Regional and disease specific human lung extracellular matrix composition

Hoffman, Eileen; Uhl, Franziska E.; Asarian, Loredana; Deng, Bin; Becker, Chloe; Uriarte, Juan J.; Downs, Isaac; Young, Brad de; Weiss, Daniel J.

doi:10.1016/j.biomaterials.2022.121960

Cited by 20 publications

(33 citation statements)

References 73 publications

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“…52 Thirdly, proteomic analysis of ECM hydrogels can be carried out, for example, a potent technique to screen the complex milieu of proteins and peptides in a tissue or organ is the combination of 2D gel electrophoresis with mass spectrometry (MS). 53 Finally, some safety tests, such as histocompatibility and cytocompatibility tests, are also necessary. 54 3 Engineering of ECM hydrogels…”

Section: Reviewmentioning

confidence: 99%

The engineering and application of extracellular matrix hydrogels: a review

Zhang

2023

Biomater. Sci.

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Section: Reviewmentioning

confidence: 99%

The engineering and application of extracellular matrix hydrogels: a review

Zhang

2023

Biomater. Sci.

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“…Further, even though there remains significant room for innovation and improvement in lung de- and recellularization approaches, decellularized organs have proven to be valuable experimental tools to study diseased ECM and cell-ECM interactions. For example, decellularized lungs from patients with COPD or IPF have provided significant insight into disease pathogenesis and the role of the ECM and disordered cell-matrix interactions in disease processes ( Booth et al, 2012 ; Wagner et al, 2014b ; Ahrman et al, 2018 ; Hoffman et al, 2023 ).…”

Section: Overview Of Lung Regenerative Medicinementioning

confidence: 99%

“…Used in place of Matrigel™, the hydrogels are a unique culture model that provide a native and thus more relevant matrix. The lung-derived hydrogels can be produced not just from the entire lung but from airway, vascular, and alveolar-enriched regions obtained by dissection of decellularized lungs ( Hoffman et al, 2023 ), this providing an opportunity to investigate regional differences in cell-matrix interactions. Further, hydrogels can be produced from decellularized diseased lungs providing additional novel model investigative systems ( Petrou et al, 2020 ; Uhl et al, 2020 ; Saleh et al, 2022 ; Hoffman et al, 2023 ).…”

Section: Overview Of Lung Regenerative Medicinementioning

confidence: 99%

“…The lung-derived hydrogels can be produced not just from the entire lung but from airway, vascular, and alveolar-enriched regions obtained by dissection of decellularized lungs ( Hoffman et al, 2023 ), this providing an opportunity to investigate regional differences in cell-matrix interactions. Further, hydrogels can be produced from decellularized diseased lungs providing additional novel model investigative systems ( Petrou et al, 2020 ; Uhl et al, 2020 ; Saleh et al, 2022 ; Hoffman et al, 2023 ). These are powerful investigative tools that will provide novel information that in part can be utilized to improve recellularization schemes for decellularized lungs.…”

Section: Overview Of Lung Regenerative Medicinementioning

confidence: 99%

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What is the need and why is it time for innovative models for understanding lung repair and regeneration?

Weiss

2023

Front. Pharmacol.

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Advances in tissue engineering continue at a rapid pace and have provided novel methodologies and insights into normal cell and tissue homeostasis, disease pathogenesis, and new potential therapeutic strategies. The evolution of new techniques has particularly invigorated the field and span a range from novel organ and organoid technologies to increasingly sophisticated imaging modalities. This is particularly relevant for the field of lung biology and diseases as many lung diseases, including chronic obstructive pulmonary disease (COPD) and idiopathic fibrosis (IPF), among others, remain incurable with significant morbidity and mortality. Advances in lung regenerative medicine and engineering also offer new potential avenues for critical illnesses such as the acute respiratory distress syndrome (ARDS) which also continue to have significant morbidity and mortality. In this review, an overview of lung regenerative medicine with focus on current status of both structural and functional repair will be presented. This will serve as a platform for surveying innovative models and techniques for study, highlighting the need and timeliness for these approaches.

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“…Early proteomic studies ( Booth et al, 2012 ; Uhl et al, 2018 ) illustrated the retention of many components of the lung matrisome in decellularised scaffolds from control and diseased lung samples. A recent study from the team in the Weiss lab ( Hoffman et al, 2023 ), has elegantly shown that the components of the ECM are specific for different compartments within the lung (airways, alveolus, blood vessels), and that these change during chronic lung disease. These decellularized scaffolds from lung tissues have now been used as a source of ECM for the generation of hydrogels.…”

Section: The Possibilities With Lung Ecm-derived Hydrogelsmentioning

confidence: 99%

Current possibilities and future opportunities provided by three-dimensional lung ECM-derived hydrogels

Nizamoglu

Burgess

2023

Front. Pharmacol.

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Disruption of the complex interplay between cells and extracellular matrix (ECM), the scaffold that provides support, biochemical and biomechanical cues, is emerging as a key element underlying lung diseases. We readily acknowledge that the lung is a flexible, relatively soft tissue that is three dimensional (3D) in structure, hence a need exists to develop in vitro model systems that reflect these properties. Lung ECM-derived hydrogels have recently emerged as a model system that mimics native lung physiology; they contain most of the plethora of biochemical components in native lung, as well as reflecting the biomechanics of native tissue. Research investigating the contribution of cell:matrix interactions to acute and chronic lung diseases has begun adopting these models but has yet to harness their full potential. This perspective article provides insight about the latest advances in the development, modification, characterization and utilization of lung ECM-derived hydrogels. We highlight some opportunities for expanding research incorporating lung ECM-derived hydrogels and potential improvements for the current approaches. Expanding the capabilities of investigations using lung ECM-derived hydrogels is positioned at a cross roads of disciplines, the path to new and innovative strategies for unravelling disease underlying mechanisms will benefit greatly from interdisciplinary approaches. While challenges need to be addressed before the maximum potential can be unlocked, with the rapid pace at which this field is evolving, we are close to a future where faster, more efficient and safer drug development targeting the disrupted 3D microenvironment is possible using lung ECM-derived hydrogels.

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Regional and disease specific human lung extracellular matrix composition

Cited by 20 publications

References 73 publications

The engineering and application of extracellular matrix hydrogels: a review

The engineering and application of extracellular matrix hydrogels: a review

What is the need and why is it time for innovative models for understanding lung repair and regeneration?

Current possibilities and future opportunities provided by three-dimensional lung ECM-derived hydrogels

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