Regional Differences in Chronic Stress-induced Alterations in Mast Cell and Protease-activated Receptor-2-positive Cell Numbers in the Colon of Ws/Ws Rats

Kim, Yong Sung; Lee, Moon Young; Ryu, Han Seung; Choi, Eul Sig; Oh, Jung Taek; Yun, Ki Jung; Choi, Suck Chei

doi:10.5056/jnm.2014.20.1.54

Cited by 10 publications

(13 citation statements)

References 35 publications

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“…In the present study, WAS exposure induced a transient, yet significant, increase in micturition frequency and total urine output only in NMS mice. Strikingly, neither NMS nor WAS had effects on fecal output, increased rates of which have been reported in animal models of IBS in males, a phenotype sensitive to CRF antagonism (Buckley et al, 2014; Kim et al, 2014; Rho et al, 2014; Suda et al, 2013). …”

Section: Discussionmentioning

confidence: 75%

Urinary bladder hypersensitivity and dysfunction in female mice following early life and adult stress

et al. 2016

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Early adverse events have been shown to increase the incidence of interstitial cystitis/painful bladder syndrome in adulthood. Despite high clinical relevance and reports of stress-related symptom exacerbation, animal models investigating the contribution of early life stress to female urological pain are lacking. We examined the impact of neonatal maternal separation (NMS) on bladder sensitivity and visceral neuroimmune status both prior-to, and following, water avoidance stress (WAS) in adult female mice. The visceromotor response to urinary bladder distension was increased at baseline and 8d post-WAS in NMS mice, while colorectal sensitivity was transiently increased 1d post-WAS only in naïve mice. Bladder micturition rate and output, but not fecal output, were also significantly increased following WAS in NMS mice. Changes in gene expression involved in regulating the stress response system were observed at baseline and following WAS in NMS mice, and WAS reduced serum corticosterone levels. Cytokine and growth factor mRNA levels in the bladder, and to a lesser extent in the colon, were significantly impacted by NMS and WAS. Peripheral mRNA levels of stress-responsive receptors were differentially influenced by early life and adult stress in bladder, but not colon, of naïve and NMS mice. Histological evidence of mast cell degranulation was increased in NMS bladder, while protein levels of protease activated receptor 2 (PAR2) and transient receptor potential ankyrin 1 (TRPA1) were increased by WAS. Together, this study provides new insight into mechanisms contributing to stress associated symptom onset or exacerbation in patients exposed to early life stress.

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Section: Discussionmentioning

confidence: 75%

Urinary bladder hypersensitivity and dysfunction in female mice following early life and adult stress

et al. 2016

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“…Psychological stress is known to be closely associated with the onset and deterioration of IBS, and this association is greater in females. Exposure to acute or chronic stress affects bowel function and mast cell activation [ 25 ]. There have been many studies on contribution of mast cells to stress-induced gut dysfunction [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Probiotics reduce repeated water avoidance stress-induced colonic microinflammation in Wistar rats in a sex-specific manner

et al. 2017

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The colonic response to stress is greater in female rats than in male rats. The aim of this study was to evaluate the effect of probiotics in the repeated water avoidance stress (rWAS)-induced colonic microinflammation model of Wistar rats in a sex-specific manner. The three groups (no-stress, WAS, and WAS with probiotics) were exposed to r-WAS for 1 h daily for 10 days, and Lactobacillus farciminis was administered by oral gavage for 10 days to animals in the probiotics group. The visceromotor response (VMR) to colorectal distension (CRD) was assessed using a barostat and noninvasive manometry before and after WAS exposure. Immunohistochemistry for mast cells and real-time polymerase chain reaction (RT-PCR) for detection of mucosal cytokines were performed using distal colon tissue after the animals were sacrificed. Significant reduction of VMR to CRD (visceral analgesia) was observed at 60 mmHg in the female WAS group (P = 0.045), but not in males. In addition, the female WAS with probiotics group showed a significantly lower colonic mucosal mast cell count in comparison to the female WAS group (P = 0.013), but this phenomenon was not observed in the male group. The colonic mucosal mRNA levels of interferon-γ (IFNR), tumor necrosis factor-α (TNFA), interleukin (IL) 6, and IL17 were higher in the female WAS group than in the male WAS group. The mRNA levels of IFNR, TNFA, and IL6 were significantly decreased in WAS females who received probiotics (all P < 0.050). In conclusion, rWAS is induced in a sex-specific manner. A 10-day-long treatment with L. farciminis is an effective therapy for rWAS-induced colonic microinflammation in female rates, but not in male rats.

