Regional and cellular expression of the mannose receptor in the post-natal developing mouse brain

Abstract

The mannose receptor, a glycoprotein expressed in a soluble and membrane form by macrophages, plays an important role in homeostasis and immunity. Using biochemical and immunohistochemical analyses, we demonstrate that this receptor, both in its soluble and membrane forms, is expressed in vivo in the post-natal murine brain and that its expression is developmentally regulated. Its expression is at its highest in the first week of life and dramatically decreases thereafter, being maintained at a low level throughout adulthood. The receptor is present in most brain regions at an early post-natal age, the site of the most intense expression being the meninges followed by the cerebral cortex, brain stem and the cerebellum. With age, expression of the mannose receptor is maintained in regions such as the cerebral cortex and the brain stem, whereas it disappears from others such as the hippocampus or the striatum. In healthy brain, no expression can be detected in oligodendrocytes, ependymal cells, endothelial cells or parenchymal microglia. The mannose receptor is expressed by perivascular macrophages/microglia and meningeal macrophages, where it might be important for the brain immune defence, and by two populations of endogenous brain cells, astrocytes and neurons. The developmentally dependent, regionally regulated expression of the mannose receptor in glial and neuronal cells strongly suggests that this receptor plays an important role in homeostasis during brain development and/or neuronal function.

Morphological Changes in the Brain of Mice with Systemic Candidiasis Treated with Composition of Amphotericin B and Oxidized Dextran

“…They participate in C. albicans adhesion to endothelial surface, probably BBB, along with other cell wall components. Thus, according to previous studies [5,8], mannose receptors were found on cerebral blood vessel endothelium. In addition, they are expressed on macrophages [8] actively phagocytizing fungi.…”

Ethanol impairs monosaccharide uptake and glycosylation in cultured rat astrocytes

“…Proteins were separated in SDS-polyacrylamide slabs gels using the discontinuous gel and buffer system of Laemmli (1970) with 8% polyacrylamide in the separate gel. After electrophoresis, the proteins were transferred to nitrocellulose paper, which was incubated for 60 min with a rabbit anti-GLUT1 polyclonal antibody (1 : 200), a rabbit anti-MR polyclonal antibody (1 : 5000) (Burudi et al 1999;Burudi and Regnier-Vigouroux 2001) or a rabbit anti-Man II polyclonal antibody (1: 2000) (Moremen and Robbins 1991) in TBS containing 0.05% Tween-20. Thereafter, the paper was incubated for 60 min with an anti-rabbit IgG-AP conjugate.…”

Selective targeting of perivascular macrophages for clearance of β-amyloid in cerebral amyloid angiopathy

“…Although macrophages are known to express the CD206 receptor, there are conflicting reports on cell-specific expression within the adult CNS (11,20). To confirm the localization of CD206 expression to perivascular macrophages in our mouse model, we performed double labeling immunocytochemistry with anti-CD206 receptor and anti-glial fibrillary acidic protein (GFAP) or antiionized calcium-binding adaptor molecule-1 (Iba1) antisera to rule out astrocyte and microglia expression, respectively.…”