Regenerative Responses of Cyphomandra Betacea (Cav.) Sendt. (Tamarillo) Cultivated in Vitro

Obando, Melissa; Jordán, Miguel

doi:10.17660/actahortic.2001.560.83

Cited by 10 publications

(10 citation statements)

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“…betacea can be propagated by seeds or through conventional macropropagation techniques such as grafting or cuttings (Slack 1976;. In vitro protocols have also been applied, including axillary shoot proliferation (Obando and Jordan 2001), organogenesis (Atkinson and Gardner 1993) and somatic embryogenesis (Guimarães et al 1988;Lopes et al 2000;Canhoto et al 2005;Correia et al 2011. Somatic embryogenesis has proved to be particularly interesting since high rates of somatic embryo induction and plant conversion can be achieved both from juvenile and adult selected trees .…”

Section: Introductionmentioning

confidence: 99%

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Currais

Loureiro

Santos

et al. 2013

Plant Cell Tiss Organ Cult

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Ploidy levels of short-term (1 and 2 years) and long-term (7 and 10 years) embryogenic cultures as well as of regenerated plantlets of tamarillo were analyzed by flow cytometry and chromosome counts. Embryogenic cultures were induced from expanding leaves cultured in the presence of Picloram or 2,4-dichlorophenoxyacetic acid (2,4-D) and monthly subcultured on the same media. Embryo development and plantlets were obtained following subculture of the embryogenic tissue in auxin free medium containing gibberellic acid (GA 3). Seedlings and rooted shoots from axillary shoot proliferation were used as controls. The results showed that in long-term embryogenic cultures the ability to develop somatic embryos and plantlets was reduced. Embryogenic tissues maintained for 10 years were mostly aneuploids of the tetraploid (2n = 4x = 48) level whereas those kept in culture for 7 years or less were also mostly aneuploids but of the diploid (2n = 2x = 24) level. The results obtained by flow cytometry were, in general, consistent with those obtained by chromosome counts. The chromosome alteration observed in the embryogenic tissues was already present after 1 year of culture and increased with culture age, hence impairing the maintenance of these tissues for long periods without affecting chromosome stability of the regenerated plantlets. However, the occurrence of triploids and tetraploids as well as aneuploids can be useful for breeding purposes. A value around 23 pg/2C was found for the genome size of tamarillo largely exceeding the value previously published (15.50 pg/2C).

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Section: Introductionmentioning

confidence: 99%

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Currais

Loureiro

Santos

et al. 2013

Plant Cell Tiss Organ Cult

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“…In vitro propagation by axillary shoot proliferation (Barghchi 1986;Cohen and Elliot 1979), induction of organogenesis (Guimarães et al 1996;Obando and Jordan 2001) or somatic embryogenesis (Correia et al 2009) and genetic transformation techniques (Atkinson and Gardner 1993;Cohen et al 2000) are valuable biotechnological tools that have been applied to tamarillo breeding. SE is of particular interest because it has a great potential for the improvement of tree species and for rapid large-scale clonal propagation, genetic transformation and cryopreservation of desirable selected lines (Park 2002;von Arnold 2008).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis induction system for cloning an adult Cyphomandra betacea (Cav.) Sendt. (tamarillo)

Correia

Lopes

Canhoto

2011

Trees

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Somatic embryogenesis is a valuable tool for plant breeding. In recent years, different aspects related to somatic embryogenesis (SE) induction in tamarillo have been studied at our laboratory. In this work, results concerning the establishment of a protocol for cloning an adult tamarillo tree through SE are presented. Attempts to induce SE in tamarillo from various explants directly taken from an adult tree were unsuccessful and only calli with no embryogenic potential were initiated. To overcome the lack of potential of adult tissues for SE, an indirect approach was attempted in which shoots from an adult tree were first established in vitro and then wounded leaves were used for SE induction. A low rate of embryogenic tissue formation was obtained (19.4%), but it was in the range of initiation rates from leaf explants of in vitro cloned plantlets of different tamarillo cultivars (red, orange and yellow) that originated from a single seedling (13.3-54.4%). High variation in SE initiation among juvenile controls could not be explained by different organogenetic potential, as no significant differences in shoot proliferation or rooting ability during micropropagation could be detected. Subcultures of embryogenic lines from the adult tree allowed us to obtain a large amount of embryogenic tissue that, after 8 weeks on a PGR-free medium, gave an average of 111 plants per gram of fresh mass of embryogenic tissue. A RAPD comparative analysis of somatic embryo-derived plantlets and the donor tree confirmed that the plantlets had no variation in the DNA regions amplified by 12 primers. These results open the way for large-scale cloning of elite tamarillo trees through SE.

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“…An increasing number of studies describing in vitro propagation of Tamarillo using axillary buds have been reported (Barghchi, 1998;Obando et al, 1992;Waweru et al, 2011). Somatic embryogenesis has also been reported by Espinosa et al (2005), Obando and Jordan (2001), Correia et al (2009), Guimarães et al (1996), andCorreia et al (2011). However, there are no documented studies on the direct organogenesis in Tamarillo.…”

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confidence: 58%

A Novel Regeneration System for Tamarillo (Cyphomandra betacea) via Organogenesis from Hypocotyl, Leaf, and Root Explants

Kahia¹,

Sallah

Diby³

et al. 2015

horts

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Cyphomandra betacea (Cav.) is commonly known as Tamarillo or tree tomato. This species is mainly used for its edible fruits which have a high nutritional value and contain relatively high amounts of proteins, vitamins B6, C, E, and provitamin A. The cultivation of Tamarillo in Rwanda is facing major challenges caused mainly by viral diseases such as Tamarillo mosaic virus (TaMV). These diseases are difficult to control and are transferred through vegetative propagation, often resulting in heavy productivity losses and poor-quality fruits. Thus, this study was conducted to evaluate the possibility of using tissue culture as an alternative propagation method. Tamarillo seeds were sterilized using a commercial bleach and germinated in vitro to get clean starting explants. Explants (hypocotyls, leaves, and roots) were cultured on semisolid Murashige and Skoog (MS) media supplemented with 6-benzylaminopurine (BA), N6-2-isopentyl adenine (2iP), 6-furfurylaminopurine (kinetin) evaluated at 5, 10, 20, 40 µM, and thidiazuron (TDZ), evaluated at 0.1, 0.5. 1.0 1.5 µM in separate experiments. Data were collected on the number of microshoots and roots 2 months after culture and analyzed using the Statistical Software for Social Sciences (SPSS) Software version 8. The results showed that the growth regulators evaluated had a significant (P ≤ 0.05) effect on plantlet regeneration from leaf and hypocotyl explants. The media supplemented with BA 40 μM was the most effective in inducing multiple shoots from leaf explants producing 4.67 ± 0.15 shoots per explant. Root explants showed the least morphogenic responses for all the parameters evaluated. The regenerated plantlets were transplanted to the greenhouse and a survival rate of 90% was recorded. During this study, a simple, reproducible, single-step protocol was developed. These results would be useful for mass propagation of Tamarillo.

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Regenerative Responses of Cyphomandra Betacea (Cav.) Sendt. (Tamarillo) Cultivated in Vitro

Cited by 10 publications

References 0 publications

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Somatic embryogenesis induction system for cloning an adult Cyphomandra betacea (Cav.) Sendt. (tamarillo)

A Novel Regeneration System for Tamarillo (Cyphomandra betacea) via Organogenesis from Hypocotyl, Leaf, and Root Explants

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