Regional blood flows measured in Mongolian gerbil by a modified microsphere method

Matsumoto, M; Kimura, Kaoru; Fujisawa, Atsushi; Matsuyama, Tomoo; Asai, Takeshi; Uyama, Osamu; Yoneda, S; Abe, Hideaki

doi:10.1152/ajpheart.1982.242.6.h990

Cited by 14 publications

(11 citation statements)

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“…Blood samples were obtained directly from the heart as described previously, 13 with some modifications. Briefly, mice were anesthetized with 0.5% halothane.…”

Section: Blood Gas Analysismentioning

confidence: 99%

The 150-Kilodalton Oxygen-Regulated Protein Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice

Nakagomi

Kitada

Kuribayashi

et al. 2004

The American Journal of Pathology

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The 150-kd oxygen-regulated protein is a novel stress protein that is located in the endoplasmic reticulum and contributes to cell survival when this organelle is under stress. Expression of this protein was strongly increased in alveolar macrophages and alveolar epithelial cells from mice with acute lung injury induced by lipopolysaccharide. Transgenic mice overexpressing the 150-kd protein showed decreased histological severity of this lung injury, accompanied by lower total protein concentrations, and lactate dehydrogenase activity in bronchoalveolar lavage fluid. As indicated by nick end-labeling, lipopolysaccharide induced apoptosis in fewer alveolar wall cells in transgenic than in wild-type mice. Transgenic mice also showed increased survival after lipopolysaccharide injection (a log-rank test). Thus, the 150-kd protein, an endoplasmic reticulum-related molecular chaperone, is pivotal in resisting acute lung injury from lipopolysaccharide. Lipopolysaccharide (LPS), a toxic biological substance that initiates acute inflammation, can produce experimental acute lung injury (ALI) 1-3 resulting in hypoxemia. Ongoing exposure to hypoxemia/hypoxia induces death of lung cells, which are vulnerable to hypoxic conditions; hypoxia further increases lung inflammation after LPS exposure.4,5 Thus, hypoxia may play a central role in LPS-induced ALI.Extreme hypoxia induces changes in cellular metabolic homeostasis. The 150-kd oxygen-regulated protein (ORP150/HSP12A), first cloned from cultured astrocytes under hypoxic conditions, 6 is a molecular chaperone belonging to the heat shock protein (HSP) family. Located in the endoplasmic reticulum (ER), ORP150 resembles glucose-regulated proteins (GRPs) such as GRP78 and GRP94. 7 ORP150 expression is required for cells to survive hypoxic conditions, indicating that ORP150 is important in protection of cells from hypoxia/ischemia. 8 In the central nervous system, ORP150 overexpression was found to protect neurons from ischemia by suppression of apoptosis 9 and/or activation of Ca 2ϩ -dependent proteinases. 10,11 These findings establish an important role for this ER-located molecular chaperone in neuronal defense mechanisms against environmental stress.Although ORP150 normally is expressed in most tissues, its role in the development of respiratory diseases has not been evaluated. Severity of ALI is associated with degree of alveolar epithelial cell injury.12 As alveolar epithelial cells participate in essential functions including gas exchange, they must be protected from environmental stresses including hypoxia. Thus, understanding of cellular defense mechanisms against such events could lead to therapeutic strategies that enhance protection of alveolar epithelial cells from pathogenetic insults. Induction of ER-associated chaperones such as ORP150 has not been studied in the respiratory system as a possible mechanism for cytoprotection.We used a mouse model of LPS-induced ALI to examine changes in expression of ORP150 by alveolar epithelial cells, and to determine whether OR...

