Regional blood flow and strontium‐85 incorporation rate in the rat hindlimb skeleton

Kirkeby, Ole J.; Berg‐Larsen, Trine

doi:10.1002/jor.1100090612

Cited by 23 publications

(10 citation statements)

References 26 publications

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“…A positive linear correlation between vascularisation and bone formation has been demonstrated in intact and fractured bone [37,44] and in fresh grafts [15,39]. In concordance with the results of these studies, we found a moderately high linear correlation between 85 Sr and 141 Ce radioactivity in intact host iliac bone (r = 0.75, p = 0.0001) and in recovered fresh syngeneic grafts (r = 0.50, p = 0.001).…”

Section: Discussionsupporting

confidence: 78%

The Relationship between Revascularisation and Osteogenesis in Fresh or Demineralised Bone Grafts

Solheim

Pinholt²,

Talsnes³

et al. 2001

Eur Surg Res

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Bone formation generally depends on adequate blood flow. Failure of bone grafts has been attributed to delayed revascularisation of the graft. We compared the relationship between revascularisation and osteogenesis, evaluated as entrapment of ¹⁴¹Ce-labelled microspheres and uptake of ⁸⁵Sr, respectively, in fresh or demineralised syngeneic bone grafts 3 weeks after heterotopic implantation in rats. Whereas a moderately high linear correlation between ⁸⁵Sr and ¹⁴¹Ce radioactivity was found both in the (intact) host iliac bone (r = 0.75, p = 0.0001) and implanted fresh syngeneic grafts (r = 0.50, p = 0.001), no correlation could be demonstrated in demineralised grafts (r = 0.09, p = 0.6). The results may indicate differences in the mechanisms of vascularisation and osteogenesis in the grafts used fresh or after demineralization but are, at present, difficult to fully explain.

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Section: Discussionsupporting

confidence: 78%

The Relationship between Revascularisation and Osteogenesis in Fresh or Demineralised Bone Grafts

Solheim

Pinholt²,

Talsnes³

et al. 2001

Eur Surg Res

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“…Upon injection into the animal, microspheres lodge in tissue capillaries in direct proportion to the fraction of cardiac output perfusing the tissue (McCarthy, 2006). Tissue analysis is relatively straightforward, even for mineralized tissue, in that once tissues of interest are harvested, blood flow is estimated via a gamma spectrometer with minimal tissue processing (Colleran et al, 2000, Kirkeby and Berg-Larsen, 1991, Schimmel et al, 2000, Stabley et al, 2013). Various limitations of radioactive microspheres, including cost, health risks, and the necessity for precautions with use and disposal (Glenny et al, 1993), have resulted in exploration of alternative methods.…”

Section: Introductionmentioning

confidence: 99%

Assessment of regional bone tissue perfusion in rats using fluorescent microspheres

2017

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Disturbances in bone blood flow have been shown to have deleterious effects on bone properties yet there remain many unanswered questions about skeletal perfusion in health and disease, partially due to the complexity of measurement methodologies. The goal of this study was use fluorescent microspheres in rats to assess regional bone perfusion by adapting mouse-specific fluorescent microsphere protocol. Ten fifteen-week old Sprague Dawley rats were injected with fluorescent microspheres either via cardiac injection (n = 5) or via tail vein injection (n = 5). Femora and tibiae were harvested and processed to determine tissue fluorescence density (TFD) which is proportional to the number of spheres trapped in the tissue capillaries. Right and left total femoral TFD (2.77 ± 0.38 and 2.70 ± 0.24, respectively) and right and left tibial TFD (1.11 ± 0.26 and 1.08 ± 0.34, respectively) displayed bilateral symmetry in flow when assessed in cardiac injected animals. Partitioning of the bone perfusion into three segments along the length of the bone showed the distal femur and proximal tibia received the greatest amount of perfusion within their respective bones. Tail vein injection resulted in unacceptably low TFD levels in the tibia from 4 of the 5 animals. In conclusion this report demonstrates the viability of cardiac injection of fluorescent microspheres to assess bone tissue perfusion in rats.

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“…Cortical bone blood flow has been shown to vary directly with the number of Haversian remodeling foci [26]. Moreover, the regional distribution of blood flow correlates with the incorporation rate of strontium (calcium analog) in rat bone [22]. A similar close relationship between blood vessels, blood flow and trabecular bone turnover is very likely, but has been less well investigated.…”

Section: Introductionmentioning

confidence: 99%

Effects of oophorectomy on functional properties of resistance arteries isolated from the cancellous bone of the rabbit femur

Hansen

Forman

Lundgaard

et al. 2001

Journal Orthopaedic Research

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Information is sparse concerning the effect of oophorectomy (OOX) on bone vascularization and blood flow of possible significance for altered remodeling. Whether OOX affects functional characteristics of isolated bone resistance arteries was investigated. Ring preparations (diameter -250 pm) of small femoral bone arteries from oophorectomized and sham-operated rabbits were mounted on a myograph six weeks postoperatively. Cumulative concentration-response curves were obtained for various agonists at a normalized lumen diameter. Oophorectomy did not significantly influence lumen diameter, maximal response to high potassium, or maximal response to high potassium and M noradrenaline. However, OOX significantly increased the maximal response to noradrenaline (OOX 2.14 * 0.36 N/m, Sham 1.25 * 0.14 Nlm) and endothelin-1 (OOX 1.76 0.32, Sham 0.95 3~ 0.15) in metaphyseal arteries. Moreover, the corresponding maximal active pressure for the agonists was significantly increased. OOX did not influence endothelial function assessed by the effects of acetylcholine or substance P. The functional responses of diaphyseal arteries were unaffected by OOX. The study demonstrates regional differences in the effects of OOX on small arteries of importance for control of vascular resistance in bone which suggests a relation between altered vascular function after ovarian hormonal withdrawal and the changes in bone turnover associated with osteoporosis.

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Regional blood flow and strontium‐85 incorporation rate in the rat hindlimb skeleton

Cited by 23 publications

References 26 publications

The Relationship between Revascularisation and Osteogenesis in Fresh or Demineralised Bone Grafts

The Relationship between Revascularisation and Osteogenesis in Fresh or Demineralised Bone Grafts

Assessment of regional bone tissue perfusion in rats using fluorescent microspheres

Effects of oophorectomy on functional properties of resistance arteries isolated from the cancellous bone of the rabbit femur

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