ng ovine prolactin. Although food consumption was unaffected by low dose prolactin treatment, birds given 25 ng prolactin injections into the ventromedial nucleus showed a significant augmentation in food intake. Injections of 25 ng prolactin into the preoptic area also increased feeding; however, the magnitude of this hyperphagic response, as expressed relative to pretreatment levels, was less than that observed following prolactin injection into the ventromedial nucleus. No differences were observed between prolactin-treated and vehicle-treated birds in either cannulation group when testes weights and plasma luteinizing hormone concentrations were compared at the end of the treatment period. However, the possibility that prolactin influenced changes in luteinizing hormone and testes weight relative to baseline values could not be assessed due to constraints imposed by the experimental paradigm used. These results suggest that prolactin-sensitive neurons in the ventromedial hypothalamic region and the preoptic area are potential sites of prolactin action in promoting hyperphagia in ring doves. However, the role of these sites in mediating prolactin-induced suppression of gonadotropin secretion in this species remains to be clarified.

show abstract

“…The LF is the only PRLR isoform that has been identified in pigs to date. As in other species, the pPRLR is expressed in various tissues including the mammary gland from nulliparous (Trott et al 2007), pregnant (Plaut et al 1989, Young et al 1990, and lactating females (Sakai et al 1985, Berthon et al 1987, liver, kidney (Trott et al 2007), ovary (Bramley & Menzies 1987, Slomczynska et al 2001, uterus (Young & Bazer 1989), adrenal cortex (Klemcke et al 1989), and brain (Muccioli et al 1988). However, the factors that regulate pPRLR expression have not been assessed.…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific regulation of porcine prolactin receptor expression by estrogen, progesterone, and prolactin

Trott

Horigan

Gloviczki

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

Prolactin (PRL) acts through its receptor (PRLR) via both endocrine and local paracrine/autocrine pathways to regulate biological processes including reproduction and lactation. We analyzed the tissue-and stage of gestationspecific regulation of PRL and PRLR expression in various tissues of pigs. Abundance of pPRLR-long form (LF) mRNA increased in the mammary gland and endometrium during gestation while in other tissues it remained constant. There was a parallel increase in the abundance of the pPRLR-LF protein in the mammary gland and endometrium during gestation. We determined the hormonal regulation of pPRLR-LF mRNA expression in various tissues from ovariectomized, hypoprolactinemic gilts given combinations of the replacement hormones estrogen (E 2 ), progestin (P), and/or haloperidol-induced PRL. Abundance of pPRLR-LF mRNA in kidney and liver was unaffected by hormone treatments. Expression of uterine pPRLR-LF mRNA was induced by E 2 whereas the effect of E 2 was abolished by co-administering P. The expression of pPRLR-LF mRNA in the mammary gland stroma was induced by PRL, whereas E 2 induced its expression in the epithelium. In contrast to these changes in pPRLR expression, pPRL expression was relatively constant and low during gestation in all tissues except the pituitary. Taken together, these data reveal that specific combinations of E 2 , P, and PRL differentially regulate pPRLR-LF expression in the endometrium and mammary glands, and that the action of PRL on its target tissues is dependent upon pPRLR-LF abundance more so than the local PRL expression.

show abstract

Regional distribution and species variation of prolactin binding sites in the brain

Cited by 36 publications

References 29 publications

Distribution and Characterization of Prolactin Binding Sites in the Male and Female Rat Brain: Effects of Hypophysectomy and Ovariectomy

Distribution and Characterization of Prolactin Binding Sites in the Male and Female Rat Brain: Effects of Hypophysectomy and Ovariectomy

Changes in Feeding Activity, Plasma Luteinizing Hormone, and Testes Weight in Ring Doves Following Hypothalamic Injections of Prolactin

Tissue-specific regulation of porcine prolactin receptor expression by estrogen, progesterone, and prolactin

Contact Info

Product

Resources

About