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“…Blood samples were obtained directly from the heart as described previously, 13 with some modifications. Briefly, mice were anesthetized with 0.5% halothane.…”

Section: Blood Gas Analysismentioning

confidence: 99%

The 150-Kilodalton Oxygen-Regulated Protein Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice

Nakagomi

Kitada

Kuribayashi

et al. 2004

The American Journal of Pathology

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“…CBF has been measured after unilateral carotid occlusion in gerbils. 9 '' 4 -17 While focal CBF values after unilateral CCA occlusion were variable in symptomatic gerbils if unselected," they were not variable in our investigation once each gerbil was classified according to clinical status. This was particularly true in severely symptomatic gerbils.…”

Section: Discussionmentioning

confidence: 67%

“…ketamine hydrochloride, and the tail artery and the right saphenous vein were cannulated with PE-10 polyethylene catheters filled with 50 IU heparin/ml saline and sealed. 9 The right CCA was reexposed and looped with a 5-0 silk suture for future ligation, and the wound was closed. Several hours later, each gerbil was lightly anesthetized with ether inhalation and the right CCA was ligated as the gerbil emerged from brief anesthesia.…”

Section: Measurement Of Focal Cerebral Blood Flowmentioning

confidence: 99%

Prediction of stroke before and after unilateral occlusion of the common carotid artery in gerbils.

Matsumoto

Hatakeyama

Akai

et al. 1988

Stroke

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A method was developed to predict the severity of cerebral ischemia before permanent occlusion of a common carotid artery in gerbils by observing the diameter and appearance of the artery after temporary occlusion and observing clinical signs after permanent occlusion. The severity of cerebral ischemia was confirmed by a sensitive immunohistochemical method and measurement of focal cerebral blood flow after 30 minutes' ischemia. All gerbils with > 40% reduction of the diameter and a white arterial margin distal to temporary occlusion developed severe neurologic signs following permanent occlusion, but no gerbils with reduction of <30% and a red arterial margin developed neurologic signs. With the cumulative neurologic score, gerbils could be divided into classes with no, mild, moderate, and severe symptoms, mostly after 10 minutes. Severely symptomatic gerbils were identified in 3 minutes. Extensive ischemic damage was observed in severely symptomatic gerbils, but no immunohistochemical lesion was detected in mildly symptomatic gerbils. Cerebral blood flow was markedly reduced in severely symptomatic gerbils but more selectively reduced in the cortical structures of moderately symptomatic gerbils. This prediction method is useful for investigating early cerebral ischemia and for evaluating the effectiveness of pharmacologic agents.

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“…Reference measurements were obtained using a radiolabeled-microsphere technique [5][6]. Immediately following US evaluation, radiolabeled microspheres were injected into the proximal ascending aorta and a reference arterial blood sample was obtained for subsequent determination of true testicular perfusion.…”

Section: B Regional Perfusion Measurementsmentioning

confidence: 99%

Contrast-enhanced, real-time volumetric imaging of tissue perfusion

Clement

Paltiel

2010

2010 IEEE International Ultrasonics Symposium

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Although conventional contrast-enhanced harmonic US imaging is typically employed in subjective assessments, quantitative analysis is not widely performed. We have been developing a 3D mapping technique that may offer such assessment by recording transient changes in backscatter over a volume during controlled constant infusion of contrast. Unlike perfusion-refill techniques, the approach does not require pulseinversion imaging, nor does it perform bubble destruction using a high-intensity pulse. The goal of the present study is to correlate the method with a gold-standard in vivo. Experiments were performed in 20 rabbits during intravenous infusion of Definity® before and after unilateral testicular torsion and contralateral orchiopexy. The use of the testicular model allowed the unconstricted testicle to serve as a control. The degree of torsion was 0° in 4 (sham surgery), 180° in 4, 360° in 4, 540° in 4, and 720° in 4. Signals were recorded using a 3D imaging array and analyzed offline. For comparison radiolabeled microsphere measurements, an accepted reference standard, were also acquired. Correlation of all US testicular signal intensity ratios versus all radiolabeled microsphere perfusion ratios demonstrated a correlation coefficient of .79 (p < 0.0001), while postoperative values revealed a coefficient of .90 (p < 0.0001).

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Regional blood flows measured in Mongolian gerbil by a modified microsphere method

Cited by 14 publications

References 0 publications

The 150-Kilodalton Oxygen-Regulated Protein Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice

The 150-Kilodalton Oxygen-Regulated Protein Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice

Prediction of stroke before and after unilateral occlusion of the common carotid artery in gerbils.

Contrast-enhanced, real-time volumetric imaging of tissue perfusion

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