“…Little would be gained with respect to such parameters by sampling more than a single point in a given preparation. Much of the interindividual variance in other variables measured, however, is an expression of regional differences within each explant, as is readily ascertainable by recording either sequentially (see Baker et al, 2006;Crain, 1976) or from several electrodes simultaneously (e.g., Plenz, 2003, 2004;Bettencourt et al, 2007;Chao et al, 2007;Droge et al, 1986;Giugliano et al, 2004;Jimbo and Robinson, 2000;Krause et al, 2006;Madhavan et al, 2007;Potter and DeMarse, 2001;Rolston et al, 2007;Wagenaar et al, 2006a,b). Thus, the mean duration, intensity and oscillatory character of a midi-or maxi-burst tends to be unpredictably site-specific, even in mega-co-cultures , thus giving each age and treatment group an irreducibly large variance with regard to those parameters (Echevarria and Albus, 2000;Pinato et al, 1999;Wagenaar et al, 2006a).…”