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“…The increase was mediated partly by mechanisms including, muscarinic and nicotinic pathways, activation and number of mast cells and the release of nerve growth factor. 79 Stress-induced enhancement of colonic permeability was mimicked by exogenous administration of CRF, 33 and abolished by pretreatment with the peripheral administration of the non-selective CRF antagonists astressin or -helical CRF 9-41 . 37,55,80 Likely, the selective CRF 1 receptor agonist, cortagine, 48 the selective CRF 1 receptor antagonist, SSR-125543, 80 and the selective CRF 2 receptor antagonist, antisauvagine-30, 78 reduced the response, supporting the participation of both CRF receptors in the modulation of colonic permeability.…”

Section: Permeabilitymentioning

confidence: 99%

“…23 This increase was glucocorticoid-mediated because adrenalectomy and pharmacologic blockade of glucocorticoid receptors inhibited the response, and dexamethasone increased gastrointestinal permeability in control rats. 23 Similarly, rats models of chronic stress (maternal deprivation, 55,[77][78][79] WAS, 45 and crowding stress 52 ) also show increased ionic permeability, and macromolecular permeability throughout the intestine. The increase was mediated partly by mechanisms including, muscarinic and nicotinic pathways, activation and number of mast cells and the release of nerve growth factor.…”

Section: Permeabilitymentioning

confidence: 99%

Role of Corticotropin-releasing Factor in Gastrointestinal Permeability

Rodiño-Janeiro¹,

Alonso-Cotoner²,

Pigrau³

et al. 2015

J Neurogastroenterol Motil

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The interface between the intestinal lumen and the mucosa is the location where the majority of ingested immunogenic particles face the scrutiny of the vast gastrointestinal immune system. Upon regular physiological conditions, the intestinal microflora and the epithelial barrier are well prepared to process daily a huge amount of food-derived antigens and non-immunogenic particles. Similarly, they are ready to prevent environmental toxins and microbial antigens to penetrate further and interact with the mucosal-associated immune system. These functions promote the development of proper immune responses and oral tolerance and prevent disease and inflammation. Brain-gut axis structures participate in the processing and execution of response signals to external and internal stimuli. The brain-gut axis integrates local and distant regulatory networks and supersystems that serve key housekeeping physiological functions including the balanced functioning of the intestinal barrier. Disturbance of the brain-gut axis may induce intestinal barrier dysfunction, increasing the risk of uncontrolled immunological reactions, which may indeed trigger transient mucosal inflammation and gut disease. There is a large body of evidence indicating that stress, through the brain-gut axis, may cause intestinal barrier dysfunction, mainly via the systemic and peripheral release of corticotropin-releasing factor. In this review, we describe the role of stress and corticotropin-releasing factor in the regulation of gastrointestinal permeability, and discuss the link to both health and pathological conditions. (J Neurogastroenterol Motil 2015;21:33-50)

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Regional Differences in Chronic Stress-induced Alterations in Mast Cell and Protease-activated Receptor-2-positive Cell Numbers in the Colon of Ws/Ws Rats

Cited by 10 publications

References 35 publications

Urinary bladder hypersensitivity and dysfunction in female mice following early life and adult stress

Urinary bladder hypersensitivity and dysfunction in female mice following early life and adult stress

Probiotics reduce repeated water avoidance stress-induced colonic microinflammation in Wistar rats in a sex-specific manner

Role of Corticotropin-releasing Factor in Gastrointestinal Permeability

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