Regional Deficits of Myocardial Blood Flow and Function in Left Ventricular Pacing–Induced Heart Failure

Helmer, Gregory; McKirnan, M. Dan; Shabetai, Ralph; Boss, Gerry R.; Ross, John; Hammond, H. Kirk

doi:10.1161/01.cir.94.9.2260

Cited by 49 publications

(30 citation statements)

References 27 publications

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“…Regionally, the lateral wall showed less systolic dysfunction (measured by percent systolic wall thickening) in animals that received Ad.AC VI (Pϭ0.048; Table 3). The IVS was not affected as much by pacing as reported previously 6 and was invariant between groups. Global LV function was also improved in animals receiving Ad.AC VI .…”

Section: Contractile Functionsupporting

confidence: 78%

“…Details of the surgical procedures have been reported previously. 6 Catheters were placed in the LV, aorta, pulmonary artery, and left atrium, and a high-fidelity pressure transducer was placed in the LV chamber (Konigsberg). LV epicardial pacing (Prevail 8086, Medtronic Inc) was used as reported previously.…”

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

“…LV epicardial pacing (Prevail 8086, Medtronic Inc) was used as reported previously. 6 Thirteen days later, measures of hemodynamics, LV global and regional function (echocardiography), and contractile responses to graded doses of isoproterenol and dobutamine (assessed by pressure transducers implanted in the LV chamber) were made. Pacemakers were then activated (220 bpm, 5 V, 0.5 ms), and LV pacing was continued for the 21-day duration of the study (Figure 1).…”

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

“…6,9 Images were recorded with animals in a basal state and during dobutamine infusion (20 g · kg Ϫ1 · min Ϫ1 ). These studies were performed 13 days after instrumentation, 1 or 2 days before gene transfer, and after 3 weeks of pacing.…”

Section: Echocardiographic Assessment Of Global and Regional Contractmentioning

confidence: 99%

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Intracoronary Adenovirus Encoding Adenylyl Cyclase VI Increases Left Ventricular Function in Heart Failure

et al. 2004

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Background-We tested the hypothesis that intracoronary delivery of an adenovirus encoding adenylyl cyclase type VI (Ad.AC VI ) would be associated with increased left ventricular (LV) function in pigs with congestive heart failure. Methods and Results-Pigs (52Ϯ6 kg; nϭ16) underwent placement of pacemakers, LV pressure transducers, and left atrial and aortic catheters. Physiological and echocardiographic studies were obtained from conscious animals 13 days later, and pacing was initiated (220 bpm). Seven days later, measures of LV function were reduced, documenting severe LV dysfunction and dilation. Pigs then received intracoronary Ad.AC VI (1.4ϫ10 12 vp; nϭ7) or saline (PBS; nϭ9) (randomized, blinded), with concomitant infusion of nitroprusside (50 g/min, 6.4 minutes) to increase gene transfer. Pacing was continued for 14 days, and final studies were obtained. The a priori key end point was change in LV dP/dt during isoproterenol infusion (pre-Ad.AC VI value minus value after 21 days of pacing). Pigs receiving Ad.AC VI showed a smaller decrease in both LV ϩdP/dt (Pϭ0.0014) and LV ϪdP/dt (Pϭ0.0008). Serial echocardiography showed that Ad.AC VI treatment was associated with increased LV function and reduced LV dilation and that end-systolic wall stress was reduced. AC-stimulated cAMP production was increased 1.7-fold in LV samples from Ad.AC VI -treated pigs (Pϭ0.006), and B-type natriuretic peptide was reduced (0.035). Gene transfer was confirmed by polymerase chain reaction. Conclusions-AC

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Section: Contractile Functionsupporting

confidence: 78%

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

Section: Echocardiographic Assessment Of Global and Regional Contractmentioning

confidence: 99%

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Intracoronary Adenovirus Encoding Adenylyl Cyclase VI Increases Left Ventricular Function in Heart Failure

et al. 2004

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“…There is no evidence of overt ischemia in the model, but because of the reduced subendocardial blood flow per beat, regional ischemia at the pacing site with persistent stunning after discontinuation of pacing has been proposed as a potential mechanism. 74 Increased oxidative stress is thought to causally contribute to activation of apoptosis 67 and to the oxidative modification of the contractile machinery. 24 …”

mentioning

confidence: 99%

Heart Rate and Heart Failure - Not a Simple Relationship -

Heusch

2011

Circ J

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Changes in myocardial protein expression in pacing-induced canine heart failure

Heinke

Wheeler²,

Yan³

et al. 1999

Electrophoresis

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Canine rapid ventricular pacing produces a low output cardiomyopathic state which is similar to dilated cardiomyopathy. In this study dogs were paced at 245 beats per minute (bpm) for 3—4 weeks until signs of heart failure were apparent. Unpaced dogs were used as controls. A previous study identified myocardial protein changes in the pH region 4—7 following ventricular pacing by using two‐dimensional electrophoresis (2‐DE) (Heinke et al., Electrophoresis 1998 19, 2021—2030). Many of these proteins were associated with mitochondria, energy metabolism within the cardiomyocyte, the cytoskeleton and calcium cycling. The present study aimed to examine the proteins migrating in the more basic region of the 2‐DE pattern using immobilised pH gradient 3—10 strips to separate myocardial proteins. The expression of 31 proteins was altered in the paced myocardium: 21 were decreased and 10 increased. Following the identification of 23 of these spots by either amino acid compositional analysis or peptide mass fingerprinting or a combination of both, we confirm that many of the proteins whose expression is altered following ventricular pacing are associated with the mitochondria and energy production within the cardiomyocyte, including creatine kinase M, triosephosphate isomerase, phosphoglycerate mutase, cytochrome c oxidase, cytochrome b5, hydroxymethyl glutaryl CoA synthase, myoglobin, and 3,2‐trans‐enoyl‐CoA transferase. Additionally, the cytoskeletal protein actin was increased in the paced hearts. These results strongly support the notion that energy production is impaired and mitochondrial dysfunction is involved in the development of heart failure in the paced dog.

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Regional Deficits of Myocardial Blood Flow and Function in Left Ventricular Pacing–Induced Heart Failure

Cited by 49 publications

References 27 publications

Intracoronary Adenovirus Encoding Adenylyl Cyclase VI Increases Left Ventricular Function in Heart Failure

Intracoronary Adenovirus Encoding Adenylyl Cyclase VI Increases Left Ventricular Function in Heart Failure

Heart Rate and Heart Failure - Not a Simple Relationship -

Changes in myocardial protein expression in pacing-induced canine heart failure

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