Regional differences in the placental levels of oxidative stress markers in pre‐eclampsia

Sahay, Akriti S; Sundrani, Deepali; Wagh, Girija; Mehendale, Savita; Joshi, Sadhana

doi:10.1016/j.ijgo.2015.03.001

Cited by 38 publications

(30 citation statements)

References 24 publications

(32 reference statements)

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“…In our earlier study, we have reported higher oxidative stress indicated by the increased MDA levels in all regions of preterm preeclampsia group as compared to the normotensive control group [24]. Studies from our department and by others suggest that there is a presence of higher oxidative stress in women with preeclampsia and in preeclampsia placenta [29,30].…”

Section: Discussionmentioning

confidence: 49%

“…In addition, we have also reported increased oxidative stress in the central maternal region as compared to central fetal region in the women with preeclampsia delivering preterm [24].…”

Section: Introductionmentioning

confidence: 58%

“…Amongst the 47 women with preeclampsia, 21 women delivered at term (!37 weeks) and 26 delivered preterm (<37 weeks). The power of the current study was calculated based on our previous study which examined region specific changes in the levels of placental malondialdehyde (MDA) levels (p < 0.05) between normotensive women (n ¼ 35) and women with preeclampsia delivering both at term and preterm (n ¼ 25) [24]. For the current study the sample size was 50 normotensive control women, 21 women with preeclampsia delivering at term and 26 women with preeclampsia delivering preterm were included.…”

Section: Subjectsmentioning

confidence: 99%

“…From the chorionic plate (representing fetal side) the membranes were removed thus the fetal samples did not include the membranes, whereas the maternal samples from the basal plate (representing maternal side) included the decidua. Detailed method of sample collection has been described in our earlier study [24]. Briefly, placental tissue samples were collected from four different regions in the placenta: i.e i.…”

Section: Sample Collection and Processingmentioning

confidence: 99%

“…Placental tissues were homogenized in chilled PBS as described in our earlier study [24]. Total protein content of the lysate was then estimated by Lowry method [26].…”

Section: Preparation Of Placental Tissue Homogenatesmentioning

confidence: 99%

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Neurotrophin levels in different regions of the placenta and their association with birth outcome and blood pressure

et al. 2015

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Section: Discussionmentioning

confidence: 49%

“…In addition, we have also reported increased oxidative stress in the central maternal region as compared to central fetal region in the women with preeclampsia delivering preterm [24].…”

Section: Introductionmentioning

confidence: 58%

Section: Subjectsmentioning

confidence: 99%

Section: Sample Collection and Processingmentioning

confidence: 99%

“…Placental tissues were homogenized in chilled PBS as described in our earlier study [24]. Total protein content of the lysate was then estimated by Lowry method [26].…”

Section: Preparation Of Placental Tissue Homogenatesmentioning

confidence: 99%

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Neurotrophin levels in different regions of the placenta and their association with birth outcome and blood pressure

et al. 2015

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Regional changes of placental vascularization in preeclampsia: A review

2015

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Preeclampsia is characterized by vascular dysfunction and results in maternal and fetal morbidity and mortality. The placenta plays a critical role in the growth and development of the fetus, and recent studies indicate that placental architecture, oxygen availability, and oxidative stress indices vary across different regions of the placenta. Our earlier studies have reported altered maternal angiogenesis and differential placental gene expression and methylation patterns of angiogenic factors in women with preeclampsia when compared with normotensive women. We have also demonstrated lower maternal and placental neurotrophin (NT) levels in women with preeclampsia. Studies suggest that oxidative stress is associated with proteases like matrix metalloproteinases (MMPs) and growth factors like NTs and angiogenic factors known to be involved in the process of angiogenesis. Recently, we have reported regionwise differential oxidative stress, antioxidant enzyme activity, and NT levels in placenta from normotensive control women and women with preeclampsia. The current review describes the regional changes in the placenta and highlights the role of placental oxidative stress in influencing regional differences in the expression of angiogenic factors, MMPs, and NTs. This review discusses the need for further research on various growth factors and proteins involved in the process of placental development across different regions of the placenta. This would help to understand whether regional differences in these factors affect the growth and development of the fetus. V C 2015 IUBMB Life, 67(8): [619][620][621][622][623][624][625] 2015

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Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases ‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia placentae

Sahay

Jadhav

Sundrani

et al. 2018

J of Cellular Biochemistry

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Matrix metalloproteinases (MMPs) are involved in the extracellular matrix (ECM) remodeling during human placentation and parturition and have been shown to be associated with oxidative stress. Placental regional changes in oxygen availability and oxidative stress indices may influence regional differences in expression of MMPs. This study examines the protein and mRNA levels of MMP-2 and MMP-9 in different regions of the placenta in normotensive control (NC) women and women with preeclampsia (PE). Fifty-two NC women and 43 women with PE (18 delivered at term [T-PE] and 25 delivered preterm [PT-PE]) were recruited. Placental samples were taken from four regions: central basal (CM), central chorionic (CF), peripheral basal (PM), and peripheral chorionic (PF). MMP protein and mRNA levels were measured by ELISA and quantitative real time PCR, respectively. MMP-2 protein levels were higher in all the placental regions (P < 0.05) from PT-PE group as compared to the respective regions from the NC and T-PE groups. MMP-9 mRNA levels were higher in CM region as compared to CF and PM regions (P < 0.05) in the NC group and compared to CF and PF regions (P < 0.05) in the T-PE group. The MMP-9 mRNA levels were lower in the CF region in the PT-PE and T-PE groups (P < 0.05) as compared to the NC group. Elevated levels of MMP-2 protein levels were observed in all regions of PT-PE placenta possibly influencing the degradation of placental ECM. Lower mRNA expression of MMP-9 both in PT-PE and T-PE may contribute to a disturbed placental vascularization.

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Regional differences in the placental levels of oxidative stress markers in pre‐eclampsia

Abstract: Pre-eclampsia might be characterized by differential placental oxidative stress and antioxidant enzyme activity.

Cited by 38 publications

References 24 publications

Neurotrophin levels in different regions of the placenta and their association with birth outcome and blood pressure

Neurotrophin levels in different regions of the placenta and their association with birth outcome and blood pressure

Regional changes of placental vascularization in preeclampsia: A review

Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases ‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia placentae

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