Regional differences in expression of progesterone receptor in oviduct and uterus of rabbit during early pregnancy

Anzaldúa, Santiago René; Camacho‐Arroyo, Ignacio; Reyna-Neyra, Andrea; Pérez-Martínez, Mario; Cerbón, Marco

doi:10.1016/j.cbpa.2006.06.031

Cited by 18 publications

(8 citation statements)

References 27 publications

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“…As oestrogen and progesterone receptors along the rabbit oviduct vary according to the gonadal hormones concentration (Anzaldúa et al. ; Baranda‐Avila et al. ), a lack of affectation of them could be predicted.…”

Section: Discussionmentioning

confidence: 99%

Hypothyroidism Affects Differentially the Cell Size of Epithelial Cells Among Oviductal Regions of Rabbits

Anaya-Hernández

Rodríguez-Castelán

Nicolás

et al. 2014

Reprod Domestic Animals

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Oviductal regions show particular histological characteristics and functions. Tubal pathologies and hypothyroidism are related to primary and secondary infertility. The impact of hypothyroidism on the histological characteristics of oviductal regions has been scarcely studied. Our aim was to analyse the histological characteristics of oviductal regions in control and hypothyroid rabbits. Hypothyroidism was induced by oral administration of methimazole (MMI) for 30 days. For both groups, serum concentrations of thyroid and gonadal hormones were determined. Sections of oviductal regions were stained with the Masson's trichrome technique to analyse both epithelial and smooth muscle layers. The percentage of proliferative epithelial cells (anti-Ki67) in diverse oviductal regions was also quantified. Data were compared with Student t-test, Mann-Whitney U-test, or Fischer's test. In comparison with the control group, the hypothyroid group showed: (i) a low concentration of T3 and T4, but a high level of TSH; (ii) similar values of serum estradiol, progesterone and testosterone; (iii) a large size of ciliated cells in the ampulla (AMP), isthmus (IST) and utero-tubal junction (UTJ); (iv) a large size of secretory cells in the IST region; (v) a low percentage of proliferative secretory cells in the fimbria-infundibulum (FIM-INF) region; and (vi) a similar thickness of the smooth muscle layer and the cross-sectional area in the AMP and IST regions. Modifications in the size of the oviductal epithelium in hypothyroid rabbits could be related to changes in the cell metabolism that may impact on the reproductive functions achieved by oviduct.

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Section: Discussionmentioning

confidence: 99%

Hypothyroidism Affects Differentially the Cell Size of Epithelial Cells Among Oviductal Regions of Rabbits

Anaya-Hernández

Rodríguez-Castelán

Nicolás

et al. 2014

Reprod Domestic Animals

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“…In women, the contraceptive actions of progestins generally include suppression of the gonadotropin surges and luteal serum progesterone concentration, inhibition of progesterone receptor synthesis in the endometrium, and inhibition of final preovulatory oocyte maturation, leading to suppressed ovulation [14][15][16]. Isoforms of the progesterone receptor have been identified in the brain and reproductive tissues, including ovaries, testes, and oviducts, in amphibians as well as in humans and other mammals [17][18][19][20][21][22][23][24][25][26][27]. In light of the global decline of amphibians and their proven sensitivity to endocrine disruption [28][29][30][31] it is important to increase our understanding of amphibians' susceptibility to adverse effects of progestogenic environmental pollutants.…”

Section: Introductionmentioning

confidence: 99%

Disrupted Oogenesis in the Frog Xenopus tropicalis after Exposure to Environmental Progestin Concentrations1

