Regional differences in sympathetic purinergic transmission along the length of the mouse vas deferens

Knight, David; D'Arbe, M.; Liang, Simon; Phillips, William D.; Lavidis, Nickolas A.

doi:10.1002/syn.10119

Cited by 16 publications

(13 citation statements)

References 39 publications

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“…There is regional variation, with dominance of purinergic signalling at the prostatic segment of the vas deferens, while noradrenergic signalling was more prominent in the epididymal segment ( Fig. 3a and b) [97, 211,345].…”

Section: Vas Deferensmentioning

confidence: 99%

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

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There are multiple roles for purinergic signalling in both male and female reproductive organs. ATP, released as a cotransmitter with noradrenaline from sympathetic nerves, contracts smooth muscle via P2X1 receptors in vas deferens, seminal vesicles, prostate and uterus, as well as in blood vessels. Male infertility occurs in P2X1 receptor knockout mice. Both short-and long-term trophic purinergic signalling occurs in reproductive organs. Purinergic signalling is involved in hormone secretion, penile erection, sperm motility and capacitation, and mucous production. Changes in purinoceptor expression occur in pathophysiological conditions, including pre-eclampsia, cancer and pain.

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Section: Vas Deferensmentioning

confidence: 99%

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

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“…Bars 50 μm mal and the prostatic portion. A faster contraction is evoked in the prostatic portion to exert a sphincter-like action as a mechanism for ejaculation (Knight et al 2003).…”

Section: Genital Systemmentioning

confidence: 99%

Differential expression of caveolin-3 in mouse smooth muscle cells in vivo

et al. 2006

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Expression of caveolin-1 and -3 in mouse smooth muscle cells in vivo was examined by immunohistochemistry. Caveolin-1 was detected in almost all smooth muscles examined, except for the pupillary dilator muscle, whereas caveolin-3 was present only in smooth muscles of some specific tissues. In the eye, the pupillary sphincter muscle was intensely positive for caveolin-3, whereas the ciliary muscle and pupillary dilator muscle were negative. In the gastrointestinal tract, caveolin-3 was detected in the inner circular layer, but not in the outer longitudinal layer. Vascular smooth muscle cells of the resistance-sized artery in the uterus and corpus cavernosum were intensely positive for caveolin-3, whereas those of the aorta were only weakly positive and those of the vena cava were negative. Caveolin-3 was also detected in smooth muscle cells of the urinary bladder, ureter, prostatic vas deferens, and seminal vesicle. The different levels of caveolin-3 expression among various smooth muscle tissues were confirmed by Western blot analysis. Even within the same muscle, the relative expression levels of caveolin-1 and -3 were variable among neighboring cells, suggesting distinct fine regulation of expression of these two caveolins. Moreover, even in the same cell, caveolin-1 and -3 showed different distributions. These results indicate that the two caveolins form distinct caveolae in smooth muscles, and that caveolin-1 and -3 serve different functions. Their differential expression may therefore be related to the functional diversity of smooth muscles.

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“…While variations in the post-synaptic specializations have been shown to affect synaptic strength in other terminals (e.g., Casanovas et al, 1999;Levenes et al, 2001;Lim et al, 1999), in the vas deferens only a small proportion of the P2X 1 -purinocpetors, which are evenly distributed over the entire surface of the smooth muscle (Knight et al, 2003;Lee et al, 2000;Liang et al, 2001;Vulchanova et al, 1996), are responsible for generating the spontaneous excitatory junction currents (Liang et al, 2001). This suggests that the variation in the strength of synaptic transmission in this preparation is pre-junctional in origin.…”

Section: Introductionmentioning

confidence: 99%

Correlation of non-uniform protein expression with variation in transmitter release probability

Knight¹,

Mann²,

Jackson³

et al. 2004

Synapse

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The strength of synaptic transmission is highly variable between different synapses. The present study examined some factors that may contribute to this variation in the strength of neurotransmission in sympathetic varicosities of the mouse vas deferens. Transmitter release was measured using a focal macropatch electrode placed over pairs of visualised varicosities. By regulating the calcium concentration of the solutions inside the recording electrode and in the bath independently of each other, transmitter release was restricted to one or two surface varicosities at each recording site. Using this technique, transmitter release probability was shown to be highly variable, even between adjacent varicosities on single axon branches. Very little variation was observed in the calcium influx following single impulse nerve stimulation between adjacent Oregon Green BAPTA-1 loaded varicosities. However, the staining intensities of three vesicular proteins, SV2, synaptophysin, and synaptotagmin 1, showed considerable variation between adjacent varicosities on single axon branches. This variation in staining intensity may be partly explained by variation in the density of synaptic vesicles. However, double staining experiments using two vesicular antigens showed some varicosities staining for one vesicular antigen, but not for the second, suggesting that the expression of these release machinery proteins is regulated locally within the varicosities. The results of the present study strengthen suggestions that synaptic strength is at least in part, regulated by variation in the expression of vesicular proteins.

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Regional differences in sympathetic purinergic transmission along the length of the mouse vas deferens

Cited by 16 publications

References 39 publications

Purinergic signalling in the reproductive system in health and disease

Purinergic signalling in the reproductive system in health and disease

Differential expression of caveolin-3 in mouse smooth muscle cells in vivo

Correlation of non-uniform protein expression with variation in transmitter release probability

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