Regional assignment of genes on human chromosomes 11 and 12

Kao, Fa Ten; Jones, Cynthia; Law, Lloyd W.; Puck, Theodore T.

doi:10.1159/000131001

Cited by 8 publications

(1 citation statement)

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“…In a panel of mouse-human somatic cell hybrids the homologous sequences in human genomic DNA segregated with human chromosome 11. This suggests that the phosphatase encoded by CT29 is identical with the human acid phosphatase ACP2, which has been localized to chromosome 11 using electrophoretic characterization of acid phosphatase activity in somatic cell hybrids (Bruns and Gerald, 1974;Busby et al, 1976;Shows et al, 1976;Jones and Kao, 1978;Kao et al, 1978). A homology of 60% was observed for the N-termini of LAP and human prostatic acid phosphatase.…”

Section: Discussionmentioning

confidence: 97%

Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment.

et al. 1988

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A 2112‐bp cDNA clone (lambda CT29) encoding the entire sequence of the human lysosomal acid phosphatase (EC 3.1.3.2) was isolated from a lambda gt11 human placenta cDNA library. The cDNA hybridized with a 2.3‐kb mRNA from human liver and HL‐60 promyelocytes. The gene for lysosomal acid phosphatase was localized to human chromosome 11. The cDNA includes a 12‐bp 5′ non‐coding region, an open reading frame of 1269 bp and an 831‐bp 3′ non‐coding region with a putative polyadenylation signal 25 bp upstream of a 3′ poly(A) tract. The deduced amino acid sequence reveals a putative signal sequence of 30 amino acids followed by a sequence of 393 amino acids that contains eight potential glycosylation sites and a hydrophobic region, which could function as a transmembrane domain. A 60% homology between the known 23 N‐terminal amino acid residues of human prostatic acid phosphatase and the N‐terminal sequence of lysosomal acid phosphatase suggests an evolutionary link between these two phosphatases. Insertion of the cDNA into the expression vector pSVL yielded a construct that encoded enzymatically active acid phosphatase in transfected monkey COS cells.

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Section: Discussionmentioning

confidence: 97%

Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment.

et al. 1988

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Encomium: Theodore puck, a life in biophysics applied to medicine

Robinson

1994

Am. J. Med. Genet.

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Ted Puck is a unique and outstanding individual. The accompanying autobiography is testament to the amount and variety of significant scientific work he has produced. There is no question that he has made seminal contributions to the understanding of human genetics, molecular biology, and health. As described in his autobiography, from the time he received his Ph.D. in 1940 he has been dedicated to the application of the quantitative principles of the physical sciences to problems of human biology, particularly those involving medicine. As an example of this desire to have an impact on medicine, in 1959 he offered me, a practicing pediatrician, a faculty position in his department. I was the only M.D. in the department, and we have collaborated closely and fruitfully ever since.An unusual thing about Ted is that at his current age of 78 he still continues to work excitedly on major problems of biology which affect medicine. At a time when most molecular biologists are isolating new genes and relating them to specific diseases, important as that is, Ted is trying t o quantitate and define the major problems of mutagenesis, differentiation, and carcinogenesis, all of which have a tremendous influence on human health.One of his most creative insights, which is the basis of what we today call somatic cell genetics, is his development of the "mammalian cell as a micro-organism.'' He has been aptly described as the "inventor of somatic cell genetics" [Editorial, Nature New Biology, 243:65, May 16, 19731. He has been constantly devoted to improving the quality of medical and graduate education. For years he ran a course for medical students in biophysics which conveyed to them the excitement and the rigors of the "new biology" of the 1960s. Both locally and nationally he has constantly pressed for modernizing medical education with greater emphasis on preventive medicine and the use of quantitative understanding rather than empiricism in diagnosis and treatment of disease.

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Irradiation microcell‐mediated chromosome transfer (XMMCT): The generation of specific chromosomal arm deletions

Dowdy

Scanlon

Fasching

et al. 1990

Genes Chromosomes & Cancer

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The microcell-mediated chromosome transfer technique has been used to introduce whole chromosomes into malignant cells and revert the tumorigenic phenotype. However, in most instances the limited availability of selectable chromosomes has hindered the ability to reduce the region containing the tumor suppressive information. The work presented here describes a new method to enrich for specific chromosomal arm deletions of selectable chromosomes and thereby more finely focus upon the genetic region of interest. The irradiation-microcell mediated chromosome transfer (XMMCT) technique involves the irradiation of microcells containing single human chromosomes followed by fusion to a nonirradiated host and cytogenetic characterization. The XMMCT procedure was performed on a microcell hybrid containing a der(11) as the only human chromosome. The resultant irradiated microcell hybrids were found to have deletions that ranged from simple interstitial deletions to complex deletions/rearrangements involving only the human der(11) chromosome. The XMMCT procedure has broad applications in generating chromosomal reagents for mapping genetic loci and for use in functional analyses such as tumor suppression studies.

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Regional assignment of genes on human chromosomes 11 and 12

Cited by 8 publications

References 0 publications

Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment.

Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment.

Encomium: Theodore puck, a life in biophysics applied to medicine

Irradiation microcell‐mediated chromosome transfer (XMMCT): The generation of specific chromosomal arm deletions

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