Säfholm

Norder

Fick

et al. 2012

Biology of Reproduction

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Levonorgestrel is a synthetic progesterone commonly used in pharmaceuticals (e.g., in contraceptives). It is found in sewage treatment plant effluents at concentrations up to 30 ng/L and was recently shown to pose a threat to egg laying in fish. Information on the susceptibility of adult amphibians to progestin toxicity is lacking. The present study aimed to 1) characterize progestogenic effects on the full cycle of oogenesis (egg development) in frogs and 2) determine female amphibians' susceptibility to reproductive impacts from progestogenic compounds in the environment. Sexually mature female Xenopus tropicalis were exposed to levonorgestrel via the surrounding water for 7 days (0, 51, or 307 ng/L) or 28 days (0, 1.3, 18, 160, or 1240 ng/L). Their ovaries were analyzed histologically with respect to frequencies of immature (in early meiotic prophase I), previtellogenic, vitellogenic, mature, and atretic oocytes. The 28-day exposure caused reduced proportions of oocytes at immature, vitellogenic, and mature stages, and increased proportions of previtellogenic oocytes compared with the control. The lowest tested concentration, 1.3 ng/L, increased the proportions of previtellogenic oocytes and reduced the proportions of vitellogenic oocytes, indicating inhibited vitellogenesis. The present study shows that progestin concentrations found in the aquatic environment impaired oogenesis in adult frogs. Our results indicate that progestogenic effects on oocyte development include interrupted germ cell progression into meiosis and inhibited vitellogenesis. Considering the crucial role of oogenesis in female fertility, our results indicate that progestogenic pollutants may pose a threat to reproduction in wild amphibian populations.

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“…The oviduct in mammals expresses receptors for gonadal steroids like estrogen (Garcia-Palencia et al 2007;Shao et al 2007) and progesterone in several isoforms during cyclicity (Briton-Jones et al 2005;Peralta et al 2005) and early pregnancy (Anzaldua et al 2007). The presence of the receptor alone provides an indication towards functional involvement of their respective ligand (estrogen/progesterone) in processes Figure 3 Representative photomicrographs of oviductal sections (ampullary region) identifying cellular localization of GnRHR (visualized with DAB showing brown staining) in rats during different time points of pregnancy.…”

Section: The Journal Of Histochemistry and Cytochemistrymentioning

confidence: 99%

Expression and Localization of Gonadotropin-releasing Hormone Receptor in the Rat Oviduct During Pregnancy

Sengupta

Sridaran

2007

J Histochem Cytochem.

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S U M M A R Y A recent study from our laboratory has shown cellular and ultrastructural distribution of the gonadotropin-releasing hormone (GnRH) and the relative expression of its mRNA in the rat oviduct during the postimplantation period of pregnancy (days 7, 9, 16, and 20). To determine the possible autocrine/paracrine involvement of the oviductal GnRH during pregnancy in rats, the present investigation aims at the study of the relative expression of GnRH receptor (GnRHR) mRNA by real-time PCR followed by immunolocalization of the peptide in the oviduct during pregnancy. Semiquantitative analysis of the oviductal GnRHR expression by Western blot was done by densitometry of the signal intensity. Our results indicate the expression of GnRHR mRNA in the rat oviduct throughout the postimplantation period of pregnancy with no significant difference in expression between the selected time points. Immunoreactive GnRHR peptide was localized predominantly in the cytoplasm of the luminal epithelial cells, with less expression in the cytoplasm of the stromal cells and the smooth muscles throughout the oviduct. Signal intensity of GnRHR was significantly lower during day 16 when compared to days 7 and 20. These results, for the first time, support the transcription of GnRHR mRNA and its translation to protein in the rat oviduct throughout the postimplantation period of pregnancy. The lower protein content of GnRHR by day 16 may be indicative of ligand-induced downregulation of the receptor expression. The present investigation thus strengthens our previously postulated hypothesis regarding the receptormediated autocrine/paracrine role of oviductal GnRH during pregnancy in rats.

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Regional differences in expression of progesterone receptor in oviduct and uterus of rabbit during early pregnancy

Cited by 18 publications

References 27 publications

Hypothyroidism Affects Differentially the Cell Size of Epithelial Cells Among Oviductal Regions of Rabbits

Hypothyroidism Affects Differentially the Cell Size of Epithelial Cells Among Oviductal Regions of Rabbits

Disrupted Oogenesis in the Frog Xenopus tropicalis after Exposure to Environmental Progestin Concentrations1

Expression and Localization of Gonadotropin-releasing Hormone Receptor in the Rat Oviduct During Pregnancy

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